Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   DRA66_RS10365 Genome accession   NZ_CP030825
Coordinates   2067943..2068389 (-) Length   148 a.a.
NCBI ID   WP_002220239.1    Uniprot ID   A0A0Y6S569
Organism   Neisseria meningitidis strain 12-208     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2062943..2073389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DRA66_RS10350 (DRA66_12210) - 2063659..2065695 (+) 2037 WP_002247939.1 M3 family metallopeptidase -
  DRA66_RS11275 - 2065712..2065843 (+) 132 WP_257623167.1 hypothetical protein -
  DRA66_RS10355 (DRA66_12215) - 2065959..2066525 (-) 567 WP_002236626.1 manganese efflux pump MntP family protein -
  DRA66_RS10360 (DRA66_12220) - 2066989..2067489 (-) 501 WP_002220237.1 REP-associated tyrosine transposase -
  DRA66_RS10365 (DRA66_12230) pilE 2067943..2068389 (-) 447 WP_002220239.1 pilin Machinery gene
  DRA66_RS11180 - 2068647..2068796 (-) 150 WP_227718351.1 hypothetical protein -
  DRA66_RS11280 - 2068801..2068929 (+) 129 WP_256864603.1 hypothetical protein -
  DRA66_RS10375 - 2069053..2069250 (+) 198 WP_153308414.1 hypothetical protein -
  DRA66_RS10380 - 2069337..2069474 (-) 138 WP_153312121.1 hypothetical protein -
  DRA66_RS10385 (DRA66_12260) katA 2069550..2071064 (-) 1515 WP_002221625.1 catalase KatA -
  DRA66_RS10390 (DRA66_12265) - 2071412..2072263 (+) 852 WP_002248210.1 sigma-54 factor, Activator interacting domain family protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15648.94 Da        Isoelectric Point: 9.3582

>NTDB_id=301745 DRA66_RS10365 WP_002220239.1 2067943..2068389(-) (pilE) [Neisseria meningitidis strain 12-208]
MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEALTLAEGQKSAVVEYYSDNGTFPNSNTSAGIAASNEIK
GKYVASVKVEGNASVASITATMNSSNVNKDIKGKTLVLVGKQNSGSFSWECKKGSVDEKFLPSTCRTK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=301745 DRA66_RS10365 WP_002220239.1 2067943..2068389(-) (pilE) [Neisseria meningitidis strain 12-208]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTCATCGCCATCGTCGGTATCTTGGCAGCCGTCGC
CCTGCCCGCATACCAAGACTACACCGCGCGCGCCCAAATGTCCGAAGCCCTGACTTTGGCAGAAGGTCAAAAATCCGCAG
TGGTCGAGTATTATTCCGACAACGGCACATTCCCGAACAGCAATACTTCCGCAGGTATTGCTGCCTCTAACGAGATTAAA
GGTAAGTATGTGGCATCGGTTAAGGTTGAAGGTAATGCCTCTGTTGCTTCTATTACCGCTACCATGAACTCTAGTAATGT
GAATAAGGACATCAAAGGTAAAACCTTGGTACTCGTCGGCAAACAAAACTCCGGCTCGTTCTCTTGGGAATGTAAAAAAG
GTTCTGTGGACGAAAAATTCTTGCCATCTACCTGCCGCACCAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Y6S569

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

58.788

100

0.655
  pilE Neisseria gonorrhoeae strain FA1090

56.173

100

0.615
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.766

100

0.459
  pilA Ralstonia pseudosolanacearum GMI1000

38.596

100

0.446
  pilA/pilA1 Eikenella corrodens VA1

41.026

100

0.432
  comP Acinetobacter baylyi ADP1

42.384

100

0.432
  pilA2 Legionella pneumophila str. Paris

42.657

96.622

0.412
  pilA2 Legionella pneumophila strain ERS1305867

41.259

96.622

0.399
  pilA Pseudomonas aeruginosa PAK

37.255

100

0.385
  pilA Vibrio campbellii strain DS40M4

38.255

100

0.385
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.986

98.649

0.365

Multiple sequence alignment