Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SPH_RS10810 Genome accession   NC_010380
Coordinates   2025439..2026455 (-) Length   338 a.a.
NCBI ID   WP_079218398.1    Uniprot ID   -
Organism   Streptococcus pneumoniae Hungary19A-6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2020439..2031455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPH_RS14265 (SPH_2196) - 2020519..2020650 (-) 132 WP_000768904.1 hypothetical protein -
  SPH_RS10765 (SPH_2197) - 2020651..2021841 (-) 1191 WP_000167753.1 acetate kinase -
  SPH_RS10770 (SPH_2198) comYH 2021892..2022845 (-) 954 WP_000345126.1 class I SAM-dependent methyltransferase Machinery gene
  SPH_RS12980 - 2022906..2023500 (-) 595 Protein_2032 methyltransferase domain-containing protein -
  SPH_RS10785 (SPH_2201) comGG/cglG 2023637..2024050 (-) 414 WP_000265625.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPH_RS10790 (SPH_2202) comGF/cglF 2024028..2024489 (-) 462 WP_000250508.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPH_RS10795 (SPH_2203) comGE/cglE 2024452..2024754 (-) 303 WP_191995726.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPH_RS10800 (SPH_2204) comGD/cglD 2024717..2025151 (-) 435 WP_041864711.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPH_RS10805 (SPH_2205) comGC/cglC 2025114..2025437 (-) 324 WP_000738648.1 comG operon protein ComGC Machinery gene
  SPH_RS10810 (SPH_2206) comGB/cglB 2025439..2026455 (-) 1017 WP_079218398.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPH_RS10815 (SPH_2207) comGA/cglA/cilD 2026403..2027344 (-) 942 WP_000249559.1 competence type IV pilus ATPase ComGA Machinery gene
  SPH_RS10820 (SPH_2208) - 2027420..2027785 (-) 366 WP_000286415.1 DUF1033 family protein -
  SPH_RS10825 (SPH_2209) - 2028052..2029307 (+) 1256 WP_088804233.1 ISL3 family transposase -
  SPH_RS10830 (SPH_2210) - 2029356..2030414 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38504.61 Da        Isoelectric Point: 9.6162

>NTDB_id=30047 SPH_RS10810 WP_079218398.1 2025439..2026455(-) (comGB/cglB) [Streptococcus pneumoniae Hungary19A-6]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRAGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSILVLLALTFYKRSSKMRVFSILARLPLLGIFVRTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQSLQSGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=30047 SPH_RS10810 WP_079218398.1 2025439..2026455(-) (comGB/cglB) [Streptococcus pneumoniae Hungary19A-6]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGCGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCCCTAGCCGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTAGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGAATGGTAGGGTTTGTTTCCATACTTGTCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATATTAGCGCGCCTTCCCTTACTTGGAATCTTTGTTCGGACTTATTTGACGGCCTATTACGCGCGTGAATGGGGGA
ATATGATTTCGCAGGGGATGGAGTTGACGCAGATTTTTCAGATGATGCAGGAACAAGGTTCTCAGCTCTTTAAAGAAATC
GGTCAAGACCTAGCTCAATCCCTACAAAGTGGCCGCGAATTTTCTCAAACCATAGCGACCTATCCTTTCTTTAAGAAGGA
GTTGAGTCTCATTATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

95.562

100

0.956
  comGB/cglB Streptococcus pneumoniae Rx1

95.266

100

0.953
  comGB/cglB Streptococcus pneumoniae D39

95.266

100

0.953
  comGB/cglB Streptococcus pneumoniae R6

95.266

100

0.953
  comGB/cglB Streptococcus pneumoniae TIGR4

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.97

100

0.95
  comYB Streptococcus gordonii str. Challis substr. CH1

71.726

99.408

0.713
  comYB Streptococcus mutans UA140

57.595

93.491

0.538
  comYB Streptococcus mutans UA159

57.595

93.491

0.538
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment