Detailed information NTG0030048

Bioinformatically predicted

Overview

• Name: comGA/cglA/cilD
• Type: Machinery gene
• Locus tag: SPH_RS10815
• Genome accession: NC_010380
• Coordinates: 2026403..2027344 (-)
• Length: 313 a.a.
• NCBI ID: WP_000249559.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae Hungary19A-6
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	2028016..2029311	2026403..2027344	flank	672

Gene organization within MGE regions

Location: 2026403..2029311

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPH_RS10815 (SPH_2207)	comGA/cglA/cilD	2026403..2027344 (-)	942	WP_000249559.1	competence type IV pilus ATPase ComGA	Machinery gene
SPH_RS10820 (SPH_2208)	-	2027420..2027785 (-)	366	WP_000286415.1	DUF1033 family protein	-
SPH_RS10825 (SPH_2209)	-	2028052..2029307 (+)	1256	WP_088804233.1	ISL3 family transposase	-

Sequence

Protein

• Length: 313 a.a.
• Molecular weight: 35489.42 Da
• Isoelectric Point: 6.1829

>NTDB_id=30048 SPH_RS10815 WP_000249559.1 2026403..2027344(-) (comGA/cglA/cilD) [Streptococcus pneumoniae Hungary19A-6]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHGLSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide

• Length: 942 bp

>NTDB_id=30048 SPH_RS10815 WP_000249559.1 2026403..2027344(-) (comGA/cglA/cilD) [Streptococcus pneumoniae Hungary19A-6]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGCTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGAAGTGGTAAGACGACCTTGATGCATGGATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGATATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-266)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	98.403	100	0.984
comGA/cglA/cilD	Streptococcus pneumoniae D39	98.403	100	0.984
comGA/cglA/cilD	Streptococcus pneumoniae R6	98.403	100	0.984
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	98.403	100	0.984
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	96.805	100	0.968
comYA	Streptococcus gordonii str. Challis substr. CH1	78.065	99.042	0.773
comYA	Streptococcus mutans UA159	66.238	99.361	0.658
comYA	Streptococcus mutans UA140	66.238	99.361	0.658
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	62.581	99.042	0.62
comGA	Lactococcus lactis subsp. cremoris KW2	55.128	99.681	0.55
comGA	Latilactobacillus sakei subsp. sakei 23K	41.912	86.901	0.364