Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   E4T63_RS23765 Genome accession   NZ_CP038438
Coordinates   5158798..5159238 (-) Length   146 a.a.
NCBI ID   WP_098965660.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain LBUM677     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5153798..5164238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T63_RS23750 (E4T63_23750) - 5154631..5157579 (+) 2949 WP_135296583.1 DUF748 domain-containing protein -
  E4T63_RS23755 (E4T63_23755) - 5157579..5157875 (+) 297 WP_115988878.1 DUF2845 domain-containing protein -
  E4T63_RS23760 (E4T63_23760) - 5158142..5158498 (-) 357 WP_025108814.1 BON domain-containing protein -
  E4T63_RS23765 (E4T63_23765) pilA/pilAI 5158798..5159238 (-) 441 WP_098965660.1 pilin Machinery gene
  E4T63_RS23770 (E4T63_23770) pilB 5159467..5161167 (+) 1701 WP_097089222.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E4T63_RS23775 (E4T63_23775) pilC 5161170..5162387 (+) 1218 WP_098965663.1 type II secretion system F family protein Machinery gene
  E4T63_RS23780 (E4T63_23780) pilD 5162389..5163261 (+) 873 WP_096795219.1 A24 family peptidase Machinery gene
  E4T63_RS23785 (E4T63_23785) coaE 5163258..5163881 (+) 624 WP_135296584.1 dephospho-CoA kinase -
  E4T63_RS23790 (E4T63_23790) yacG 5163878..5164075 (+) 198 WP_003228296.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14525.60 Da        Isoelectric Point: 8.1087

>NTDB_id=314915 E4T63_RS23765 WP_098965660.1 5158798..5159238(-) (pilA/pilAI) [Pseudomonas fluorescens strain LBUM677]
MNNQKGFTLIELLIVVAIIGILATIALPQYTKYQARSKVTAGLAEISALKVPFEDTINQGTDPTLTLVAGSASFTTTNCG
TVTASGTASTGAGTIVCTLANAPAPVLGKTITLTRSSGTGSAAGVWSCASTVNADYAPKGCPGSGA

Nucleotide


Download         Length: 441 bp        

>NTDB_id=314915 E4T63_RS23765 WP_098965660.1 5158798..5159238(-) (pilA/pilAI) [Pseudomonas fluorescens strain LBUM677]
ATGAACAACCAAAAAGGTTTCACTCTGATCGAGCTGCTGATCGTGGTAGCGATCATCGGGATTCTGGCGACGATTGCCCT
GCCGCAATACACCAAGTATCAGGCACGCTCGAAAGTCACTGCCGGGCTGGCGGAAATCAGCGCGCTAAAAGTGCCTTTCG
AGGACACCATTAATCAAGGTACGGACCCGACGCTGACGCTGGTGGCAGGTAGCGCGTCGTTTACTACCACCAATTGCGGC
ACCGTGACCGCGTCCGGCACGGCGTCGACTGGCGCGGGGACCATCGTCTGCACCTTGGCCAACGCCCCGGCTCCAGTGCT
GGGCAAAACCATCACGCTGACTCGTTCTTCGGGGACCGGCTCGGCGGCGGGTGTCTGGAGCTGCGCTTCGACGGTGAACG
CCGACTACGCGCCTAAGGGTTGCCCAGGCAGCGGTGCCTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.575

100

0.466
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.667

100

0.459
  pilA Vibrio cholerae strain A1552

44.667

100

0.459
  pilA Vibrio cholerae C6706

44.667

100

0.459
  pilA Acinetobacter baumannii strain A118

47.183

97.26

0.459
  comP Acinetobacter baylyi ADP1

41.509

100

0.452
  pilA Pseudomonas aeruginosa PAK

40.645

100

0.432
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.366

97.26

0.432
  pilA2 Legionella pneumophila str. Paris

37.333

100

0.384
  pilA2 Legionella pneumophila strain ERS1305867

36.667

100

0.377
  pilA Vibrio parahaemolyticus RIMD 2210633

38.298

96.575

0.37