Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   D3880_RS18855 Genome accession   NZ_CP032419
Coordinates   4148516..4149733 (+) Length   405 a.a.
NCBI ID   WP_119894954.1    Uniprot ID   A0A385Z6H0
Organism   Pseudomonas cavernae strain K2W31S-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4143516..4154733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3880_RS18835 (D3880_18840) - 4143918..4144739 (-) 822 WP_238474376.1 hypothetical protein -
  D3880_RS18840 (D3880_18845) - 4144760..4145569 (-) 810 WP_238474377.1 hypothetical protein -
  D3880_RS23220 (D3880_18850) pilA 4146271..4146456 (-) 186 WP_119894952.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D3880_RS18850 (D3880_18855) pilB 4146683..4148386 (+) 1704 WP_119894953.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D3880_RS18855 (D3880_18860) pilC 4148516..4149733 (+) 1218 WP_119894954.1 type II secretion system F family protein Machinery gene
  D3880_RS18860 (D3880_18865) pilD 4149736..4150608 (+) 873 WP_119894955.1 prepilin peptidase Machinery gene
  D3880_RS18865 (D3880_18870) coaE 4150612..4151229 (+) 618 WP_119894956.1 dephospho-CoA kinase -
  D3880_RS18870 (D3880_18875) yacG 4151226..4151426 (+) 201 WP_119894957.1 DNA gyrase inhibitor YacG -
  D3880_RS18875 (D3880_18880) - 4151586..4152275 (-) 690 WP_119894958.1 energy-coupling factor ABC transporter permease -
  D3880_RS18880 (D3880_18885) - 4152272..4152697 (-) 426 WP_119894959.1 GNAT family N-acetyltransferase -
  D3880_RS18885 (D3880_18890) - 4152813..4153439 (+) 627 WP_119894960.1 DUF1780 domain-containing protein -
  D3880_RS18890 (D3880_18895) - 4153436..4153930 (+) 495 WP_119894961.1 MOSC domain-containing protein -
  D3880_RS18895 (D3880_18900) - 4153972..4154145 (+) 174 WP_119895784.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44287.08 Da        Isoelectric Point: 9.8649

>NTDB_id=315708 D3880_RS18855 WP_119894954.1 4148516..4149733(+) (pilC) [Pseudomonas cavernae strain K2W31S-8]
MAEKALKTSVFTWEGTDKKGARIKGELRGQNPALIKAQLRKQGINPTKVRKRSVSLFGAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDELKQEVAAGNSFASSLRKKPEYFDDLYCNLVDAGEQSGSLEALLDRVATYKEKT
EALKAKIKKAMNYPIAVIVVAVIVTAILLIKVVPQFKAVFEGFGAELPAFTLMVVGASEFLQEWWFLFLAAIIGAVFVFK
EAHKRSEKFRNWVDRTLLKLPIVGDILYKSAIARFARTLSTTFAAGVPLVDALDSVAGATGNVVFRTATNKVKSDVSTGM
QLNFSMRTTGVFPSMAIQMTAIGEESGALDEMLSKVATFYEDEVDNMVDGLTALMEPIIMSVLGVLVGGLVIAMYLPIFQ
LGNVI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=315708 D3880_RS18855 WP_119894954.1 4148516..4149733(+) (pilC) [Pseudomonas cavernae strain K2W31S-8]
ATGGCTGAAAAAGCATTAAAAACCAGTGTGTTTACCTGGGAAGGTACAGACAAGAAGGGCGCCCGCATCAAGGGTGAACT
GAGAGGTCAAAATCCCGCCCTGATCAAGGCACAGTTGCGCAAACAGGGCATCAACCCGACCAAGGTGCGCAAGAGGTCCG
TCTCGCTGTTCGGCGCCGGCAAGAAGATCAAGCCCATGGATATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGTGTACCGCTGCTGCAGAGTTTCGACATCATCGGCGAAGGTTTCGACAACCCCAACATGCGCAAGCTGGTGGACGA
GCTCAAGCAGGAAGTGGCCGCAGGCAACAGCTTCGCCAGTTCCTTGCGCAAGAAGCCCGAATACTTCGACGATCTCTACT
GCAACCTGGTGGACGCAGGCGAGCAGTCTGGCTCCCTGGAAGCGCTGCTCGATCGCGTCGCCACCTACAAGGAGAAGACC
GAGGCGCTCAAGGCCAAGATCAAGAAGGCGATGAACTATCCGATTGCGGTGATCGTGGTGGCCGTCATCGTTACTGCCAT
TCTGTTGATCAAAGTGGTGCCACAGTTCAAGGCGGTATTCGAGGGCTTCGGGGCCGAACTGCCAGCATTTACCCTTATGG
TGGTGGGCGCTTCCGAATTCCTTCAGGAATGGTGGTTTCTTTTCTTGGCCGCCATCATTGGCGCGGTGTTTGTGTTCAAG
GAGGCACACAAACGCTCGGAGAAATTCCGCAACTGGGTAGACCGCACCCTGCTCAAACTGCCCATCGTCGGCGACATCCT
CTATAAATCGGCGATAGCCCGTTTCGCACGAACGCTCTCCACCACCTTCGCCGCCGGCGTACCGCTGGTCGATGCACTCG
ATTCGGTGGCCGGTGCCACCGGCAACGTGGTTTTCCGCACCGCCACCAACAAAGTCAAAAGCGACGTGTCGACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCGGCGTCTTTCCCTCCATGGCAATACAAATGACCGCCATCGGCGAAGAATCCGG
TGCGCTGGATGAGATGCTCAGCAAGGTAGCGACATTCTACGAGGACGAAGTGGATAACATGGTCGACGGCCTGACCGCGC
TGATGGAGCCCATAATCATGTCCGTGCTCGGCGTGCTGGTCGGCGGCCTGGTGATTGCCATGTACCTGCCCATCTTCCAA
CTCGGCAACGTTATCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A385Z6H0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

85.679

100

0.857
  pilC Acinetobacter baylyi ADP1

61.084

100

0.612
  pilC Acinetobacter baumannii D1279779

60

100

0.6
  pilC Legionella pneumophila strain ERS1305867

55.556

97.778

0.543
  pilG Neisseria meningitidis 44/76-A

45.025

99.259

0.447
  pilG Neisseria gonorrhoeae MS11

45.025

99.259

0.447
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

40.404

97.778

0.395
  pilC Thermus thermophilus HB27

36.591

98.519

0.36

Multiple sequence alignment