Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   DOU32_RS09410 Genome accession   NZ_CP030045
Coordinates   1798863..1799138 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain C54     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1745358..1798839 1798863..1799138 flank 24


Gene organization within MGE regions


Location: 1745358..1799138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU32_RS09025 (DOU32_09045) - 1745358..1747571 (-) 2214 WP_002357079.1 RelA/SpoT family protein -
  DOU32_RS09030 (DOU32_09050) - 1747786..1748538 (-) 753 WP_002357078.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DOU32_RS09035 (DOU32_09055) prmA 1748540..1749487 (-) 948 WP_002357075.1 50S ribosomal protein L11 methyltransferase -
  DOU32_RS09040 (DOU32_09060) - 1749503..1749988 (-) 486 WP_002388170.1 DUF3013 family protein -
  DOU32_RS09045 (DOU32_09065) - 1749945..1750622 (-) 678 WP_002364174.1 DNA-3-methyladenine glycosylase -
  DOU32_RS09050 (DOU32_09070) - 1750751..1752028 (+) 1278 WP_002357069.1 replication-associated recombination protein A -
  DOU32_RS09060 (DOU32_09080) - 1752302..1752634 (+) 333 WP_002357068.1 hypothetical protein -
  DOU32_RS09070 (DOU32_09090) - 1752950..1753423 (+) 474 WP_002357065.1 universal stress protein -
  DOU32_RS09075 (DOU32_09095) - 1753535..1754722 (-) 1188 WP_002357064.1 acetate/propionate family kinase -
  DOU32_RS09080 (DOU32_09100) - 1754747..1755754 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  DOU32_RS09085 (DOU32_09105) comGG 1755883..1756236 (-) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  DOU32_RS09090 (DOU32_09110) comGF 1756236..1756670 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  DOU32_RS09095 (DOU32_09115) - 1756660..1756986 (-) 327 WP_002393943.1 type II secretion system protein -
  DOU32_RS09100 (DOU32_09120) - 1757459..1758418 (+) 960 WP_002360751.1 IS30-like element IS1062 family transposase -
  DOU32_RS09110 (DOU32_09130) hemH 1758853..1759794 (-) 942 WP_002357056.1 ferrochelatase -
  DOU32_RS09120 (DOU32_09140) - 1760510..1760782 (-) 273 WP_002370964.1 hypothetical protein -
  DOU32_RS09125 (DOU32_09145) - 1760854..1761054 (-) 201 WP_002357053.1 cold-shock protein -
  DOU32_RS09130 (DOU32_09150) - 1761891..1763137 (-) 1247 Protein_1685 LysM peptidoglycan-binding domain-containing protein -
  DOU32_RS09135 (DOU32_09155) - 1763138..1763371 (-) 234 WP_002384371.1 phage holin -
  DOU32_RS09140 (DOU32_09160) - 1763364..1763585 (-) 222 WP_002364191.1 hypothetical protein -
  DOU32_RS09145 (DOU32_09165) - 1763620..1763775 (-) 156 WP_002364192.1 XkdX family protein -
  DOU32_RS09150 (DOU32_09170) - 1763777..1764097 (-) 321 WP_002389262.1 hypothetical protein -
  DOU32_RS09155 (DOU32_09175) - 1764111..1764602 (-) 492 WP_002364194.1 hypothetical protein -
  DOU32_RS09160 (DOU32_09180) - 1764602..1764889 (-) 288 WP_002357046.1 collagen-like protein -
  DOU32_RS09165 (DOU32_09185) - 1764886..1765482 (-) 597 WP_002357045.1 hypothetical protein -
  DOU32_RS09170 (DOU32_09190) - 1765475..1766356 (-) 882 WP_002357044.1 phage baseplate upper protein -
  DOU32_RS09175 (DOU32_09195) - 1766375..1769182 (-) 2808 WP_002384367.1 phage tail spike protein -
  DOU32_RS09180 (DOU32_09200) - 1769164..1769898 (-) 735 WP_002357042.1 hypothetical protein -
  DOU32_RS09185 (DOU32_09205) - 1769888..1772785 (-) 2898 WP_025187643.1 tape measure protein -
  DOU32_RS09190 (DOU32_09210) gpG 1773033..1773383 (-) 351 WP_002370959.1 phage tail assembly chaperone G -
  DOU32_RS09195 (DOU32_09215) - 1773436..1774284 (-) 849 WP_002384363.1 major tail protein -
  DOU32_RS09200 (DOU32_09220) - 1774285..1774659 (-) 375 WP_002357037.1 phage tail terminator family protein -
  DOU32_RS09205 (DOU32_09225) - 1774662..1775060 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  DOU32_RS09210 (DOU32_09230) - 1775053..1775421 (-) 369 WP_002357034.1 hypothetical protein -
  DOU32_RS09215 (DOU32_09235) - 1775418..1775762 (-) 345 WP_002357033.1 hypothetical protein -
