Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C1S65_RS25245 Genome accession   NZ_CP030750
Coordinates   5543655..5544893 (-) Length   412 a.a.
NCBI ID   WP_112899630.1    Uniprot ID   A0AAD0PGR9
Organism   Pseudomonas putida strain NX-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5538655..5549893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1S65_RS25230 (C1S65_25230) - 5540323..5541921 (-) 1599 WP_112899415.1 AAA family ATPase -
  C1S65_RS25235 (C1S65_25235) aroB 5541937..5543034 (-) 1098 WP_054891191.1 3-dehydroquinate synthase -
  C1S65_RS25240 (C1S65_25240) aroK 5543132..5543650 (-) 519 WP_063541601.1 shikimate kinase AroK -
  C1S65_RS25245 (C1S65_25245) pilQ 5543655..5544893 (-) 1239 WP_112899630.1 secretin N-terminal domain-containing protein Machinery gene
  C1S65_RS25250 (C1S65_25250) - 5544847..5545857 (-) 1011 WP_112899416.1 pilus assembly protein PilP -
  C1S65_RS25255 (C1S65_25255) - 5545860..5546393 (-) 534 WP_112899417.1 PilN domain-containing protein -
  C1S65_RS25260 (C1S65_25260) pilM 5546390..5547274 (-) 885 WP_112899418.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44620.98 Da        Isoelectric Point: 4.8014

>NTDB_id=301264 C1S65_RS25245 WP_112899630.1 5543655..5544893(-) (pilQ) [Pseudomonas putida strain NX-1]
MLLTLRAMAEPLYKGEPLSLNFQDVEVRAVLQVLADYTGINLVASDTVQGNITLRLHEVPWDQALELVLRSRGLAKREEG
NVLLVAPAAELAEQSREARIDQALEAQLQPLHRELLPIHHADASDLAKLLLESLADDEPAAGRGSLSVDSRTNTLIATQS
AARLAELRQLVADLDVPVRQVMIEARIVEANVDYENVLGIRWGAQLGGMPARSGSELFVDLGAQGATSGVGVGVVRGDVL
LDLELSAMEKSGNGEVISQPKVVTADKETARILKGTEVPYQQSSKSGATSVAFREASLSLEVTPQITPDGKVIMAVRVTK
DEPDYVNALNDVPPIRKNEVNAKVRVADGETIVIGGVYSTSHNNVVDKVPFLGDLPYVGRLFRRDVLQEKKSELLVFLTP
RIMSDRAIAVSR

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=301264 C1S65_RS25245 WP_112899630.1 5543655..5544893(-) (pilQ) [Pseudomonas putida strain NX-1]
ATGCTGCTAACGCTACGGGCAATGGCGGAGCCCCTGTACAAAGGTGAGCCGCTTTCGCTGAATTTCCAGGACGTCGAGGT
TCGTGCAGTGCTGCAGGTGCTCGCCGATTATACCGGCATCAACCTGGTGGCCAGTGACACGGTTCAGGGCAATATCACCC
TGCGGCTGCACGAAGTGCCCTGGGATCAGGCATTGGAGCTGGTGTTGCGCAGCCGCGGGCTGGCCAAGCGTGAGGAGGGC
AATGTGCTGTTGGTCGCCCCCGCCGCAGAGCTCGCCGAGCAGTCGCGCGAGGCGCGCATCGACCAGGCGCTCGAGGCGCA
GTTGCAGCCGCTGCATCGGGAGTTGCTGCCGATTCATCATGCCGATGCGTCCGACCTGGCGAAACTATTGTTGGAGAGCC
TCGCGGATGACGAACCTGCGGCAGGGCGCGGCAGTTTGAGCGTGGATTCGCGGACCAACACCCTGATCGCCACCCAGTCG
GCCGCGCGCCTGGCTGAGTTGCGGCAACTGGTTGCGGATCTGGATGTGCCGGTGCGCCAGGTGATGATCGAGGCCCGGAT
CGTCGAGGCCAACGTCGATTACGAGAATGTGCTCGGTATTCGCTGGGGCGCACAGCTGGGCGGTATGCCAGCGCGGTCAG
GCAGTGAGCTGTTTGTCGATCTTGGCGCGCAGGGGGCGACCTCTGGCGTGGGCGTAGGGGTGGTGCGCGGCGATGTGCTG
CTTGATCTGGAGCTCAGTGCCATGGAGAAAAGCGGCAATGGCGAGGTCATCTCCCAGCCCAAGGTGGTCACTGCCGACAA
GGAAACCGCGCGTATTCTCAAGGGCACCGAAGTGCCGTACCAGCAGTCGAGCAAGAGCGGCGCCACCTCGGTGGCTTTCC
GCGAGGCATCGCTGTCACTGGAGGTCACGCCGCAGATCACCCCGGATGGCAAGGTCATCATGGCGGTGCGGGTGACCAAG
GACGAGCCAGACTACGTCAATGCCCTCAACGACGTGCCACCGATCCGCAAGAACGAGGTCAATGCCAAGGTGCGCGTGGC
CGATGGCGAGACGATTGTGATTGGTGGCGTGTACTCGACGTCGCATAACAATGTAGTGGACAAGGTGCCATTTCTTGGCG
ATCTGCCGTATGTTGGACGGCTGTTTCGACGCGATGTATTACAAGAGAAAAAATCTGAGCTGCTGGTCTTCCTGACTCCG
CGTATCATGAGTGACCGGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(41-87)

Bacterial type II/III secretion system short domain (Secretin_N)

(115-180)

Bacterial type II and III secretion system protein (Secretin)

(247-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.945

100

0.624
  comQ Acinetobacter baylyi ADP1

43.111

100

0.471
  pilQ Acinetobacter baumannii strain A118

41.522

100

0.464
  pilQ Acinetobacter baumannii D1279779

41.522

100

0.464
  pilQ Vibrio campbellii strain DS40M4

41.259

100

0.43
  pilQ Legionella pneumophila strain Lp02

41.844

100

0.43
  pilQ Legionella pneumophila strain ERS1305867

41.844

100

0.43
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.346

100

0.417
  pilQ Vibrio cholerae strain A1552

41.346

100

0.417
  pilQ Neisseria gonorrhoeae strain FA1090

35.831

100

0.371
  pilQ Neisseria meningitidis M1080

35.545

100

0.364
  pilQ Neisseria meningitidis MC58

35.545

100

0.364

Multiple sequence alignment