Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   LLW34_RS03390 Genome accession   NZ_CP032430
Coordinates   653800..654309 (+) Length   169 a.a.
NCBI ID   WP_021165649.1    Uniprot ID   -
Organism   Lactococcus cremoris strain W34     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 628100..667624 653800..654309 within 0
IS/Tn 654386..655276 653800..654309 flank 77


Gene organization within MGE regions


Location: 628100..667624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLW34_RS03265 (LLW34_0624) - 628100..628786 (+) 687 WP_014572148.1 amino acid ABC transporter permease -
  LLW34_RS03270 (LLW34_0625) - 628786..629520 (+) 735 WP_011676803.1 amino acid ABC transporter ATP-binding protein -
  LLW34_RS03275 (LLW34_0626) mecA 629649..630350 (+) 702 WP_011676802.1 adaptor protein MecA Regulator
  LLW34_RS03280 (LLW34_0627) - 630353..631678 (+) 1326 WP_011676801.1 MraY family glycosyltransferase -
  LLW34_RS03285 (LLW34_02790) - 631752..633136 (+) 1385 WP_205288033.1 IS3 family transposase -
  LLW34_RS03290 (LLW34_0631) - 633210..634357 (+) 1148 WP_095559415.1 IS3 family transposase -
  LLW34_RS03295 (LLW34_0632) sufC 634603..635373 (+) 771 WP_011676800.1 Fe-S cluster assembly ATPase SufC -
  LLW34_RS03300 (LLW34_0633) sufD 635513..636769 (+) 1257 WP_021211874.1 Fe-S cluster assembly protein SufD -
  LLW34_RS03305 (LLW34_0634) - 636769..637986 (+) 1218 WP_021211873.1 cysteine desulfurase -
  LLW34_RS03310 (LLW34_0635) - 637988..638470 (+) 483 WP_031286519.1 hypothetical protein -
  LLW34_RS03315 (LLW34_0636) sufU 638604..639062 (+) 459 WP_011676796.1 Fe-S cluster assembly sulfur transfer protein SufU -
  LLW34_RS03320 (LLW34_0637) sufB 639158..640570 (+) 1413 WP_004255207.1 Fe-S cluster assembly protein SufB -
  LLW34_RS03325 (LLW34_0638) - 640918..641208 (+) 291 WP_011676794.1 WXG100 family type VII secretion target -
  LLW34_RS03330 (LLW34_0639) - 641316..641750 (+) 435 WP_021211585.1 hypothetical protein -
  LLW34_RS03340 (LLW34_0642) tenA 643079..643735 (+) 657 WP_205288341.1 thiaminase II -
  LLW34_RS03345 (LLW34_0643) - 644123..645301 (+) 1179 WP_205288342.1 SLC13 family permease -
  LLW34_RS03350 (LLW34_0644) - 645474..646034 (+) 561 WP_011676776.1 GNAT family N-acetyltransferase -
  LLW34_RS03355 (LLW34_0646) - 646134..647281 (+) 1148 WP_095559415.1 IS3 family transposase -
  LLW34_RS03365 (LLW34_0649) - 648899..649297 (+) 399 WP_011676775.1 hypothetical protein -
  LLW34_RS03370 (LLW34_0650) - 649403..650335 (+) 933 WP_014572202.1 ABC transporter ATP-binding protein -
  LLW34_RS03375 (LLW34_0651) - 650332..651693 (+) 1362 WP_014572203.1 ABC transporter permease -
  LLW34_RS03380 (LLW34_0652) comEA 651959..652405 (+) 447 WP_228763864.1 helix-hairpin-helix domain-containing protein Machinery gene
  LLW34_RS03385 (LLW34_0653) comEC 652533..653759 (+) 1227 WP_332375356.1 ComEC/Rec2 family competence protein Machinery gene
  LLW34_RS03390 (LLW34_0654) comEC 653800..654309 (+) 510 WP_021165649.1 MBL fold metallo-hydrolase Machinery gene
  LLW34_RS03395 (LLW34_0655) - 654386..655276 (+) 891 WP_205288343.1 IS982-like element ISLll1 family transposase -
  LLW34_RS03400 (LLW34_0656) - 655297..655602 (+) 306 Protein_654 ComEC/Rec2 family competence protein -
  LLW34_RS03405 (LLW34_0657) - 655886..656662 (+) 777 WP_014572211.1 alpha/beta hydrolase family protein -
  LLW34_RS03410 (LLW34_0658) - 656845..657060 (+) 216 WP_014572212.1 F0F1 ATP synthase subunit C -
  LLW34_RS03415 (LLW34_0659) atpB 657107..657820 (+) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  LLW34_RS03420 (LLW34_0660) atpF 657835..658341 (+) 507 WP_010906128.1 F0F1 ATP synthase subunit B -
  LLW34_RS03425 (LLW34_0661) - 658343..658870 (+) 528 WP_014572213.1 F0F1 ATP synthase subunit delta -
  LLW34_RS03430 (LLW34_0662) atpA 659058..660560 (+) 1503 WP_014572214.1 F0F1 ATP synthase subunit alpha -
  LLW34_RS03435 (LLW34_0663) - 660576..661445 (+) 870 WP_011676766.1 F0F1 ATP synthase subunit gamma -
  LLW34_RS03440 (LLW34_0664) atpD 661617..663026 (+) 1410 WP_021211822.1 F0F1 ATP synthase subunit beta -
  LLW34_RS03445 (LLW34_0665) - 663211..663636 (+) 426 WP_011676764.1 F0F1 ATP synthase subunit epsilon -
  LLW34_RS03450 (LLW34_0666) - 663740..664399 (+) 660 WP_259751054.1 hypothetical protein -
  LLW34_RS03455 (LLW34_0667) - 664400..664696 (+) 297 WP_242515062.1 hypothetical protein -
  LLW34_RS03460 (LLW34_0669) - 664752..665863 (+) 1112 WP_088792927.1 IS3-like element IS981 family transposase -
  LLW34_RS03465 (LLW34_02800) - 665956..666168 (+) 213 WP_228764053.1 hypothetical protein -
  LLW34_RS03470 (LLW34_02805) - 666276..666824 (+) 549 WP_014572218.1 hypothetical protein -
  LLW34_RS03475 (LLW34_0671) - 666881..667624 (-) 744 WP_011676756.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 18923.62 Da        Isoelectric Point: 6.9787

