Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E5A72_RS05130 Genome accession   NZ_CP039025
Coordinates   1048041..1048505 (-) Length   154 a.a.
NCBI ID   WP_000993723.1    Uniprot ID   -
Organism   Acinetobacter baumannii ATCC 17978 substr. PMR-High     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1043041..1053505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5A72_RS05105 (E5A72_05105) - 1043486..1044418 (-) 933 WP_000743271.1 IS5 family transposase -
  E5A72_RS20000 - 1044474..1044602 (+) 129 Protein_944 hypothetical protein -
  E5A72_RS05110 (E5A72_05110) - 1044723..1045106 (+) 384 WP_000090000.1 RidA family protein -
  E5A72_RS05115 (E5A72_05115) - 1045316..1045510 (+) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  E5A72_RS05120 (E5A72_05120) bfr 1045755..1046219 (+) 465 WP_000678124.1 bacterioferritin -
  E5A72_RS05125 (E5A72_05125) - 1046263..1047891 (-) 1629 WP_025467430.1 PglL family O-oligosaccharyltransferase -
  E5A72_RS05130 (E5A72_05130) pilA 1048041..1048505 (-) 465 WP_000993723.1 pilin Machinery gene
  E5A72_RS05135 (E5A72_05135) - 1048847..1049407 (-) 561 WP_000258957.1 TPM domain-containing protein -
  E5A72_RS05140 (E5A72_05140) - 1049401..1050483 (-) 1083 WP_001246375.1 TPM domain-containing protein -
  E5A72_RS05145 (E5A72_05145) - 1050505..1051095 (-) 591 WP_000846931.1 LemA family protein -
  E5A72_RS05150 (E5A72_05150) - 1051226..1052113 (-) 888 WP_001017325.1 metal-dependent hydrolase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15445.69 Da        Isoelectric Point: 8.1130

>NTDB_id=316714 E5A72_RS05130 WP_000993723.1 1048041..1048505(-) (pilA) [Acinetobacter baumannii ATCC 17978 substr. PMR-High]
MNAQKGFTLIELMIVVAIIGILAAIAIPQYQKYTARSQITAALAEISPGKTQFELALSEGTANSVNNNPAAIGLKSTTNN
CSAVNVTANGTTGTIACTLQGSATITGGVLTLTRSADVAAASGVNANVGGWTCSITKGTTDISSVIAPKGCTII

Nucleotide


Download         Length: 465 bp        

>NTDB_id=316714 E5A72_RS05130 WP_000993723.1 1048041..1048505(-) (pilA) [Acinetobacter baumannii ATCC 17978 substr. PMR-High]
ATGAATGCACAAAAAGGTTTTACATTAATTGAACTCATGATCGTGGTTGCCATTATCGGTATTTTGGCAGCAATTGCGAT
TCCGCAATATCAGAAATACACTGCTCGTTCTCAAATTACAGCAGCATTGGCGGAAATCAGCCCAGGGAAAACTCAATTTG
AACTAGCTCTATCCGAAGGTACAGCTAACAGTGTGAATAATAACCCCGCAGCAATTGGTTTAAAAAGTACTACTAATAAT
TGTTCTGCTGTTAATGTAACTGCAAATGGAACTACTGGTACTATTGCATGTACGTTACAAGGCTCTGCTACAATTACTGG
TGGTGTTCTAACTTTAACACGTAGTGCAGATGTAGCAGCTGCTTCTGGAGTAAATGCAAATGTGGGTGGCTGGACATGTT
CTATTACTAAAGGAACAACAGATATTTCTTCAGTAATTGCTCCAAAAGGCTGTACAATAATTTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-152)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

46.154

100

0.468
  pilA Pseudomonas aeruginosa PAK

42.073

100

0.448
  pilA Vibrio cholerae strain A1552

44.156

100

0.442
  pilA Vibrio cholerae C6706

44.156

100

0.442
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.156

100

0.442
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.333

97.403

0.442
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.529

88.312

0.429
  pilA Vibrio parahaemolyticus RIMD 2210633

39.333

97.403

0.383
  pilA Ralstonia pseudosolanacearum GMI1000

39.333

97.403

0.383
  pilA2 Legionella pneumophila str. Paris

42.963

87.662

0.377
  pilA2 Legionella pneumophila strain ERS1305867

42.963

87.662

0.377