Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DJ564_RS27960 Genome accession   NZ_CP029482
Coordinates   5928836..5930053 (+) Length   405 a.a.
NCBI ID   WP_109634915.1    Uniprot ID   A0A2U8TZ85
Organism   Pseudomonas sp. 31-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5923836..5935053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ564_RS27935 (DJ564_27935) - 5925252..5925551 (+) 300 WP_109634910.1 DUF2845 domain-containing protein -
  DJ564_RS27945 (DJ564_27945) - 5925834..5926187 (-) 354 WP_109634911.1 BON domain-containing protein -
  DJ564_RS27950 (DJ564_27950) pilA 5926500..5926907 (-) 408 WP_010467585.1 pilin Machinery gene
  DJ564_RS27955 (DJ564_27955) pilB 5927133..5928833 (+) 1701 WP_109634913.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DJ564_RS27960 (DJ564_27960) pilC 5928836..5930053 (+) 1218 WP_109634915.1 type II secretion system F family protein Machinery gene
  DJ564_RS27965 (DJ564_27965) pilD 5930055..5930927 (+) 873 WP_109634917.1 A24 family peptidase Machinery gene
  DJ564_RS27970 (DJ564_27970) coaE 5930924..5931547 (+) 624 WP_109634918.1 dephospho-CoA kinase -
  DJ564_RS27975 (DJ564_27975) yacG 5931544..5931744 (+) 201 WP_109634920.1 DNA gyrase inhibitor YacG -
  DJ564_RS27980 (DJ564_27980) - 5931748..5931963 (-) 216 WP_033054332.1 hypothetical protein -
  DJ564_RS27985 (DJ564_27985) - 5932027..5932716 (-) 690 WP_109634921.1 energy-coupling factor ABC transporter permease -
  DJ564_RS27990 (DJ564_27990) - 5933360..5933593 (+) 234 WP_109634923.1 DUF2790 domain-containing protein -
  DJ564_RS27995 (DJ564_27995) - 5933602..5934870 (-) 1269 WP_109634924.1 OprD family porin -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44280.21 Da        Isoelectric Point: 9.9231

>NTDB_id=293094 DJ564_RS27960 WP_109634915.1 5928836..5930053(+) (pilC) [Pseudomonas sp. 31-12]
MAVKAAKISVYAWEGTDRKGSKMTGELSGLNPALIKAQLRKQGINPGRVRKKSASIFSFGKRIKAQEIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFAASLRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFESVFKGFGAELPAFTVMVIGLSEFMQAWWWMMLGVLVAAIFGVR
HAFKTSQGFRDRMDTWLLKLPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALESVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSGIFPSMAIQLTAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=293094 DJ564_RS27960 WP_109634915.1 5928836..5930053(+) (pilC) [Pseudomonas sp. 31-12]
ATGGCGGTCAAAGCAGCAAAAATCAGTGTCTACGCCTGGGAAGGTACGGACCGCAAAGGCTCCAAAATGACCGGCGAGTT
GAGCGGACTCAACCCAGCGTTGATCAAGGCTCAGCTGCGCAAGCAGGGAATCAACCCCGGCAGGGTGCGCAAGAAATCCG
CCTCGATTTTCAGCTTCGGCAAACGTATCAAGGCTCAGGAAATTGCCCTGTTTACCCGGCAGATGGCGACCATGATGAAA
GCCGGCGTGCCACTGCTGCAGTCGTTCGACATCATCGGCGAAGGCTTCGACAACCCGGCCATGCGCAAACTGGTGGACGA
GGTGAAACAGGAAGTCGCGGCGGGTAACAGCTTCGCCGCGTCCCTACGCAAAAAGCCGCAGTATTTCGATGAGCTGTACT
GCAACCTCGTCGATGCCGGTGAACAGGCCGGCGCGCTGGACACCCTGCTGGAACGGGTAGCGACCTACAAGGAAAAGAGC
GAAAGCCTGAAAGCGAAGATCAAGAAAGCCATGACCTACCCGCTGGCCGTGGTCTTCGTGGCGATCATTGTCACCGGGAT
TCTGCTGGTCAAGGTGGTGCCGCAGTTCGAGTCGGTGTTCAAGGGCTTTGGCGCGGAGTTGCCGGCCTTCACCGTAATGG
TCATAGGCCTGTCGGAGTTCATGCAGGCGTGGTGGTGGATGATGCTGGGCGTGTTGGTCGCCGCCATCTTCGGTGTGCGC
CACGCCTTTAAAACCTCCCAGGGCTTTCGGGACCGGATGGACACCTGGCTACTGAAGCTGCCGCTTATCGGCACCTTGAT
GTACAAGTCTGCCGTGGCCCGTTACGCCCGCACCCTGTCGACCACGTTCGCCGCTGGCGTGCCGTTGGTCGAAGCCCTGG
AGTCAGTGGCTGGCGCGACCGGCAACATCGTATTCAAACGTGCCGTGCTGCGCATCAAGCAGGACGTGTCGACCGGCATG
CAGCTGAATTTTTCCATGCGCACCTCGGGCATCTTTCCCAGCATGGCGATCCAGTTGACCGCCATTGGCGAAGAATCCGG
TGCACTGGACGACATGCTCGATAAGGTCGCGAGCTTCTACGAGGATGAAGTGGACAATATGGTCGATAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGTGTTATTGTCGGTGGCCTGGTGGTTGCGATGTACCTGCCCATTTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8TZ85

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

59.069

100

0.595
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilG Neisseria gonorrhoeae MS11

42.432

99.506

0.422
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilG Neisseria meningitidis 44/76-A

41.935

99.506

0.417
  pilC Vibrio campbellii strain DS40M4

40.148

100

0.402
  pilC Thermus thermophilus HB27

37.656

99.012

0.373

Multiple sequence alignment