Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CDG55_RS02480 Genome accession   NZ_CP028561
Coordinates   262794..264938 (+) Length   714 a.a.
NCBI ID   WP_162620847.1    Uniprot ID   -
Organism   Acinetobacter sp. WCHA45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 257794..269938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG55_RS02460 (CDG55_02460) comM 259782..260840 (+) 1059 WP_087535965.1 pilus assembly protein PilM Machinery gene
  CDG55_RS02465 (CDG55_02465) pilN 260840..261463 (+) 624 WP_087535966.1 PilN domain-containing protein Machinery gene
  CDG55_RS02470 (CDG55_02470) comO 261460..262206 (+) 747 WP_087535967.1 type 4a pilus biogenesis protein PilO Machinery gene
  CDG55_RS02475 (CDG55_02475) pilP 262206..262733 (+) 528 WP_087535968.1 pilus assembly protein PilP Machinery gene
  CDG55_RS02480 (CDG55_02480) pilQ 262794..264938 (+) 2145 WP_162620847.1 type IV pilus secretin PilQ family protein Machinery gene
  CDG55_RS02485 (CDG55_02485) aroK 264949..265515 (+) 567 WP_087535970.1 shikimate kinase AroK -
  CDG55_RS02490 (CDG55_02490) aroB 265540..266622 (+) 1083 WP_087535971.1 3-dehydroquinate synthase -
  CDG55_RS02495 (CDG55_02495) - 266642..267508 (+) 867 WP_087535972.1 hypothetical protein -

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77729.42 Da        Isoelectric Point: 8.8235

>NTDB_id=285503 CDG55_RS02480 WP_162620847.1 262794..264938(+) (pilQ) [Acinetobacter sp. WCHA45]
MNHAFRQFSMGAVAIAIMQAASAQVSMTNIVPMSLADQGTEIRVMFNGLPPQPQAYQLEKPSRLILDFDKTQQNLKQASI
PVSTNEASTIDVAADDQRSRLVVNLKEAGAFTTRVEGNTFILKINAVQPVNAVRNATVVRQPQQGVSNIGFQRGKDGEGL
VVIDLLGNNTPVDVQQQGSKIVVRTMGNKIPAHLARRLNTNDFATPVASVDAYNDKGNGVITIQSAGSYEYMAYQAENKL
TISLKRPEDKSSPKAKAQVYTGNKISLDFQDIEVRRVLQLLADFTSINMVAADTVQGNITLRLKDVPWDQVLDIVLKTKN
LDKRRNGNVIWIAPVSELIKAEEEEAKAIAQSIKLSPIQTEYIKLSYAKAADIEKLITQGKNSGSGNSSSGPNNNADPLG
DSVGSLLSPRGTISTDPRTNTLIINDTSQKIDQIRKMVDLLDVSVKQVMIEARIVSASTDFTKEMGVKWGILSQGITSNN
DLLVGGSNTTLWNLRKPTKDSDTGGWKYEIERPSNLNVDLGATAVGASRIAFGLISLSDFMLDLELSAVQADGYGEVIST
PKVMTADKQTAKIESGTQVPFSSSTGTGANATPKIEFKDVVLSLDVTPSITPDGKVQMNLDIKKDSIAGVTPQGQFYFNK
NMVNTNVLVNNGETVVLGGIFEQENLNKQTKVPFLGDLPFMGKLFRRDSKTENKRELLIFVTPRIVNDTLTRNH

