Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4J96_RS01960 Genome accession   NZ_CP027725
Coordinates   458032..459357 (+) Length   441 a.a.
NCBI ID   WP_124427924.1    Uniprot ID   -
Organism   Pseudomonas orientalis strain R2-66-08W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453032..464357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J96_RS01940 (C4J96_0402) - 453108..455570 (-) 2463 WP_420893424.1 penicillin-binding protein 1A -
  C4J96_RS01945 (C4J96_0404) pilM 455790..456446 (+) 657 WP_124427923.1 type IV pilus assembly protein PilM -
  C4J96_RS01950 (C4J96_0405) - 456443..456994 (+) 552 WP_124356686.1 PilN domain-containing protein -
  C4J96_RS01955 (C4J96_0406) - 456991..457962 (+) 972 WP_124425843.1 pilus assembly protein PilP -
  C4J96_RS01960 (C4J96_0407) pilQ 458032..459357 (+) 1326 WP_124427924.1 type IV pilus secretin PilQ Machinery gene
  C4J96_RS01965 (C4J96_0408) aroK 459362..459880 (+) 519 WP_057724420.1 shikimate kinase AroK -
  C4J96_RS01970 (C4J96_0409) aroB 460006..461106 (+) 1101 WP_124425844.1 3-dehydroquinate synthase -
  C4J96_RS01975 (C4J96_0410) - 461117..462691 (+) 1575 WP_124425845.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47737.53 Da        Isoelectric Point: 5.1069

>NTDB_id=279424 C4J96_RS01960 WP_124427924.1 458032..459357(+) (pilQ) [Pseudomonas orientalis strain R2-66-08W]
MAAAVPNRVNLIHLPPPESASSAGYTGDTLNLNFQNIDVRAALQQIADVAGLNLVTGDDVQGSITLRLKDVPWDQALDLV
LQAKGLDKRVKAGVLLVAPADELAARELLTLEARKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGFEGATNERGSVAV
DDRTNNIIAYQTAERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGNWGAGGISRPVADGAQPPQ
NPPSAPFVDLGSLTGTSGLGLAFITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMVVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVRAEKKSELLVFLTPRIMNNQAIAASR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=279424 C4J96_RS01960 WP_124427924.1 458032..459357(+) (pilQ) [Pseudomonas orientalis strain R2-66-08W]
ATGGCTGCTGCTGTACCCAATCGCGTCAACCTTATCCATCTGCCGCCGCCCGAAAGCGCATCGTCGGCCGGCTATACGGG
CGACACACTGAACCTCAATTTCCAGAACATCGATGTGCGCGCGGCATTGCAGCAAATCGCCGATGTCGCCGGCCTGAACC
TGGTGACCGGTGACGACGTGCAGGGTTCTATCACTCTGCGGCTCAAGGACGTGCCTTGGGACCAGGCGCTGGACCTGGTA
TTGCAGGCCAAGGGGTTGGATAAACGGGTGAAGGCCGGCGTCCTGCTGGTGGCCCCCGCCGATGAGTTGGCCGCACGCGA
ACTGCTGACGCTGGAGGCGCGCAAGCAGATGGCTGAGCTGGCGCCGTTACGCCGGGAGTTACTGCAGGTCAATTATGCCA
AGGCGGCGGACCTGGCCAAGCTGTTCCAGTCCGTGACCGGCTTTGAGGGCGCGACCAACGAGCGCGGCTCGGTGGCGGTG
GACGACCGCACCAACAATATCATCGCCTACCAGACCGCGGAACGGCTGGACGAACTGCGGCGGATCGTGGCGCAGTTGGA
CATCCCGGTGCGCCAGGTGATGATCGAGGCGCGCATTGTCGAGGCCAATGTCGATTACGACAAAAGCCTGGGGGTGCGCT
GGGGCGGGCGGCTCAATCGCGGCAATTGGGGCGCCGGCGGTATTTCCAGGCCCGTGGCGGATGGCGCGCAGCCACCCCAG
AATCCGCCCAGTGCACCCTTTGTCGACCTGGGTTCGCTGACCGGCACCTCCGGCCTGGGGCTGGCCTTTATCACCGACAA
CCTGCTGCTGGACCTTGAGCTGACCGCCATGGAAAAGTCCGGCAACGGCGAAATCGTCTCGCAGCCCAAGGTGGTCACCT
CGGACAAGGAAACCGCGCGCATTCTCAAGGGCACCGAGATTCCCTACCAGGAATCCACGTCCCAGGGCGCCACTTCGGTG
TCGTTCAAGGAAGCCTCGTTGTCATTGGAGGTTACGCCGCAGATCACCCCCGACGATCATGTGATCATGGTGGTCAAGGT
CACCAAGGACGAGCCCGACTACCTGAACAAGCTCAACGACGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTCC
TGGTCAAGGATGGCGAGACCATTGTCATCGGCGGGGTTTTTTCCAATACCCAGAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCGGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATCATGAATAACCAGGCGATTGCTGCGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(276-432)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(55-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.872

98.866

0.701
  comQ Acinetobacter baylyi ADP1

42.763

100

0.442
  pilQ Acinetobacter baumannii strain A118

41.164

100

0.433
  pilQ Acinetobacter baumannii D1279779

41.164

100

0.433
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.357

97.279

0.422
  pilQ Vibrio cholerae strain A1552

43.357

97.279

0.422
  pilQ Vibrio campbellii strain DS40M4

43.735

95.918

0.419
  pilQ Legionella pneumophila strain Lp02

43.128

95.692

0.413
  pilQ Legionella pneumophila strain ERS1305867

43.095

95.238

0.41
  pilQ Neisseria gonorrhoeae strain FA1090

35.217

100

0.367
  pilQ Neisseria meningitidis MC58

35.076

100

0.365
  pilQ Neisseria meningitidis M1080

35.076

100

0.365

Multiple sequence alignment