Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D3880_RS18850 Genome accession   NZ_CP032419
Coordinates   4146683..4148386 (+) Length   567 a.a.
NCBI ID   WP_119894953.1    Uniprot ID   A0A385Z701
Organism   Pseudomonas cavernae strain K2W31S-8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4141683..4153386
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3880_RS18825 (D3880_18830) nadC 4141943..4142791 (+) 849 WP_119894949.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D3880_RS18830 (D3880_18835) - 4143013..4143921 (-) 909 WP_119894950.1 ABC transporter ATP-binding protein -
  D3880_RS18835 (D3880_18840) - 4143918..4144739 (-) 822 WP_238474376.1 hypothetical protein -
  D3880_RS18840 (D3880_18845) - 4144760..4145569 (-) 810 WP_238474377.1 hypothetical protein -
  D3880_RS23220 (D3880_18850) pilA 4146271..4146456 (-) 186 WP_119894952.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D3880_RS18850 (D3880_18855) pilB 4146683..4148386 (+) 1704 WP_119894953.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D3880_RS18855 (D3880_18860) pilC 4148516..4149733 (+) 1218 WP_119894954.1 type II secretion system F family protein Machinery gene
  D3880_RS18860 (D3880_18865) pilD 4149736..4150608 (+) 873 WP_119894955.1 prepilin peptidase Machinery gene
  D3880_RS18865 (D3880_18870) coaE 4150612..4151229 (+) 618 WP_119894956.1 dephospho-CoA kinase -
  D3880_RS18870 (D3880_18875) yacG 4151226..4151426 (+) 201 WP_119894957.1 DNA gyrase inhibitor YacG -
  D3880_RS18875 (D3880_18880) - 4151586..4152275 (-) 690 WP_119894958.1 energy-coupling factor ABC transporter permease -
  D3880_RS18880 (D3880_18885) - 4152272..4152697 (-) 426 WP_119894959.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62551.64 Da        Isoelectric Point: 5.5846

>NTDB_id=315707 D3880_RS18850 WP_119894953.1 4146683..4148386(+) (pilB) [Pseudomonas cavernae strain K2W31S-8]
MNDSVSLSGLARQMVLAELLDEKSAQQAQQQAQRNKLPLVTYLVQNKLVKSRALAELAAEQFGVAFVDLNCIDKEGQPRE
LVSEKLIRQHRALPLWRRGNKLFVALSDPSNHQAVTDIQFSTGLTTEAILVEDDKLGDAIEKFFETANSGLEELADVDLD
GLDIEAVDDDKQDSVAGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGILHEVARPPIQLAPRISAR
LKVMAALDISERRRPQDGRIKMKVSKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEENQKDLYMAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTIDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCSHCKKAVQIPHEALLHEGFP
EDKIGSFKLYGPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDIAAQMRQDGFNDLRASGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=315707 D3880_RS18850 WP_119894953.1 4146683..4148386(+) (pilB) [Pseudomonas cavernae strain K2W31S-8]
ATGAACGACAGCGTCTCTCTCTCAGGCCTGGCCCGGCAGATGGTGCTGGCCGAATTGCTGGATGAAAAAAGCGCACAGCA
AGCCCAGCAGCAGGCCCAGCGCAACAAGCTGCCGCTGGTCACCTACCTGGTGCAGAACAAGCTGGTGAAGAGCCGTGCCC
TGGCCGAACTGGCCGCCGAGCAGTTCGGCGTGGCCTTCGTCGACCTCAACTGCATTGATAAAGAGGGGCAGCCCAGGGAA
CTTGTCAGTGAGAAGCTGATCCGCCAACACCGCGCGCTGCCACTCTGGCGGCGCGGCAACAAGCTGTTCGTCGCCCTGTC
CGACCCCAGCAATCACCAAGCGGTCACCGATATCCAGTTCAGCACCGGGCTGACCACCGAAGCCATCCTGGTGGAGGACG
ACAAGCTGGGCGACGCCATCGAGAAATTTTTCGAGACCGCCAACAGCGGCCTGGAGGAACTCGCCGATGTCGATCTGGAC
GGCCTGGATATCGAAGCGGTCGATGATGACAAGCAAGACAGTGTCGCCGGTCAGGACGCAGACGACGCTCCGGTGGTGCG
CTTCGTCAACAAGATGCTGCTGGACGCCATCAAGGGCGGCTCGTCCGACCTGCACTTCGAGCCCTACGAGAAGGCCTATC
GTGTCCGCTTTCGCACCGACGGAATCCTCCACGAAGTGGCCCGCCCGCCAATCCAGCTGGCACCGCGCATCTCCGCCCGC
CTCAAGGTGATGGCGGCGCTGGACATCTCCGAGCGGCGCAGGCCGCAGGACGGCCGGATCAAGATGAAGGTGTCGAAGAC
CAAGGCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCCACCA
GCGCGCAGATGGGTATCGATGCACTGGGTTACGAGGAAAACCAGAAAGACCTGTACATGGCCGCTCTCAAGCAACCGCAG
GGCATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACCGTGTCGCTTTATACCGGCCTGAATATTCTCAACACGAT
CGACGTGAACATCTCCACCGCCGAAGACCCGGTGGAAATCAACCTGGAGGGGATCAATCAGGTCAACGTCAACCCCAAGC
AGGGCATGGACTTCTCCCAGGCCCTGCGCGCCTTCCTGCGCCAAGACCCGGACATCATCATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCGGAAATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGATGTCGACCCTGCACACCAACAGCGCCGC
GGAGACCCTGACCCGCCTGCGCAATATGGGGGTGCCGTCATTCAACATCGCCACCTCGGTCAACCTGATCATCGCCCAGC
GTCTGGCGCGCAAGCTGTGCAGCCACTGCAAGAAGGCCGTCCAGATCCCTCACGAAGCACTGCTTCACGAGGGCTTCCCG
GAAGACAAGATCGGCAGCTTCAAGCTCTATGGCCCGGTCGGCTGCGAGAATTGCAAGGGTGGCTACAAGGGCCGAGTCGG
GATTTATGAAGTGGTTAAAAACACCCCGGCGCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGATATTGCCGCGC
AGATGCGCCAGGATGGCTTCAACGACTTGCGCGCATCCGGTTTGCTGAAAGCCATGCAAGGCATCACCAGCCTCGAAGAG
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A385Z701

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.298

100

0.596
  pilB Acinetobacter baylyi ADP1

59.083

100

0.591
  pilB Legionella pneumophila strain ERS1305867

57.774

99.824

0.577
  pilB Vibrio parahaemolyticus RIMD 2210633

50.352

100

0.504
  pilB Vibrio cholerae strain A1552

50.814

97.531

0.496
  pilF Neisseria gonorrhoeae MS11

49.381

99.647

0.492
  pilB Vibrio campbellii strain DS40M4

48.759

99.471

0.485
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.301

93.651

0.377
  pilF Thermus thermophilus HB27

37.522

99.647

0.374

Multiple sequence alignment