Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CDG62_RS04180 Genome accession   NZ_CP032286
Coordinates   303128..303865 (+) Length   245 a.a.
NCBI ID   WP_087527295.1    Uniprot ID   A0A385N4U5
Organism   Acinetobacter sp. WCHA55     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 298128..308865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG62_RS04165 (CDG62_04170) ponA 298723..301251 (-) 2529 WP_087527292.1 penicillin-binding protein PBP1a -
  CDG62_RS04170 (CDG62_04175) pilM 301417..302475 (+) 1059 WP_087527293.1 pilus assembly protein PilM Machinery gene
  CDG62_RS04175 (CDG62_04180) pilN 302475..303131 (+) 657 WP_087527294.1 PilN domain-containing protein Machinery gene
  CDG62_RS04180 (CDG62_04185) pilO 303128..303865 (+) 738 WP_087527295.1 type 4a pilus biogenesis protein PilO Machinery gene
  CDG62_RS04185 (CDG62_04190) pilP 303865..304392 (+) 528 WP_087527296.1 pilus assembly protein PilP Machinery gene
  CDG62_RS04190 (CDG62_04195) pilQ 304411..306489 (+) 2079 WP_087527297.1 type IV pilus secretin PilQ Machinery gene
  CDG62_RS04195 (CDG62_04200) - 306578..307060 (+) 483 WP_265088291.1 shikimate kinase -
  CDG62_RS04200 (CDG62_04205) aroB 307091..308176 (+) 1086 WP_087527299.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27677.20 Da        Isoelectric Point: 5.1834

>NTDB_id=314731 CDG62_RS04180 WP_087527295.1 303128..303865(+) (pilO) [Acinetobacter sp. WCHA55]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNDKKTDIPQISYSVKAKTYRYVGAAATTDVDVKDTKNDKKAD
AGGAK

Nucleotide


Download         Length: 738 bp        

>NTDB_id=314731 CDG62_RS04180 WP_087527295.1 303128..303865(+) (pilO) [Acinetobacter sp. WCHA55]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAACTAAGAAAAAAATGACAGTTGATAAGTTTTT
TCAACAGTTTAATACTTTAGATCCAAACAATTACGGTAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTTTAGTGATTGGGGCATTGGGTTATTTTGTCGTGATTAAGCCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAACTTGCAGCAATATCAAGCACAGTTGCAAGAAAT
GGAAGCGAATTTCAATCAGCAGTTAGAACAGTTGCCGAAAGAAACTGAAATTCCGGGTTTGGTTGAAGATATCAACGTGA
GTGGCGTAAATTCTGGATTAAAGTTTAAGAATATTCGTTTAGAGCCAGAAATTCGACAAGAGTTCTTTATCGAACAGCCG
ATTTCTATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTGCCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAACAATGATAAAAAGACAGATATTCCACAAATTTCATACAGTGTAAAAG
CGAAAACCTACCGTTATGTCGGGGCGGCAGCTACAACGGATGTTGATGTAAAAGATACAAAAAATGATAAGAAAGCCGAT
GCGGGAGGTGCCAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A385N4U5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

74.009

92.653

0.686
  comO Acinetobacter baylyi ADP1

72.072

90.612

0.653

Multiple sequence alignment