Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C5B40_RS08660 Genome accession   NZ_CP028917
Coordinates   1807561..1808013 (+) Length   150 a.a.
NCBI ID   WP_024947914.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain JB2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1802561..1813013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5B40_RS08635 yacG 1802727..1802927 (-) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  C5B40_RS08640 coaE 1802924..1803535 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  C5B40_RS08645 pilD 1803532..1804404 (-) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  C5B40_RS08650 pilC 1804408..1805628 (-) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  C5B40_RS08655 pilB 1805632..1807332 (-) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C5B40_RS08660 pilA 1807561..1808013 (+) 453 WP_024947914.1 pilin Machinery gene
  C5B40_RS08670 nadC 1808201..1809049 (-) 849 WP_024947913.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C5B40_RS08675 - 1809335..1811590 (+) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  C5B40_RS08680 ampD 1811741..1812307 (+) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15622.02 Da        Isoelectric Point: 8.4827

>NTDB_id=288520 C5B40_RS08660 WP_024947914.1 1807561..1808013(+) (pilA) [Pseudomonas aeruginosa strain JB2]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG
VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide


Download         Length: 453 bp        

>NTDB_id=288520 C5B40_RS08660 WP_024947914.1 1807561..1808013(+) (pilA) [Pseudomonas aeruginosa strain JB2]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT
TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG
AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG
GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC
TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA
AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

100

100

1
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.682

100

0.48
  pilA Acinetobacter baumannii strain A118

45.333

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.575

97.333

0.453
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.675

100

0.447
  pilA Vibrio cholerae C6706

42.675

100

0.447
  pilA Vibrio cholerae strain A1552

42.675

100

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

48.507

89.333

0.433
  comP Acinetobacter baylyi ADP1

40.789

100

0.413
  pilA2 Legionella pneumophila strain ERS1305867

39.865

98.667

0.393
  pilA/pilA1 Eikenella corrodens VA1

39.456

98

0.387
  pilA2 Legionella pneumophila str. Paris

39.189

98.667

0.387

Multiple sequence alignment