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=285503 CDG55_RS02480 WP_162620847.1 262794..264938(+) (pilQ) [Acinetobacter sp. WCHA45]
ATGAATCATGCATTTCGTCAGTTTTCTATGGGTGCTGTTGCCATCGCAATCATGCAAGCTGCATCTGCACAAGTGAGTAT
GACGAATATTGTTCCAATGAGTTTGGCTGATCAAGGTACAGAAATTCGTGTGATGTTTAATGGATTGCCTCCACAACCGC
AGGCATATCAATTGGAAAAACCTTCTCGATTGATTTTAGATTTTGATAAAACGCAACAAAATTTGAAACAGGCATCAATA
CCAGTATCAACTAATGAAGCATCAACAATTGATGTTGCAGCTGATGATCAGCGTTCACGTCTAGTTGTTAATTTAAAGGA
AGCAGGAGCATTTACGACACGTGTTGAAGGGAACACGTTTATTCTTAAAATAAATGCAGTTCAACCAGTAAATGCTGTTC
GTAATGCTACGGTTGTCAGACAACCACAGCAAGGTGTATCAAATATTGGTTTCCAGCGCGGTAAGGATGGCGAGGGGTTG
GTTGTTATTGACCTATTGGGAAATAACACACCTGTCGATGTACAGCAGCAAGGTAGTAAGATTGTGGTGCGAACAATGGG
GAACAAAATTCCAGCCCATTTGGCGCGTCGTTTAAATACCAACGATTTTGCTACACCTGTCGCAAGTGTAGATGCTTATA
ATGATAAAGGTAACGGTGTTATCACCATTCAGTCTGCTGGTAGTTATGAATATATGGCTTACCAAGCGGAAAATAAATTA
ACGATTAGTCTTAAGCGCCCAGAAGATAAATCTTCTCCGAAAGCTAAAGCCCAAGTCTATACTGGGAATAAAATTTCATT
AGATTTTCAAGATATTGAGGTGCGTCGAGTTCTGCAGCTGTTGGCTGATTTTACCAGTATAAACATGGTTGCAGCTGATA
CCGTACAAGGTAATATTACGCTGCGGTTGAAAGATGTGCCTTGGGATCAGGTTCTGGATATTGTATTAAAAACCAAGAAT
TTGGATAAGCGCCGTAATGGCAATGTAATCTGGATTGCACCTGTTTCTGAGTTAATTAAGGCAGAAGAAGAAGAAGCCAA
AGCAATTGCACAAAGTATTAAGCTTTCTCCAATACAAACAGAATATATTAAGTTGAGTTATGCAAAAGCTGCTGATATTG
AGAAATTAATTACTCAGGGTAAAAATAGTGGCTCAGGTAACAGCTCTTCAGGTCCGAATAATAATGCTGATCCATTGGGA
GATAGTGTTGGGAGTTTGTTAAGTCCTCGTGGTACGATTTCTACTGACCCTCGCACGAATACACTGATTATTAATGACAC
GTCACAAAAAATTGATCAAATTCGTAAAATGGTCGATTTGCTGGATGTATCAGTTAAGCAGGTAATGATTGAGGCACGTA
TTGTCAGTGCAAGTACCGATTTTACGAAAGAAATGGGTGTTAAATGGGGTATTCTATCGCAAGGTATTACTAGTAATAAT
GATTTGTTAGTAGGTGGTAGTAATACTACTTTATGGAACTTAAGAAAGCCTACTAAAGATAGTGATACAGGGGGGTGGAA
ATATGAAATTGAACGTCCAAGTAACCTAAATGTTGATTTGGGGGCAACAGCAGTAGGTGCGAGTCGTATTGCTTTTGGTT
TAATCAGTTTGTCAGATTTTATGCTGGATCTAGAACTGTCTGCGGTTCAGGCGGATGGTTATGGTGAAGTCATTTCAACG
CCTAAAGTTATGACTGCGGATAAACAAACTGCAAAAATTGAGTCAGGTACTCAAGTTCCTTTTTCATCCTCAACGGGTAC
TGGGGCAAATGCAACGCCGAAAATCGAATTTAAAGATGTGGTATTAAGTTTAGATGTAACGCCGAGTATTACACCAGATG
GAAAGGTTCAAATGAATCTTGATATTAAGAAAGATAGTATTGCTGGTGTAACCCCTCAAGGACAATTCTATTTTAATAAA
AACATGGTGAATACTAATGTCTTGGTTAATAATGGTGAAACGGTGGTATTAGGCGGTATTTTTGAACAAGAAAATCTAAA
CAAACAAACCAAGGTGCCATTTTTAGGTGATTTACCTTTCATGGGCAAATTATTCCGTCGTGATTCGAAAACTGAAAATA
AACGTGAACTACTTATTTTTGTGACACCAAGAATTGTTAATGACACTTTGACGAGAAATCATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(292-334)

AMIN domain (AMIN)

(37-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(361-447)

Bacterial type II and III secretion system protein (Secretin)

(548-706)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

75.691

100

0.768
  pilQ Acinetobacter baumannii strain A118

75.552

100

0.766
  comQ Acinetobacter baylyi ADP1

73.427

100

0.735
  pilQ Pseudomonas aeruginosa PAK

39.484

100

0.408
  pilQ Legionella pneumophila strain Lp02

36.82

100

0.37
  pilQ Legionella pneumophila strain ERS1305867

36.85

99.58

0.367

Multiple sequence alignment