Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   DNH96_RS11015 Genome accession   NZ_CP030715
Coordinates   2284052..2284483 (+) Length   143 a.a.
NCBI ID   WP_096533902.1    Uniprot ID   -
Organism   Staphylococcus pseudintermedius strain VB88     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2275553..2317530 2284052..2284483 within 0


Gene organization within MGE regions


Location: 2275553..2317530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DNH96_RS10935 (DNH96_10980) sufU 2275553..2276002 (+) 450 WP_014614444.1 Fe-S cluster assembly sulfur transfer protein SufU -
  DNH96_RS10940 (DNH96_10985) sufB 2276100..2277497 (+) 1398 WP_014614443.1 Fe-S cluster assembly protein SufB -
  DNH96_RS10945 (DNH96_10990) - 2277565..2278614 (-) 1050 WP_096533923.1 site-specific integrase -
  DNH96_RS10950 (DNH96_10995) - 2278675..2279112 (-) 438 WP_096533921.1 hypothetical protein -
  DNH96_RS10955 - 2279125..2279292 (-) 168 WP_019165842.1 hypothetical protein -
  DNH96_RS10960 (DNH96_11000) - 2279304..2279996 (-) 693 WP_096533919.1 XRE family transcriptional regulator -
  DNH96_RS10965 (DNH96_11005) - 2280191..2280406 (+) 216 WP_065354506.1 transcriptional regulator -
  DNH96_RS10970 (DNH96_11010) - 2280421..2280615 (+) 195 WP_096533917.1 hypothetical protein -
  DNH96_RS10975 (DNH96_11015) - 2280681..2281169 (-) 489 WP_096533915.1 hypothetical protein -
  DNH96_RS10980 (DNH96_11020) - 2281226..2281948 (+) 723 WP_096533913.1 BRO family protein -
  DNH96_RS10985 (DNH96_11025) - 2281945..2282169 (+) 225 WP_015728800.1 hypothetical protein -
  DNH96_RS10990 - 2282157..2282327 (+) 171 WP_181397769.1 hypothetical protein -
  DNH96_RS10995 (DNH96_11030) - 2282416..2282676 (+) 261 WP_096533911.1 DUF1108 family protein -
  DNH96_RS11000 (DNH96_11035) - 2282688..2282906 (+) 219 WP_096533909.1 hypothetical protein -
  DNH96_RS11005 (DNH96_11040) - 2282899..2283384 (+) 486 WP_096533907.1 siphovirus Gp157 family protein -
  DNH96_RS11010 (DNH96_11045) - 2283381..2284052 (+) 672 WP_096533905.1 ERF family protein -
  DNH96_RS11015 (DNH96_11050) ssbA 2284052..2284483 (+) 432 WP_096533902.1 single-stranded DNA-binding protein Machinery gene
  DNH96_RS11020 (DNH96_11060) - 2284667..2285335 (+) 669 WP_096533898.1 putative HNHc nuclease -
  DNH96_RS11025 (DNH96_11065) - 2285351..2285833 (-) 483 WP_096533897.1 hypothetical protein -
  DNH96_RS11030 (DNH96_11070) - 2285877..2286641 (+) 765 WP_096533896.1 conserved phage C-terminal domain-containing protein -
  DNH96_RS11035 (DNH96_11075) - 2286651..2287424 (+) 774 WP_096533895.1 ATP-binding protein -
  DNH96_RS11040 (DNH96_11080) - 2287418..2287597 (+) 180 WP_096533894.1 hypothetical protein -
  DNH96_RS11045 (DNH96_11085) - 2287598..2288032 (+) 435 WP_096533893.1 RusA family crossover junction endodeoxyribonuclease -
  DNH96_RS11050 (DNH96_11090) - 2288016..2288234 (+) 219 WP_096533892.1 hypothetical protein -
  DNH96_RS11055 (DNH96_11095) - 2288247..2288567 (+) 321 WP_096533891.1 hypothetical protein -
  DNH96_RS11060 - 2288564..2288728 (+) 165 WP_179292374.1 hypothetical protein -
  DNH96_RS11065 (DNH96_11100) - 2288728..2289180 (+) 453 WP_096533890.1 DUF3310 domain-containing protein -
  DNH96_RS11070 (DNH96_11105) - 2289177..2290136 (+) 960 WP_096533889.1 DNA cytosine methyltransferase -
  DNH96_RS11075 - 2290207..2290383 (+) 177 WP_019169002.1 hypothetical protein -
  DNH96_RS11080 (DNH96_11110) - 2290383..2290769 (+) 387 WP_238399801.1 hypothetical protein -
  DNH96_RS11085 (DNH96_11120) - 2290985..2291164 (+) 180 WP_096533886.1 hypothetical protein -
  DNH96_RS11090 - 2291165..2291320 (+) 156 WP_179292373.1 hypothetical protein -
  DNH96_RS11095 - 2291321..2291482 (+) 162 WP_179292372.1 hypothetical protein -
