Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE/blpR   Type   Regulator
Locus tag   SM12261_RS03610 Genome accession   NZ_CP028414
Coordinates   669501..670241 (+) Length   246 a.a.
NCBI ID   WP_001019941.1    Uniprot ID   A0AAX2L835
Organism   Streptococcus mitis NCTC 12261     
Function   activate transcription of early competence genes; regulation of comX expression (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 638459..693605 669501..670241 within 0


Gene organization within MGE regions


Location: 638459..693605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS03460 (SM12261_0669) aroC 638712..639878 (+) 1167 WP_001269850.1 chorismate synthase -
  SM12261_RS03465 (SM12261_0670) - 639888..640991 (+) 1104 WP_001140859.1 prephenate dehydrogenase -
  SM12261_RS03470 (SM12261_0671) - 641002..641340 (+) 339 WP_000065969.1 YlbF/YmcA family competence regulator -
  SM12261_RS03475 (SM12261_0672) aroA 641432..642715 (+) 1284 WP_004238699.1 3-phosphoshikimate 1-carboxyvinyltransferase -
  SM12261_RS03480 (SM12261_0673) - 642708..643184 (+) 477 WP_001151349.1 shikimate kinase -
  SM12261_RS03485 (SM12261_0674) pheA 643181..644029 (+) 849 WP_000686445.1 prephenate dehydratase -
  SM12261_RS03490 (SM12261_0675) - 644026..645336 (+) 1311 WP_000032338.1 LCP family protein -
  SM12261_RS03495 (SM12261_0676) - 645457..646302 (+) 846 WP_001061995.1 LicD family protein -
  SM12261_RS03500 (SM12261_0677) - 646292..647395 (+) 1104 WP_000225887.1 glycosyltransferase -
  SM12261_RS03505 (SM12261_0678) - 647397..648383 (+) 987 WP_000446965.1 glycosyltransferase family 2 protein -
  SM12261_RS03510 (SM12261_0679) - 648399..649868 (+) 1470 WP_004238693.1 O-antigen polysaccharide polymerase Wzy family protein -
  SM12261_RS03515 (SM12261_0680) - 649861..650895 (+) 1035 WP_000461491.1 hypothetical protein -
  SM12261_RS03520 (SM12261_0681) mecA 651102..651839 (+) 738 WP_000782682.1 adaptor protein MecA Regulator
  SM12261_RS03525 (SM12261_0682) - 651991..653277 (+) 1287 WP_000216346.1 homoserine dehydrogenase -
  SM12261_RS03530 (SM12261_0683) thrB 653279..654148 (+) 870 WP_000692425.1 homoserine kinase -
  SM12261_RS03535 (SM12261_0684) msrB 654361..655299 (+) 939 WP_000818182.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SM12261_RS03540 (SM12261_0685) - 655360..657084 (+) 1725 WP_000681728.1 ABC transporter ATP-binding protein -
  SM12261_RS03545 (SM12261_0686) - 657086..658834 (+) 1749 WP_001254284.1 ABC transporter ATP-binding protein -
  SM12261_RS03550 (SM12261_0687) - 659092..660351 (+) 1260 WP_000870440.1 TRZ/ATZ family protein -
  SM12261_RS03560 (SM12261_0688) rplJ 660602..661102 (+) 501 WP_001287267.1 50S ribosomal protein L10 -
  SM12261_RS03565 (SM12261_0689) rplL 661175..661543 (+) 369 WP_001196965.1 50S ribosomal protein L7/L12 -
  SM12261_RS03570 (SM12261_0691) pezA 662071..662547 (+) 477 WP_000579609.1 type II toxin-antitoxin system antitoxin PezA -
  SM12261_RS03575 (SM12261_0692) pezT 662547..663317 (+) 771 WP_000405376.1 type II toxin-antitoxin system toxin PezT -
  SM12261_RS03585 (SM12261_0695) - 664974..665405 (+) 432 WP_025169729.1 hypothetical protein -
  SM12261_RS03590 (SM12261_0696) - 665408..665797 (+) 390 Protein_615 replication initiation factor domain-containing protein -
  SM12261_RS03595 (SM12261_0697) - 665960..666430 (+) 471 WP_000447038.1 MarR family transcriptional regulator -
  SM12261_RS03600 (SM12261_0698) - 666430..668706 (+) 2277 WP_000401876.1 ATP-binding cassette domain-containing protein -
