Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VIBHAR_RS00155 Genome accession   NC_009783
Coordinates   29250..30266 (-) Length   338 a.a.
NCBI ID   WP_041853270.1    Uniprot ID   -
Organism   Vibrio campbellii ATCC BAA-1116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 24250..35266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIBHAR_RS00130 (VIBHAR_00028) aroK 25110..25628 (-) 519 WP_010443904.1 shikimate kinase AroK -
  VIBHAR_RS00135 (VIBHAR_00029) pilQ 25817..27565 (-) 1749 WP_041853271.1 type IV pilus secretin PilQ Machinery gene
  VIBHAR_RS00140 (VIBHAR_00030) pilP 27600..28115 (-) 516 WP_012126432.1 pilus assembly protein PilP Machinery gene
  VIBHAR_RS00145 (VIBHAR_00031) pilO 28105..28698 (-) 594 WP_012126433.1 type 4a pilus biogenesis protein PilO Machinery gene
  VIBHAR_RS00150 (VIBHAR_00032) pilN 28691..29266 (-) 576 WP_012126434.1 PilN domain-containing protein Machinery gene
  VIBHAR_RS00155 (VIBHAR_00033) pilM 29250..30266 (-) 1017 WP_041853270.1 type IV pilus biogenesis protein PilM Machinery gene
  VIBHAR_RS00160 (VIBHAR_00034) - 30445..33012 (+) 2568 WP_012126436.1 penicillin-binding protein 1A -
  VIBHAR_RS00165 (VIBHAR_00035) oxyR 33091..33999 (-) 909 WP_012126437.1 DNA-binding transcriptional regulator OxyR -
  VIBHAR_RS00170 (VIBHAR_00036) - 34174..34902 (+) 729 WP_012126438.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37883.33 Da        Isoelectric Point: 6.4195

>NTDB_id=29011 VIBHAR_RS00155 WP_041853270.1 29250..30266(-) (pilM) [Vibrio campbellii ATCC BAA-1116]
MDKLLVTGIDIGHHSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=29011 VIBHAR_RS00155 WP_041853270.1 29250..30266(-) (pilM) [Vibrio campbellii ATCC BAA-1116]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATCACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGGGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAATCTCCATTCCCTGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTTGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATGTTCACCGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTACTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGACGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAAGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTGTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

97.929

100

0.979
  pilM Vibrio cholerae strain A1552

49.708

100

0.503

Multiple sequence alignment