  DNH96_RS11100 (DNH96_11125) - 2291506..2291772 (+) 267 WP_037543610.1 hypothetical protein -
  DNH96_RS11105 (DNH96_11130) - 2291774..2292145 (+) 372 WP_096533174.1 hypothetical protein -
  DNH96_RS11110 (DNH96_11135) - 2292164..2292693 (+) 530 Protein_2168 dUTP diphosphatase -
  DNH96_RS11115 (DNH96_11140) - 2292730..2293194 (+) 465 WP_242441896.1 class I SAM-dependent methyltransferase -
  DNH96_RS11120 (DNH96_11145) - 2293191..2293379 (+) 189 WP_189908851.1 DUF1381 domain-containing protein -
  DNH96_RS11125 (DNH96_11150) rinB 2293395..2293565 (+) 171 WP_096533881.1 transcriptional activator RinB -
  DNH96_RS11130 - 2293565..2293717 (+) 153 WP_179292371.1 hypothetical protein -
  DNH96_RS11135 (DNH96_11155) - 2293720..2294139 (+) 420 WP_065354588.1 transcriptional regulator -
  DNH96_RS11140 (DNH96_11160) - 2294338..2294565 (+) 228 WP_096533880.1 hypothetical protein -
  DNH96_RS11145 (DNH96_11165) - 2294635..2295078 (+) 444 WP_096533879.1 terminase small subunit -
  DNH96_RS11150 (DNH96_11170) - 2295065..2296339 (+) 1275 WP_096533878.1 PBSX family phage terminase large subunit -
  DNH96_RS11155 (DNH96_11175) - 2296351..2297796 (+) 1446 WP_096533877.1 phage portal protein -
  DNH96_RS11160 (DNH96_11180) - 2297789..2299141 (+) 1353 WP_096533932.1 minor capsid protein -
  DNH96_RS11165 (DNH96_11185) - 2299138..2299332 (+) 195 WP_096533876.1 LSM domain protein -
  DNH96_RS11170 (DNH96_11190) - 2299484..2300092 (+) 609 WP_096533875.1 DUF4355 domain-containing protein -
  DNH96_RS11175 (DNH96_11195) - 2300110..2301021 (+) 912 WP_096533874.1 capsid protein -
  DNH96_RS11180 (DNH96_11200) - 2301111..2301560 (+) 450 WP_096533873.1 Ig-like domain-containing protein -
  DNH96_RS11185 (DNH96_11205) - 2301572..2301904 (+) 333 WP_096533872.1 phage head-tail connector protein -
  DNH96_RS11190 (DNH96_11210) - 2301901..2302215 (+) 315 WP_060830212.1 hypothetical protein -
  DNH96_RS11195 (DNH96_11215) - 2302205..2302555 (+) 351 WP_096533871.1 HK97-gp10 family putative phage morphogenesis protein -
  DNH96_RS11200 (DNH96_11220) - 2302567..2302962 (+) 396 WP_096533870.1 hypothetical protein -
  DNH96_RS11205 (DNH96_11225) - 2302980..2303633 (+) 654 WP_096533869.1 phage major tail protein, TP901-1 family -
  DNH96_RS11210 (DNH96_11230) - 2303693..2304073 (+) 381 WP_096533868.1 tail assembly chaperone -
  DNH96_RS11215 (DNH96_11235) - 2304103..2304453 (+) 351 WP_096533867.1 hypothetical protein -
  DNH96_RS11220 (DNH96_11240) - 2304470..2307589 (+) 3120 WP_096533866.1 phage tail protein -
  DNH96_RS11225 (DNH96_11245) - 2307602..2308534 (+) 933 WP_096533865.1 phage tail family protein -
  DNH96_RS11230 (DNH96_11250) - 2308547..2309944 (+) 1398 WP_096533864.1 phage tail protein -
  DNH96_RS11235 (DNH96_11255) - 2309948..2310640 (+) 693 WP_096533863.1 hypothetical protein -
  DNH96_RS11240 (DNH96_11260) - 2310652..2311695 (+) 1044 WP_189908852.1 SGNH/GDSL hydrolase family protein -
  DNH96_RS11245 (DNH96_11265) - 2311712..2312983 (+) 1272 WP_096533861.1 phage baseplate upper protein -
  DNH96_RS11250 (DNH96_11270) - 2313016..2313390 (+) 375 WP_096533860.1 hypothetical protein -
  DNH96_RS11255 (DNH96_11275) - 2313514..2315403 (+) 1890 WP_096533859.1 glucosaminidase domain-containing protein -
  DNH96_RS11260 (DNH96_11280) - 2315422..2315676 (+) 255 WP_096533858.1 phage holin -
  DNH96_RS11265 (DNH96_11285) - 2315689..2316444 (+) 756 WP_096533857.1 CHAP domain-containing protein -
  DNH96_RS11270 (DNH96_11290) - 2316509..2316859 (-) 351 WP_096533856.1 hypothetical protein -
  DNH96_RS13380 (DNH96_11295) - 2316856..2317008 (-) 153 WP_096533855.1 ribbon-helix-helix domain-containing protein -
  DNH96_RS11280 (DNH96_11300) - 2317138..2317530 (+) 393 WP_096533854.1 hypothetical protein -

