Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   CDG55_RS02535 Genome accession   NZ_CP028561
Coordinates   277018..277434 (+) Length   138 a.a.
NCBI ID   WP_416333005.1    Uniprot ID   -
Organism   Acinetobacter sp. WCHA45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 272018..282434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG55_RS02510 (CDG55_02510) - 272522..273943 (+) 1422 WP_087535974.1 FAD-dependent oxidoreductase -
  CDG55_RS02515 (CDG55_02515) - 274015..274188 (+) 174 Protein_249 metal-dependent hydrolase -
  CDG55_RS02520 (CDG55_02520) - 274369..274959 (+) 591 WP_087535976.1 LemA family protein -
  CDG55_RS02525 (CDG55_02525) - 275045..276058 (+) 1014 WP_416333004.1 TPM domain-containing protein -
  CDG55_RS02530 (CDG55_02530) - 276052..276612 (+) 561 WP_087535978.1 TPM domain-containing protein -
  CDG55_RS02535 (CDG55_02535) pilA2 277018..277434 (+) 417 WP_416333005.1 pilin Machinery gene
  CDG55_RS02540 (CDG55_02540) - 277504..279471 (+) 1968 WP_087535980.1 hypothetical protein -
  CDG55_RS02545 (CDG55_02545) - 279461..280315 (+) 855 WP_087535981.1 glycosyltransferase family 2 protein -
  CDG55_RS02550 (CDG55_02550) - 280324..282045 (+) 1722 WP_087535982.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14296.37 Da        Isoelectric Point: 9.5191

>NTDB_id=285504 CDG55_RS02535 WP_416333005.1 277018..277434(+) (pilA2) [Acinetobacter sp. WCHA45]
MQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRAQVTEGVNLMAGAKASVTEVFNDSGTFPATNSAAGLPTATSIKGSY
VTKVELATGGVIVATYGNKANSSISGGTCRVTPANNGGSISWTGTCSFDQKWRPKAFR

Nucleotide


Download         Length: 417 bp        

>NTDB_id=285504 CDG55_RS02535 WP_416333005.1 277018..277434(+) (pilA2) [Acinetobacter sp. WCHA45]
ATGCAAAAGGGTTTTACTCTTATCGAATTGATGATCGTTGTAGCGATCATTGGTATTTTGGCGGCAATTGCGATCCCTGC
TTACCAAGACTACACCATTCGTGCCCAAGTAACTGAAGGGGTAAACCTAATGGCTGGAGCAAAAGCATCTGTAACAGAGG
TTTTTAATGATTCAGGTACTTTTCCAGCAACGAATAGTGCTGCTGGTCTACCAACAGCGACTTCAATTAAAGGTTCTTAT
GTAACTAAGGTTGAGTTGGCAACAGGTGGTGTAATTGTTGCCACTTATGGCAACAAGGCAAATTCTAGTATTAGTGGTGG
AACTTGTCGTGTTACCCCAGCTAATAACGGTGGTAGTATTTCTTGGACAGGTACTTGCTCATTTGATCAAAAATGGCGTC
CAAAAGCGTTCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-24)

Pilin (bacterial filament) (Pilin)

(34-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

49.645

100

0.507
  pilA2 Legionella pneumophila strain ERS1305867

49.645

100

0.507
  pilA Ralstonia pseudosolanacearum GMI1000

40.506

100

0.464
  comP Acinetobacter baylyi ADP1

42.177

100

0.449
  pilE Neisseria gonorrhoeae strain FA1090

49.18

88.406

0.435
  pilE Neisseria gonorrhoeae MS11

47.967

89.13

0.428
  pilA Acinetobacter baumannii strain A118

39.259

97.826

0.384
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.519

97.826

0.377
  pilA/pilA1 Eikenella corrodens VA1

53.608

70.29

0.377
  pilA Haemophilus influenzae Rd KW20

39.37

92.029

0.362
  pilA Vibrio parahaemolyticus RIMD 2210633

43.478

83.333

0.362

Multiple sequence alignment