Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C5B40_RS08650 Genome accession   NZ_CP028917
Coordinates   1804408..1805628 (-) Length   406 a.a.
NCBI ID   WP_016253888.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain JB2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1799408..1810628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5B40_RS08605 - 1799589..1800038 (-) 450 WP_003094670.1 MOSC domain-containing protein -
  C5B40_RS08610 - 1800035..1800664 (-) 630 WP_023098063.1 DUF1780 domain-containing protein -
  C5B40_RS08615 - 1800797..1801222 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  C5B40_RS08620 - 1801219..1801689 (+) 471 WP_003094664.1 hypothetical protein -
  C5B40_RS08625 - 1801686..1802375 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  C5B40_RS08630 - 1802481..1802690 (+) 210 WP_003094660.1 hypothetical protein -
  C5B40_RS08635 yacG 1802727..1802927 (-) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  C5B40_RS08640 coaE 1802924..1803535 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  C5B40_RS08645 pilD 1803532..1804404 (-) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  C5B40_RS08650 pilC 1804408..1805628 (-) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  C5B40_RS08655 pilB 1805632..1807332 (-) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C5B40_RS08660 pilA 1807561..1808013 (+) 453 WP_024947914.1 pilin Machinery gene
  C5B40_RS08670 nadC 1808201..1809049 (-) 849 WP_024947913.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44549.37 Da        Isoelectric Point: 9.6983

>NTDB_id=288518 C5B40_RS08650 WP_016253888.1 1804408..1805628(-) (pilC) [Pseudomonas aeruginosa strain JB2]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFEGFGAELPAFTQMIVNLSEFMQEWWFFIILAIAIFGFAFK
ELHKRSQKFRDTLDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=288518 C5B40_RS08650 WP_016253888.1 1804408..1805628(-) (pilC) [Pseudomonas aeruginosa strain JB2]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAAGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTGAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAGGGCTTCGACAACCCCAACATGCGCAAGCTTGTGGATGA
AATCAAACAGGAAGTTTCCTCAGGTAACAGCCTAGCCAACTCCTTGAGAAAAAAGCCCCAGTATTTTGACGAGCTTTATT
GCAACCTGGTAGATGCAGGGGAACAGTCTGGCGCCTTGGAAAACCTTCTCGATCGGGTGGCAACCTATAAAGAAAAGACG
GAATCACTGAAAGCCAAGATCAAAAAGGCGATGACCTATCCCATTGCCGTCATCATTGTCGCACTGATTGTATCTGCGAT
CCTCCTGATTAAAGTGGTTCCACAATTTCAGTCGGTCTTTGAAGGTTTCGGCGCGGAACTTCCCGCCTTTACCCAGATGA
TTGTCAATCTATCGGAGTTCATGCAGGAGTGGTGGTTCTTCATCATACTGGCGATAGCGATATTTGGCTTTGCATTCAAA
GAATTGCATAAACGCTCACAAAAATTCCGTGACACACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCTGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGTTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCAATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGCCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.543

99.754

0.764
  pilC Acinetobacter baumannii D1279779

61.386

99.507

0.611
  pilC Acinetobacter baylyi ADP1

60.837

100

0.608
  pilC Legionella pneumophila strain ERS1305867

55.051

97.537

0.537
  pilG Neisseria gonorrhoeae MS11

46.173

99.754

0.461
  pilG Neisseria meningitidis 44/76-A

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

42.065

97.783

0.411
  pilC Thermus thermophilus HB27

36.908

98.768

0.365

Multiple sequence alignment