>NTDB_id=315807 LLW34_RS03390 WP_021165649.1 653800..654309(+) (comEC) [Lactococcus cremoris strain W34]
MRASITAVDIGQGDSIFLQDKFNKENILIDTGGRLALPQKNWQKAQTQSNADKTLIPYLESMGVAHIDQLILTHTDADHV
GDFLSLADKIKIREIWISPGELTNISFVEKLKKAKIPIHVSKVGDKIPIFDSFLQVLSNGYTKKGDNNDSIVTYGNFYHT
KFLFTDPNC

Nucleotide


Download         Length: 510 bp        

>NTDB_id=315807 LLW34_RS03390 WP_021165649.1 653800..654309(+) (comEC) [Lactococcus cremoris strain W34]
ATGAGAGCAAGCATTACAGCAGTTGATATCGGACAAGGAGATAGTATTTTTCTACAAGATAAGTTTAATAAAGAAAACAT
TCTGATTGATACTGGGGGACGACTTGCTCTTCCTCAAAAAAATTGGCAAAAAGCGCAGACGCAATCAAATGCTGACAAAA
CGCTTATCCCTTATTTAGAATCCATGGGGGTGGCTCATATTGACCAACTTATTCTGACTCATACAGATGCTGACCATGTT
GGCGATTTCTTGTCCTTAGCTGATAAAATAAAAATAAGAGAAATCTGGATCAGTCCGGGAGAATTAACCAACATTAGTTT
TGTGGAAAAATTAAAAAAAGCAAAAATTCCGATTCATGTTAGTAAAGTTGGGGATAAAATTCCGATTTTTGATAGTTTTC
TTCAAGTTTTATCAAATGGTTACACTAAAAAAGGGGATAATAATGATTCCATAGTGACTTATGGAAACTTTTACCATACA
AAATTTCTTTTTACAGACCCGAATTGCTAG

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(11-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Lactococcus lactis subsp. cremoris KW2

98.788

97.633

0.964
  comEC/celB Streptococcus mitis SK321

46.707

98.817

0.462
  comEC/celB Streptococcus pneumoniae TIGR4

44.91

98.817

0.444
  comEC/celB Streptococcus pneumoniae Rx1

44.311

98.817

0.438
  comEC/celB Streptococcus pneumoniae D39

44.311

98.817

0.438
  comEC/celB Streptococcus pneumoniae R6

44.311

98.817

0.438
  comEC/celB Streptococcus mitis NCTC 12261

43.713

98.817

0.432
  comEC Latilactobacillus sakei subsp. sakei 23K

38.037

96.45

0.367

Multiple sequence alignment