Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CDG62_RS04190 Genome accession   NZ_CP032286
Coordinates   304411..306489 (+) Length   692 a.a.
NCBI ID   WP_087527297.1    Uniprot ID   A0A385N729
Organism   Acinetobacter sp. WCHA55     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 299411..311489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG62_RS04170 (CDG62_04175) pilM 301417..302475 (+) 1059 WP_087527293.1 pilus assembly protein PilM Machinery gene
  CDG62_RS04175 (CDG62_04180) pilN 302475..303131 (+) 657 WP_087527294.1 PilN domain-containing protein Machinery gene
  CDG62_RS04180 (CDG62_04185) pilO 303128..303865 (+) 738 WP_087527295.1 type 4a pilus biogenesis protein PilO Machinery gene
  CDG62_RS04185 (CDG62_04190) pilP 303865..304392 (+) 528 WP_087527296.1 pilus assembly protein PilP Machinery gene
  CDG62_RS04190 (CDG62_04195) pilQ 304411..306489 (+) 2079 WP_087527297.1 type IV pilus secretin PilQ Machinery gene
  CDG62_RS04195 (CDG62_04200) - 306578..307060 (+) 483 WP_265088291.1 shikimate kinase -
  CDG62_RS04200 (CDG62_04205) aroB 307091..308176 (+) 1086 WP_087527299.1 3-dehydroquinate synthase -
  CDG62_RS04205 (CDG62_04210) - 308184..309023 (+) 840 WP_087527300.1 hypothetical protein -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75173.37 Da        Isoelectric Point: 5.2441

>NTDB_id=314733 CDG62_RS04190 WP_087527297.1 304411..306489(+) (pilQ) [Acinetobacter sp. WCHA55]
MKNSAKDFIFGDGNTMNHMFRQFSMGAIAMAVMQAASAQVSITNIVPMQIPGQGTEIRVMFNGLPPQPQAYQLEQPSRLV
LDFDKAQQSLKQGSIAVATAEASTVDVTSDAQRSRLTVNLADAGAFTTRVEGNTFILKINSASPTAVTPVAAARSEQGIS
NIGFQRGSDGEGQVVIDLKGSNTPVDVKQQGSKIVVRVLGNKIPAHLSRRLNVNDFATPVSTIDAQNDGSNGLITIQSNG
SFEYMAYQAEDKLTISLKRPEDKNPMKPKAPSAYTGKKISLDFQDIEVRRVLQLLADFTGVNMVASDTVQGNITLRLKDV
PWDQALDIIMKTKNLDKRRNGNVIWIAPVAELIKSEEDEAKAIAQSIKLAPIQTDYIQLSYAKSADVLKLLEDSRDSKGS
VSNRTAGSESLALESLLSSRGSAVSDSRTNTLIINDTQQNIDKIRRMIDLLDVAVKQVMVEARIVTASTDFSRELGIKWS
AKKLGNSPVWGGDFQPSFDLGVDLATNTAGTFNFGLLKISDYMLNLELSALQADGHGEVLSAPKVLTGDKQKAYILRGTE
IPYQTWSATEGLKTEFRAANLKLEVTPSITPDGKVQMALMVEKDSIGDITNDGIAIDTSELQTNVLVDDGETVVLGGIFD
DTKRNDYQKVPFLGDLPVVGNLFKNNVKTTIQTELLIFVTPRIVNDSVSRNH