  DOU32_RS09220 (DOU32_09240) - 1775776..1775958 (-) 183 WP_002357032.1 hypothetical protein -
  DOU32_RS09225 (DOU32_09245) - 1775987..1776874 (-) 888 WP_002357030.1 DUF5309 domain-containing protein -
  DOU32_RS09230 (DOU32_09250) - 1776888..1777511 (-) 624 WP_002389133.1 capsid assembly scaffolding protein Gp46 family protein -
  DOU32_RS09235 (DOU32_09255) - 1777729..1778049 (-) 321 WP_002389265.1 hypothetical protein -
  DOU32_RS09240 (DOU32_09260) - 1778114..1778335 (-) 222 WP_002357027.1 hypothetical protein -
  DOU32_RS09245 (DOU32_09265) - 1778332..1780089 (-) 1758 WP_002384360.1 head protein -
  DOU32_RS09250 (DOU32_09270) - 1780064..1781551 (-) 1488 WP_138818577.1 phage portal protein -
  DOU32_RS09255 (DOU32_09275) - 1781563..1782852 (-) 1290 WP_002403123.1 PBSX family phage terminase large subunit -
  DOU32_RS09260 (DOU32_09280) terS 1782824..1783627 (-) 804 WP_002389117.1 phage terminase small subunit -
  DOU32_RS09265 (DOU32_09285) - 1783896..1784813 (+) 918 WP_002370955.1 beta family protein -
  DOU32_RS09270 (DOU32_09290) - 1784851..1785429 (-) 579 WP_002370953.1 sce7726 family protein -
  DOU32_RS09275 (DOU32_09295) - 1785573..1785806 (-) 234 WP_002389079.1 hypothetical protein -
  DOU32_RS09290 (DOU32_09310) - 1786800..1787216 (-) 417 WP_002389289.1 ArpU family phage packaging/lysis transcriptional regulator -
  DOU32_RS09300 (DOU32_09320) - 1788123..1788548 (-) 426 WP_002389212.1 RusA family crossover junction endodeoxyribonuclease -
  DOU32_RS09305 (DOU32_09325) - 1788566..1788865 (-) 300 WP_002389080.1 MazG-like family protein -
  DOU32_RS09310 (DOU32_09330) - 1788866..1789168 (-) 303 WP_002389299.1 hypothetical protein -
  DOU32_RS09315 (DOU32_09335) - 1789172..1790173 (-) 1002 WP_002389256.1 Lin1244/Lin1753 domain-containing protein -
  DOU32_RS09320 (DOU32_09340) - 1790210..1791103 (-) 894 WP_002369907.1 recombinase RecT -
  DOU32_RS09325 (DOU32_09345) - 1791103..1792047 (-) 945 WP_002389314.1 YqaJ viral recombinase family protein -
  DOU32_RS09335 (DOU32_09355) - 1792145..1792372 (-) 228 WP_002364220.1 hypothetical protein -
  DOU32_RS09340 (DOU32_09360) - 1792372..1792695 (-) 324 WP_002369793.1 hypothetical protein -
  DOU32_RS09345 (DOU32_09365) - 1792739..1792924 (-) 186 WP_002364222.1 hypothetical protein -
  DOU32_RS09350 (DOU32_09370) - 1792915..1793109 (-) 195 WP_002364223.1 hypothetical protein -
  DOU32_RS09355 (DOU32_09375) - 1793162..1793344 (+) 183 WP_002364224.1 YegP family protein -
  DOU32_RS09360 (DOU32_09380) - 1793384..1794106 (-) 723 WP_002389018.1 Rha family transcriptional regulator -
  DOU32_RS09365 (DOU32_09385) - 1794145..1794462 (-) 318 WP_002364228.1 hypothetical protein -
  DOU32_RS09370 (DOU32_09390) - 1794468..1794659 (-) 192 WP_002356998.1 hypothetical protein -
  DOU32_RS09380 (DOU32_09400) - 1794950..1795294 (+) 345 WP_002385819.1 helix-turn-helix domain-containing protein -
  DOU32_RS09385 (DOU32_09405) - 1795299..1795946 (+) 648 WP_002409785.1 ImmA/IrrE family metallo-endopeptidase -
  DOU32_RS09390 (DOU32_09410) - 1796064..1796828 (+) 765 WP_002389030.1 LysM peptidoglycan-binding domain-containing protein -
  DOU32_RS09395 (DOU32_09415) - 1796843..1797172 (+) 330 WP_002369911.1 hypothetical protein -
  DOU32_RS09400 (DOU32_09420) - 1797247..1798395 (+) 1149 WP_002389015.1 tyrosine-type recombinase/integrase -
  DOU32_RS09405 (DOU32_09425) comGD 1798423..1798866 (-) 444 WP_002356992.1 competence type IV pilus minor pilin ComGD -
  DOU32_RS09410 (DOU32_09430) comGC/cglC 1798863..1799138 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=298726 DOU32_RS09410 WP_002356991.1 1798863..1799138(-) (comGC/cglC) [Enterococcus faecalis strain C54]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=298726 DOU32_RS09410 WP_002356991.1 1798863..1799138(-) (comGC/cglC) [Enterococcus faecalis strain C54]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396

Multiple sequence alignment