  SM12261_RS03605 (SM12261_0699) - 669158..669490 (+) 333 WP_000656278.1 LytTR family DNA-binding domain-containing protein -
  SM12261_RS03610 (SM12261_0700) comE/blpR 669501..670241 (+) 741 WP_001019941.1 response regulator transcription factor Regulator
  SM12261_RS03615 (SM12261_0701) - 670470..671615 (+) 1146 WP_000209246.1 low temperature requirement protein A -
  SM12261_RS03620 - 671912..671980 (+) 69 WP_373852502.1 Maff2 family mobile element protein -
  SM12261_RS03625 (SM12261_0702) - 672305..672988 (+) 684 WP_000492701.1 LexA family transcriptional regulator -
  SM12261_RS03630 (SM12261_0703) - 672991..674406 (+) 1416 WP_000219659.1 Y-family DNA polymerase -
  SM12261_RS03635 - 674403..674768 (+) 366 WP_000981959.1 hypothetical protein -
  SM12261_RS03640 - 674758..675048 (+) 291 WP_001080187.1 DUF5960 family protein -
  SM12261_RS03645 (SM12261_0704) - 675343..675702 (+) 360 WP_000529547.1 SAG1252 family conjugative relaxosome accessory protein -
  SM12261_RS03650 (SM12261_0705) mobC 675712..676077 (+) 366 WP_000263039.1 plasmid mobilization relaxosome protein MobC -
  SM12261_RS03655 (SM12261_0706) - 676064..677926 (+) 1863 WP_000261797.1 SAG1250 family conjugative relaxase -
  SM12261_RS03660 (SM12261_0707) - 677970..678818 (-) 849 WP_000837479.1 helix-turn-helix domain-containing protein -
  SM12261_RS09675 - 679171..679386 (+) 216 WP_265094651.1 ATP-binding cassette domain-containing protein -
  SM12261_RS03670 (SM12261_0708) - 679379..679813 (+) 435 WP_135781820.1 ATP-binding cassette domain-containing protein -
  SM12261_RS09480 (SM12261_0709) - 679826..679996 (+) 171 WP_000711945.1 hypothetical protein -
  SM12261_RS03680 (SM12261_0710) gdhA 680277..681623 (-) 1347 WP_000199543.1 NADP-specific glutamate dehydrogenase -
  SM12261_RS03685 (SM12261_0711) - 681864..682799 (+) 936 WP_000255154.1 dihydroorotate oxidase -
  SM12261_RS03690 (SM12261_0712) holA 682833..683870 (+) 1038 WP_135781821.1 DNA polymerase III subunit delta -
  SM12261_RS03700 (SM12261_0714) sodA 684241..684846 (+) 606 WP_000974733.1 superoxide dismutase SodA -
  SM12261_RS03705 (SM12261_0715) - 685008..685538 (+) 531 WP_001224347.1 YutD family protein -
  SM12261_RS03710 (SM12261_0716) rlmN 685559..686644 (+) 1086 WP_000804766.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SM12261_RS03715 (SM12261_0717) - 686644..687126 (+) 483 WP_000976729.1 VanZ family protein -
  SM12261_RS03720 (SM12261_0718) - 687128..688669 (+) 1542 WP_000025380.1 ABC-F family ATP-binding cassette domain-containing protein -
  SM12261_RS03725 (SM12261_0719) - 688723..689526 (-) 804 WP_000717398.1 peptidylprolyl isomerase -
  SM12261_RS03730 (SM12261_0720) - 689831..690055 (-) 225 WP_001041099.1 helix-turn-helix transcriptional regulator -
  SM12261_RS03735 (SM12261_0721) - 690058..690636 (-) 579 WP_000742118.1 hypothetical protein -
  SM12261_RS09485 (SM12261_0722) - 690897..691040 (-) 144 WP_001067872.1 hypothetical protein -
  SM12261_RS03740 (SM12261_0723) rpsP 691216..691488 (+) 273 WP_000268760.1 30S ribosomal protein S16 -
  SM12261_RS03745 (SM12261_0724) kphA 691508..691747 (+) 240 WP_000379617.1 RNA-binding protein KphA -
  SM12261_RS03750 (SM12261_0725) - 691861..692412 (+) 552 WP_004239319.1 hypothetical protein -
  SM12261_RS03755 (SM12261_0726) - 692520..693308 (+) 789 WP_000821028.1 alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 28746.12 Da        Isoelectric Point: 5.9117