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=314733 CDG62_RS04190 WP_087527297.1 304411..306489(+) (pilQ) [Acinetobacter sp. WCHA55]
ATGAAAAATAGTGCTAAAGATTTTATTTTTGGGGATGGCAATACGATGAACCATATGTTCCGTCAGTTTTCAATGGGTGC
AATTGCAATGGCGGTAATGCAGGCTGCAAGTGCACAAGTCAGTATTACGAATATTGTGCCGATGCAAATTCCAGGACAGG
GAACAGAAATTCGGGTCATGTTCAACGGTTTGCCACCTCAACCGCAAGCCTATCAGTTGGAACAGCCATCACGTCTGGTT
TTAGATTTCGATAAAGCACAGCAGAGCTTAAAACAAGGCAGTATTGCAGTTGCAACGGCTGAAGCCAGTACTGTGGATGT
GACTTCAGATGCACAACGTTCACGTTTAACTGTAAATTTGGCGGATGCAGGTGCATTTACTACACGTGTTGAAGGTAATA
CTTTTATTCTGAAAATTAACTCAGCGAGCCCAACAGCAGTAACACCTGTTGCTGCGGCACGTTCAGAGCAAGGGATTTCA
AATATTGGGTTCCAGCGTGGTTCAGATGGAGAAGGCCAAGTTGTCATTGATTTAAAAGGCAGTAATACCCCTGTCGATGT
AAAACAGCAAGGTAGTAAAATTGTAGTGCGCGTTTTGGGGAATAAAATTCCTGCACATTTATCACGTCGTCTCAATGTGA
ATGATTTCGCAACCCCTGTATCGACCATTGATGCACAAAACGATGGTAGTAATGGTCTGATCACAATTCAATCGAATGGT
AGTTTTGAATATATGGCTTATCAGGCAGAAGATAAGCTCACCATTAGTTTGAAGCGTCCTGAAGATAAAAATCCAATGAA
GCCTAAAGCACCAAGTGCTTATACGGGCAAAAAGATTTCTTTAGATTTTCAGGATATTGAAGTGCGTCGTGTATTACAGC
TGTTGGCTGATTTTACAGGCGTTAACATGGTGGCTTCGGACACGGTACAAGGTAATATTACCTTACGCCTTAAAGATGTC
CCGTGGGATCAGGCGCTTGATATTATTATGAAAACCAAGAATTTGGATAAGCGTCGCAATGGTAATGTGATTTGGATTGC
CCCTGTAGCAGAATTGATTAAATCTGAGGAAGATGAAGCCAAAGCCATCGCACAAAGCATTAAACTTGCACCGATTCAAA
CCGATTATATTCAATTGAGTTATGCAAAATCGGCAGATGTATTGAAATTGTTAGAAGACTCTCGTGATAGTAAGGGGAGT
GTGTCGAATCGTACAGCGGGTTCAGAGTCATTGGCTTTAGAAAGCTTATTGAGTTCACGGGGGAGTGCGGTTTCTGATTC
ACGTACCAATACCTTAATTATTAATGATACACAGCAAAATATTGACAAGATCCGCCGCATGATTGACTTGTTGGATGTCG
CAGTGAAACAGGTGATGGTCGAAGCCCGTATCGTCACAGCATCAACAGACTTTTCTCGTGAATTGGGGATTAAGTGGAGT
GCGAAGAAGCTGGGTAATAGCCCAGTCTGGGGCGGAGATTTCCAGCCAAGTTTTGATTTGGGTGTAGATTTGGCAACAAA
TACTGCGGGTACATTTAACTTTGGTTTATTGAAAATTTCAGACTATATGTTGAACCTTGAGTTAAGTGCACTTCAAGCGG
ATGGTCATGGTGAAGTGTTATCAGCGCCTAAAGTACTTACTGGCGATAAACAGAAAGCATATATCTTGCGTGGTACAGAG
ATTCCCTATCAAACATGGAGTGCGACAGAAGGTTTAAAAACCGAGTTTCGCGCGGCTAACTTAAAGTTAGAAGTGACGCC
AAGTATTACTCCAGATGGTAAAGTTCAAATGGCGCTCATGGTTGAGAAAGACTCAATTGGTGACATTACCAATGATGGTA
TTGCGATTGACACCAGCGAATTACAAACCAATGTGTTGGTAGATGATGGTGAAACTGTCGTATTGGGTGGTATTTTTGAT
GATACGAAGCGTAATGACTATCAAAAAGTGCCATTCTTGGGTGATTTACCTGTGGTAGGTAATTTATTTAAGAATAATGT
CAAAACGACGATACAAACGGAGTTATTGATTTTTGTGACACCACGAATTGTTAATGACAGTGTTTCAAGAAATCATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(304-348)

AMIN domain (AMIN)

(55-135)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-457)

Bacterial type II and III secretion system protein (Secretin)

(530-684)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A385N729

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

64.958

100

0.678
  pilQ Acinetobacter baumannii strain A118

64.82

100

0.676
  comQ Acinetobacter baylyi ADP1

63.01

100

0.647
  pilQ Pseudomonas aeruginosa PAK

39.017

100

0.39
  pilQ Legionella pneumophila strain Lp02

37.446

99.566

0.373
  pilQ Neisseria gonorrhoeae strain FA1090

35.457

100

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.722

97.688

0.368

Multiple sequence alignment