Sequence


Protein


Download         Length: 143 a.a.        Molecular weight: 16281.93 Da        Isoelectric Point: 4.9331

>NTDB_id=301121 DNH96_RS11015 WP_096533902.1 2284052..2284483(+) (ssbA) [Staphylococcus pseudintermedius strain VB88]
MLNRVVLVGRLTKDPEFRTTQSGVEVATFTLAVNRNYKNKNGEQQADFINCIVFRKQAENVNNYLNKGNLAGVDGRLQSR
SYENQEGRRIFVTEVICDNVQFLESKNNNQSNNQQQRGQAPAQDNPFTNANNPIDIEDEDLPF

Nucleotide


Download         Length: 432 bp        

>NTDB_id=301121 DNH96_RS11015 WP_096533902.1 2284052..2284483(+) (ssbA) [Staphylococcus pseudintermedius strain VB88]
ATGCTCAACAGAGTCGTATTGGTAGGTCGATTAACAAAAGACCCGGAATTCAGAACGACGCAATCAGGCGTGGAGGTAGC
AACATTTACATTGGCGGTTAACCGCAATTACAAAAATAAAAACGGAGAACAACAAGCAGACTTTATAAACTGTATTGTTT
TTCGTAAGCAAGCAGAAAATGTGAACAACTATCTAAATAAAGGAAATCTAGCTGGCGTTGATGGTCGCTTACAATCACGC
AGTTACGAAAACCAAGAAGGCCGACGTATATTCGTTACAGAAGTGATTTGTGATAACGTGCAATTTTTAGAGTCTAAAAA
TAACAATCAATCAAACAACCAACAACAAAGAGGTCAAGCGCCTGCACAAGATAATCCATTCACTAACGCAAATAATCCGA
TTGACATCGAAGATGAAGATTTACCCTTTTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

55.233

100

0.664

  ssb Latilactobacillus sakei subsp. sakei 23K

51.176

100

0.608

  ssbB Bacillus subtilis subsp. subtilis str. 168

56.604

74.126

0.42

  ssbA Streptococcus mutans UA159

39.161

100

0.392

  ssbB/cilA Streptococcus mitis NCTC 12261

39.161

100

0.392

  ssbB/cilA Streptococcus pneumoniae TIGR4

39.161

100

0.392

  ssbB Streptococcus sobrinus strain NIDR 6715-7

38.462

100

0.385

  ssbB/cilA Streptococcus pneumoniae Rx1

38.462

100

0.385

  ssbB/cilA Streptococcus pneumoniae D39

38.462

100

0.385

  ssbB/cilA Streptococcus pneumoniae R6

38.462

100

0.385

  ssbB/cilA Streptococcus mitis SK321

38.462

100

0.385

  ssb Glaesserella parasuis strain SC1401

29.944

100

0.371


Multiple sequence alignment