Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BEN74_RS02415 Genome accession   NZ_CP032279
Coordinates   225788..226963 (-) Length   391 a.a.
NCBI ID   WP_068910167.1    Uniprot ID   A0A3B7QB65
Organism   Acinetobacter sp. WCHAc010034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 220788..231963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN74_RS02395 (BEN74_02390) - 221820..222269 (+) 450 WP_068910208.1 MaoC family dehydratase -
  BEN74_RS02400 (BEN74_02395) - 222419..223447 (+) 1029 WP_068910171.1 AraC family transcriptional regulator -
  BEN74_RS02405 (BEN74_02400) gcvH 223532..223906 (+) 375 WP_068910206.1 glycine cleavage system protein GcvH -
  BEN74_RS02410 (BEN74_02405) - 224433..225605 (+) 1173 WP_068910169.1 MFS transporter -
  BEN74_RS02415 (BEN74_02410) pilU 225788..226963 (-) 1176 WP_068910167.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BEN74_RS02420 (BEN74_02415) - 227249..228304 (-) 1056 WP_068910165.1 3-deoxy-7-phosphoheptulonate synthase -
  BEN74_RS02425 (BEN74_02420) - 229007..230590 (+) 1584 WP_068910162.1 ATP-binding cassette domain-containing protein -
  BEN74_RS02430 (BEN74_02425) - 230692..231846 (-) 1155 WP_068910160.1 fatty acid desaturase family protein -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44171.70 Da        Isoelectric Point: 6.7842

>NTDB_id=314690 BEN74_RS02415 WP_068910167.1 225788..226963(-) (pilU) [Acinetobacter sp. WCHAc010034]
MYTAELLEDARRLMFHMLTKVVEYGGSDLFITADFPPSIKHQGLMKAFGQQALTADKTKLFAYSLMNEKQRKEFETELEC
NFAISVPNVSRFRVNVFQQQLHTGMVIRTITNEIPNFVQLKLPESLKSVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
HSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNMKAVISQRLIRTEDGKGRRAAVEIMLNTPLISDLILKGDFHELKAIMGKS
RELGMQTFDQALFELYDDGAISYEEALRNADSANELRLQIKLKSRRANPLLQSAASDFSMLPEQKAEEEAV

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=314690 BEN74_RS02415 WP_068910167.1 225788..226963(-) (pilU) [Acinetobacter sp. WCHAc010034]
ATGTACACAGCCGAATTGCTGGAAGATGCGCGCCGCTTAATGTTCCATATGCTGACTAAGGTCGTGGAATACGGCGGTTC
TGATTTATTCATTACCGCAGATTTTCCGCCCAGCATCAAGCATCAGGGCCTAATGAAAGCTTTTGGCCAGCAGGCGCTGA
CTGCAGACAAAACCAAATTATTCGCCTATAGCCTGATGAATGAAAAGCAGCGCAAGGAATTTGAAACCGAGCTGGAATGC
AACTTCGCCATCAGCGTGCCGAATGTTTCGCGCTTCCGCGTCAATGTGTTCCAGCAGCAGCTGCATACCGGCATGGTGAT
CCGCACCATCACCAATGAAATTCCGAATTTTGTCCAGCTTAAGCTGCCGGAATCGCTGAAAAGCGTGATTATGGAAAAGC
GCGGGCTGGTGCTGGTTGTCGGCGGCACAGGCTCGGGGAAATCCACCTCTTTGGCGGCCATGATTGACCACCGCAATGAG
CATTCCGCAGGGCATATTATTACCGTGGAAGATCCGGTGGAATATGTGCATAAGCATAAAAAATCCATGATTACCCACCG
CGAAGTCGGGGTGGACTGCCATTCCTGGCACAATGCCTTGAAAAATACCCTGCGTCAGGCGCCGGACGTGATTCTGATTG
GCGAGATCCGCGACAGGGAAACCATGGAGCATGCCATCGCATTCGCCGAAACCGGCCATTTGTGCTTAGGCACGCTGCAT
GCCAATAACGCCAATCAGACTTTAGACCGCATCATCAACTTTTTCCCGGAAGAGCGCCGCAATCAGCTGCTGATGGATTT
ATCATCCAATATGAAAGCAGTGATTTCACAGCGCCTGATCCGCACCGAAGACGGCAAAGGCCGGCGCGCGGCGGTGGAAA
TCATGCTGAATACGCCGCTGATCTCTGACTTGATTTTAAAAGGCGATTTTCATGAGCTGAAAGCCATTATGGGGAAATCG
CGCGAGCTGGGCATGCAGACTTTTGATCAGGCCTTGTTCGAGCTGTATGATGACGGCGCAATTTCCTATGAAGAAGCCTT
GCGCAACGCGGATTCAGCCAATGAGCTGCGCCTGCAGATCAAGCTCAAGAGCCGCCGCGCCAACCCGCTGCTGCAAAGCG
CAGCTTCAGACTTCAGCATGCTGCCGGAGCAGAAGGCTGAAGAAGAAGCTGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(91-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7QB65

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.692

93.095

0.537
  pilU Acinetobacter baylyi ADP1

56.62

90.793

0.514
  pilU Vibrio cholerae strain A1552

54.335

88.491

0.481
  pilT Acinetobacter baumannii D1279779

43.636

84.399

0.368
  pilT Acinetobacter nosocomialis M2

43.636

84.399

0.368
  pilT Acinetobacter baumannii strain A118

43.636

84.399

0.368
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.059

86.957

0.366
  pilT Pseudomonas aeruginosa PAK

43.865

83.376

0.366
  pilT Acinetobacter baylyi ADP1

43.333

84.399

0.366
  pilT Legionella pneumophila strain ERS1305867

42.727

84.399

0.361
  pilT Legionella pneumophila strain Lp02

42.727

84.399

0.361

Multiple sequence alignment