Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4J99_RS02070 Genome accession   NZ_CP027758
Coordinates   469188..470513 (+) Length   441 a.a.
NCBI ID   WP_124402423.1    Uniprot ID   -
Organism   Pseudomonas synxantha strain R2-54-08W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 464188..475513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J99_RS02050 (C4J99_0417) - 464251..466713 (-) 2463 WP_420902458.1 penicillin-binding protein 1A -
  C4J99_RS02055 (C4J99_0419) pilM 466946..467602 (+) 657 WP_124399052.1 type IV pilus biogenesis protein PilM -
  C4J99_RS02060 (C4J99_0420) - 467599..468150 (+) 552 WP_124399053.1 PilN domain-containing protein -
  C4J99_RS02065 (C4J99_0421) - 468147..469118 (+) 972 WP_124399054.1 pilus assembly protein PilP -
  C4J99_RS02070 (C4J99_0422) pilQ 469188..470513 (+) 1326 WP_124402423.1 type IV pilus secretin PilQ Machinery gene
  C4J99_RS02075 (C4J99_0423) aroK 470518..471036 (+) 519 WP_003187914.1 shikimate kinase AroK -
  C4J99_RS02080 (C4J99_0424) aroB 471162..472262 (+) 1101 WP_024072965.1 3-dehydroquinate synthase -
  C4J99_RS02085 (C4J99_0425) - 472273..473844 (+) 1572 WP_124399055.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47936.96 Da        Isoelectric Point: 5.0282

>NTDB_id=280212 C4J99_RS02070 WP_124402423.1 469188..470513(+) (pilQ) [Pseudomonas synxantha strain R2-54-08W]
MAAAVPNRPGLIHLPPPDRALPVSYTGEKLSLNFQDIDLRAVLQQIADVAGLNLVTSDEVQGAITLRLKDVPWDQALDLV
LQTKGLDKRVKAGVLLVAPADELAARELLVLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSMTGFEGATDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLSRGNWGAGGISKPLADGAEPPA
NPPSSPFVDLGSLTGTSGLGIAYITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMAVKVTKDEPDYLNRLNDVPPIKKNEVNAKVLVRNGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=280212 C4J99_RS02070 WP_124402423.1 469188..470513(+) (pilQ) [Pseudomonas synxantha strain R2-54-08W]
ATGGCTGCCGCTGTGCCCAATCGCCCGGGCCTGATCCACCTGCCGCCGCCTGACCGTGCCTTGCCGGTCAGCTATACGGG
CGAAAAACTGAGCCTTAATTTCCAGGACATCGACCTGCGTGCGGTATTGCAGCAAATCGCCGATGTCGCCGGGCTCAACC
TGGTGACCAGCGACGAGGTGCAGGGCGCGATCACCCTGCGGCTCAAGGACGTGCCCTGGGACCAGGCGCTGGACCTGGTG
CTGCAAACCAAGGGCCTGGACAAACGGGTGAAGGCCGGCGTGCTGCTGGTGGCGCCCGCCGATGAATTGGCCGCTCGCGA
GCTGCTGGTGCTGGAGTCGCGCAAGCAGATGGCCGAGCTGGCACCGTTGCGTCGAGAATTATTGCAGGTCAATTACGCCA
AGGCTGCGGACCTGGCCAAGCTGTTCCAGTCGATGACCGGCTTTGAAGGTGCCACGGATGAGCGCGGCTCGGTGGCGGTG
GATGACCGCACCAACAACATCATCGCCTACCAGACCGGCGAGCGCCTCGAAGAGCTGCGGCGCATCGTGGCGCAACTGGA
TATCCCGGTACGCCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAATGTCGACTACGACAAAAGCCTGGGCGTGCGCT
GGGGTGGGCGGCTCAGCCGCGGCAACTGGGGCGCGGGGGGTATTTCCAAACCCCTGGCGGACGGCGCCGAGCCACCGGCA
AACCCGCCCAGTTCGCCCTTTGTCGACTTGGGTTCGCTAACCGGCACGTCGGGGCTGGGTATCGCCTACATCACCGACAA
CCTGTTGCTGGATCTCGAACTGACGGCGATGGAGAAAAGCGGCAACGGCGAAATCGTTTCGCAACCCAAGGTGGTCACGT
CCGATAAGGAGACCGCACGCATCCTCAAAGGCACCGAGATTCCCTATCAGGAGTCCACCTCCCAGGGCGCCACCTCGGTG
TCGTTCAAGGAAGCGTCCCTTTCACTGGAGGTCACGCCGCAAATCACCCCCGATGACCACGTGATCATGGCGGTCAAGGT
CACCAAGGACGAGCCCGACTACCTGAACCGGCTCAATGACGTGCCGCCGATCAAGAAAAACGAAGTCAACGCCAAGGTTC
TGGTCCGGAATGGCGAGACCATCGTCATTGGTGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(54-99)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.396

99.093

0.707
  comQ Acinetobacter baylyi ADP1

44.346

100

0.454
  pilQ Acinetobacter baumannii D1279779

41.29

100

0.435
  pilQ Acinetobacter baumannii strain A118

41.29

100

0.435
  pilQ Vibrio campbellii strain DS40M4

44.111

98.186

0.433
  pilQ Legionella pneumophila strain Lp02

45.476

95.238

0.433
  pilQ Legionella pneumophila strain ERS1305867

45

95.238

0.429
  pilQ Vibrio cholerae strain A1552

43.488

97.506

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.488

97.506

0.424
  pilQ Neisseria meningitidis M1080

36.952

98.186

0.363
  pilQ Neisseria meningitidis MC58

37.063

97.279

0.361

Multiple sequence alignment