Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VIBHAR_RS00135 Genome accession   NC_009783
Coordinates   25817..27565 (-) Length   582 a.a.
NCBI ID   WP_041853271.1    Uniprot ID   -
Organism   Vibrio campbellii ATCC BAA-1116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 20817..32565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIBHAR_RS00115 (VIBHAR_00025) - 21522..22361 (-) 840 WP_005532700.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  VIBHAR_RS00120 (VIBHAR_00026) - 22431..23924 (-) 1494 WP_012126429.1 SPOR domain-containing protein -
  VIBHAR_RS00125 (VIBHAR_00027) aroB 23978..25078 (-) 1101 WP_012126430.1 3-dehydroquinate synthase -
  VIBHAR_RS00130 (VIBHAR_00028) aroK 25110..25628 (-) 519 WP_010443904.1 shikimate kinase AroK -
  VIBHAR_RS00135 (VIBHAR_00029) pilQ 25817..27565 (-) 1749 WP_041853271.1 type IV pilus secretin PilQ Machinery gene
  VIBHAR_RS00140 (VIBHAR_00030) pilP 27600..28115 (-) 516 WP_012126432.1 pilus assembly protein PilP Machinery gene
  VIBHAR_RS00145 (VIBHAR_00031) pilO 28105..28698 (-) 594 WP_012126433.1 type 4a pilus biogenesis protein PilO Machinery gene
  VIBHAR_RS00150 (VIBHAR_00032) pilN 28691..29266 (-) 576 WP_012126434.1 PilN domain-containing protein Machinery gene
  VIBHAR_RS00155 (VIBHAR_00033) pilM 29250..30266 (-) 1017 WP_041853270.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63814.76 Da        Isoelectric Point: 4.5130

>NTDB_id=29007 VIBHAR_RS00135 WP_041853271.1 25817..27565(-) (pilQ) [Vibrio campbellii ATCC BAA-1116]
MKMRHGLTLTPRKLKGLFSLWFFLLLAPFVAAEEVQSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVQDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGDGEGDDGGSSVGIDDFLNVNLAATSPNA
TSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=29007 VIBHAR_RS00135 WP_041853271.1 25817..27565(-) (pilQ) [Vibrio campbellii ATCC BAA-1116]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAAAAGGGCTCTTCTCTTTATGGTTCTTTCTACTTTTAGC
CCCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCAGCGATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGCAAGATTTCTCGACAGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTGGCTTATATAGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTATAACCAATTTAACTTGGTCGTCTC
TGACTCGGTTGAGGGTAACTTGACCTTGCGTTTGGATGGTGTGCCTTGGCAGCAAGTACTCGACATTATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAATGTCATTCTCGTTGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGGGAGGGCAACATCAGTATGCTTTCTGATCGAGGCAGCATGACCATTGATGAGCGAACAA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCGCGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACTTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCCTGTACGATGGCGGCGGTGATG
GTGAAGGTGATGACGGTGGCAGCAGTGTGGGTATTGACGACTTTCTCAACGTTAACCTTGCTGCGACCAGTCCAAATGCG
ACCAGTATTGCCTTCCAAGTTGCGAAGTTAGGTTCGGATACCTTACTAGATTTGGAACTTTCGGCGCTGCAACAAGAATC
GAAAGCAGAAATTATTTCCAGTCCACGTTTGATCACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCGAGATCC
CTTACCTTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACGCCACAG
ATTACGCCTGATAACCGTTTGGTGTTGGATTTGAGCGTTACGCAAGACAGACCGGGCCAAGTGGTAAAAACAGGCACTGG
TGAGGCGGTGGCGATCGATACGCAACGTATTGGTACACAAGTTTTAGTTAATAATGGCGAGACAGTCGTGTTGGGCGGTA
TTTTTCAACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTTTTCCGT
CGAAGTTACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

98.797

100

0.988
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.289

97.595

0.754
  pilQ Vibrio cholerae strain A1552

77.289

97.595

0.754
  pilQ Legionella pneumophila strain ERS1305867

41.742

94.674

0.395
  pilQ Legionella pneumophila strain Lp02

41.742

94.674

0.395
  pilQ Pseudomonas aeruginosa PAK

40.142

96.735

0.388
  pilQ Acinetobacter baumannii D1279779

37.271

100

0.385
  pilQ Acinetobacter baumannii strain A118

37.271

100

0.385
  comQ Acinetobacter baylyi ADP1

37.005

99.828

0.369

Multiple sequence alignment