Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LLW34_RS03380 Genome accession   NZ_CP032430
Coordinates   651959..652405 (+) Length   148 a.a.
NCBI ID   WP_228763864.1    Uniprot ID   -
Organism   Lactococcus cremoris strain W34     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 646959..657405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLW34_RS03365 (LLW34_0649) - 648899..649297 (+) 399 WP_011676775.1 hypothetical protein -
  LLW34_RS03370 (LLW34_0650) - 649403..650335 (+) 933 WP_014572202.1 ABC transporter ATP-binding protein -
  LLW34_RS03375 (LLW34_0651) - 650332..651693 (+) 1362 WP_014572203.1 ABC transporter permease -
  LLW34_RS03380 (LLW34_0652) comEA 651959..652405 (+) 447 WP_228763864.1 helix-hairpin-helix domain-containing protein Machinery gene
  LLW34_RS03385 (LLW34_0653) comEC 652533..653759 (+) 1227 WP_332375356.1 ComEC/Rec2 family competence protein Machinery gene
  LLW34_RS03390 (LLW34_0654) comEC 653800..654309 (+) 510 WP_021165649.1 MBL fold metallo-hydrolase Machinery gene
  LLW34_RS03395 (LLW34_0655) - 654386..655276 (+) 891 WP_205288343.1 IS982-like element ISLll1 family transposase -
  LLW34_RS03400 (LLW34_0656) - 655297..655602 (+) 306 Protein_654 ComEC/Rec2 family competence protein -
  LLW34_RS03405 (LLW34_0657) - 655886..656662 (+) 777 WP_014572211.1 alpha/beta hydrolase family protein -
  LLW34_RS03410 (LLW34_0658) - 656845..657060 (+) 216 WP_014572212.1 F0F1 ATP synthase subunit C -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16038.01 Da        Isoelectric Point: 4.6909

>NTDB_id=315805 LLW34_RS03380 WP_228763864.1 651959..652405(+) (comEA) [Lactococcus cremoris strain W34]
MVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPISSSVSNQVS
TTSGPKININKADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide


Download         Length: 447 bp        

>NTDB_id=315805 LLW34_RS03380 WP_228763864.1 651959..652405(+) (comEA) [Lactococcus cremoris strain W34]
ATGGTTGATTTGAAAGGAGCGGTTGCAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAG
GCAAGCGGGAGGATTTACTGACCAAGCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTAATTT
ATGTACCAAAACTTGGGGAAAGTTCAAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCA
ACGACAAGTGGTCCAAAAATAAATATCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAA
AGCGCAAGATATTATTGATTTTCGCATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTG
GGGATAAAACATTAGAAAAATTGAAAGATGAGATTTCTATTGATTAA

Domains

Predicted by InterProScan.

SLBB domain (SLBB)

(1-56)

Helix-hairpin-helix motif (HHH_3)

(84-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

96.622

100

0.966
  comEA/celA/cilE Streptococcus mitis SK321

51.02

99.324

0.507
  comEA Latilactobacillus sakei subsp. sakei 23K

44.512

100

0.493
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

48.98

99.324

0.486
  comEA/celA/cilE Streptococcus pneumoniae Rx1

48.299

99.324

0.48
  comEA/celA/cilE Streptococcus pneumoniae D39

48.299

99.324

0.48
  comEA/celA/cilE Streptococcus pneumoniae R6

48.299

99.324

0.48
  comEA/celA/cilE Streptococcus mitis NCTC 12261

47.619

99.324

0.473
  comEA Streptococcus thermophilus LMD-9

43.421

100

0.446
  comEA Staphylococcus aureus N315

41.781

98.649

0.412
  comEA Staphylococcus aureus MW2

41.781

98.649

0.412
  comEA Bacillus subtilis subsp. subtilis str. 168

40.816

99.324

0.405

Multiple sequence alignment