>NTDB_id=284635 SM12261_RS03610 WP_001019941.1 669501..670241(+) (comE/blpR) [Streptococcus mitis NCTC 12261]
MNIFILEDNFLQQTRIENVVKKILVDNKIEYRHFEVYGKPQQLLEDISERGNHQLFLLDIEIKDDDKRGLDVAREIRKLD
SQAVIAFVTSHSEFMPVSFEYLVSAIDFIDKELPEPLFIKRIENVILSVSDNQGITVSEDSFIFTGSKAQIQVPFKDLLY
IETSTIAHKLTLYSKRDIVEFYGQLSDILKQEPRLFQCHRSFIVNPYNISLIDKENRLVHFQNGSSCIVSRLKIRPLMKV
IERLHD

Nucleotide


Download         Length: 741 bp        

>NTDB_id=284635 SM12261_RS03610 WP_001019941.1 669501..670241(+) (comE/blpR) [Streptococcus mitis NCTC 12261]
GTGAATATTTTTATACTAGAAGATAATTTTTTACAACAGACTAGAATTGAAAATGTTGTCAAAAAAATTTTGGTGGATAA
TAAGATTGAGTATAGGCATTTTGAGGTTTATGGAAAACCTCAACAGCTTTTAGAGGACATTTCAGAGAGAGGAAATCATC
AATTATTTTTACTTGATATTGAAATAAAAGATGATGACAAAAGAGGATTAGACGTTGCTAGAGAAATTAGAAAACTTGAT
TCTCAAGCAGTGATTGCTTTTGTAACATCTCATTCTGAGTTTATGCCTGTATCTTTTGAATATTTAGTATCAGCTATTGA
TTTCATTGATAAAGAATTACCTGAACCATTATTTATCAAACGAATTGAAAATGTTATTTTAAGTGTTAGCGATAATCAAG
GAATAACAGTATCTGAGGACTCTTTTATATTTACAGGATCCAAAGCTCAAATTCAAGTCCCTTTTAAAGATTTATTGTAT
ATTGAAACATCAACAATTGCTCATAAGTTGACTCTCTATTCAAAACGAGACATAGTTGAATTTTATGGACAATTGTCAGA
TATTCTTAAACAGGAACCACGTTTATTTCAATGTCATAGATCATTTATCGTAAATCCTTATAATATATCTTTAATTGATA
AAGAAAATCGGTTAGTTCATTTTCAGAACGGGTCCTCTTGTATTGTCTCTAGATTAAAAATTCGTCCACTTATGAAAGTT
ATCGAAAGACTTCATGACTAA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(3-122)

LytTr DNA-binding domain (LytTR)

(151-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE/blpR Streptococcus mutans UA159

52.066

98.374

0.512
  comE/comE1 Streptococcus equinus JB1

44.538

96.748

0.431
  comE Streptococcus mitis SK321

36.8

100

0.374
  comE Streptococcus mitis NCTC 12261

36.8

100

0.374
  comE Streptococcus pneumoniae Rx1

36.4

100

0.37
  comE Streptococcus pneumoniae R6

36.4

100

0.37
  comE Streptococcus pneumoniae D39

36.4

100

0.37
  comE Streptococcus pneumoniae TIGR4

36.4

100

0.37
  comE Streptococcus infantis strain Atu-4

36

100

0.366
  comE/comE2 Streptococcus equinus JB1

36.885

99.187

0.366
  comE/comE1 Streptococcus gordonii str. Challis substr. CH1

36.437

100

0.366
  comE/comE2 Streptococcus gordonii strain NCTC7865

36.437

100

0.366

Multiple sequence alignment