Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DJ564_RS27955 Genome accession   NZ_CP029482
Coordinates   5927133..5928833 (+) Length   566 a.a.
NCBI ID   WP_109634913.1    Uniprot ID   A0A2U8TXV2
Organism   Pseudomonas sp. 31-12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5922133..5933833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ564_RS27930 (DJ564_27930) - 5922310..5925252 (+) 2943 WP_109634908.1 DUF748 domain-containing protein -
  DJ564_RS27935 (DJ564_27935) - 5925252..5925551 (+) 300 WP_109634910.1 DUF2845 domain-containing protein -
  DJ564_RS27945 (DJ564_27945) - 5925834..5926187 (-) 354 WP_109634911.1 BON domain-containing protein -
  DJ564_RS27950 (DJ564_27950) pilA 5926500..5926907 (-) 408 WP_010467585.1 pilin Machinery gene
  DJ564_RS27955 (DJ564_27955) pilB 5927133..5928833 (+) 1701 WP_109634913.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DJ564_RS27960 (DJ564_27960) pilC 5928836..5930053 (+) 1218 WP_109634915.1 type II secretion system F family protein Machinery gene
  DJ564_RS27965 (DJ564_27965) pilD 5930055..5930927 (+) 873 WP_109634917.1 A24 family peptidase Machinery gene
  DJ564_RS27970 (DJ564_27970) coaE 5930924..5931547 (+) 624 WP_109634918.1 dephospho-CoA kinase -
  DJ564_RS27975 (DJ564_27975) yacG 5931544..5931744 (+) 201 WP_109634920.1 DNA gyrase inhibitor YacG -
  DJ564_RS27980 (DJ564_27980) - 5931748..5931963 (-) 216 WP_033054332.1 hypothetical protein -
  DJ564_RS27985 (DJ564_27985) - 5932027..5932716 (-) 690 WP_109634921.1 energy-coupling factor ABC transporter permease -
  DJ564_RS27990 (DJ564_27990) - 5933360..5933593 (+) 234 WP_109634923.1 DUF2790 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62827.99 Da        Isoelectric Point: 6.2103

>NTDB_id=293093 DJ564_RS27955 WP_109634913.1 5927133..5928833(+) (pilB) [Pseudomonas sp. 31-12]
MNDIALSGLAKQLVLAELLTDQSAQQAYQQAQRNKISLVSYLVQNRLVKSRQVAEIASEHFGMPLLDLNCLDKETQPRGL
VSEKLVRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLNTEAILVEDDKLGDAIDKFFDSQSTGLEDMADVDLDG
LDIESVDDNKKDSIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPSQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKALYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLHNMGIKGFNIATSVTLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGLFTIYEPVGCDQCNRGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLSKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=293093 DJ564_RS27955 WP_109634913.1 5927133..5928833(+) (pilB) [Pseudomonas sp. 31-12]
ATGAATGACATCGCCCTTAGCGGTCTGGCCAAGCAATTGGTTCTGGCCGAACTGCTCACTGACCAAAGCGCGCAACAGGC
GTATCAGCAAGCTCAACGCAACAAAATCTCCCTGGTCAGTTATCTGGTGCAGAACAGACTGGTGAAGAGCCGACAGGTCG
CCGAGATTGCCTCGGAGCATTTCGGCATGCCGCTGCTCGACCTCAATTGCCTGGATAAAGAAACCCAACCCCGAGGGCTG
GTCAGCGAGAAACTGGTCCGTCAGCACCACGCCCTGCCCCTCTGGCGACGCGGCAACAAGCTGTTCGTGGGGGTTTCCGA
TCCGACCAATCATCAAGCCATCAATGACATCCAGTTCAGCACAGGACTGAACACCGAAGCCATTCTGGTGGAGGACGATA
AACTCGGCGATGCCATCGATAAATTCTTCGACAGCCAATCCACCGGCCTGGAAGACATGGCCGATGTTGACCTTGACGGG
CTGGACATCGAGTCGGTCGATGACAATAAAAAGGACTCCATCGCAGGCCAGGATGCCGATGACGCGCCGGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGATGCGATCAAGAGCGGTTCGTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGGG
TGCGGATGCGCACCGACGGCATGCTGCGCGAAGTCGCCAAGCCGCCGAGCCAACTGGCCAACCGCATTGCGGCGCGGCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGACGGATCAAGATGCGCCTGTCCAAGACCAA
ATCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CGCAAATGGGCATCGACGCCCTCGGCTACGAGCCTGAGCAGAAAGCCTTGTACATGGCTGCGCTGAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGGCCGACCGGTTCGGGCAAGACCGTGTCGCTCTACACCGGGCTGAACATCCTCAACACCGTGGA
CATCAACATCTCTACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAAG
GGCTGGATTTCGCCCAGGCGCTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGTGAAATCCGCGACCTC
GAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGCTTGCACAACATGGGCATTAAGGGCTTCAACATTGCCACCTCGGTGACCCTGATCATCGCCCAGCGGC
TGGCGCGCAAACTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGCGAGGCGTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTTGTTCACGATCTATGAGCCGGTCGGCTGCGATCAGTGCAACCGCGGTTACAAAGGGCGAGTGGGGAT
TTATGAAGTGGTGAAGAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAAATCGACATCCAGA
TGCGCAAGGACGGTTTCAACGACTTGCGCACTTCGGGATTGAGCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8TXV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.97

99.47

0.587
  pilB Acinetobacter baumannii D1279779

58.511

99.647

0.583
  pilB Legionella pneumophila strain ERS1305867

56.106

99.823

0.56
  pilB Vibrio cholerae strain A1552

51.071

98.94

0.505
  pilF Neisseria gonorrhoeae MS11

49.911

99.47

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.73

98.057

0.488
  pilB Vibrio campbellii strain DS40M4

48.936

99.647

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.752

93.463

0.362

Multiple sequence alignment