Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4J97_RS02050 Genome accession   NZ_CP027724
Coordinates   479115..480440 (+) Length   441 a.a.
NCBI ID   WP_124425567.1    Uniprot ID   -
Organism   Pseudomonas orientalis strain L1-3-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 474115..485440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J97_RS02030 (C4J97_0411) - 474189..476651 (-) 2463 WP_420893540.1 penicillin-binding protein 1A -
  C4J97_RS02035 (C4J97_0413) pilM 476872..477528 (+) 657 WP_124422067.1 type IV pilus assembly protein PilM -
  C4J97_RS02040 (C4J97_0414) - 477525..478076 (+) 552 WP_124422068.1 PilN domain-containing protein -
  C4J97_RS02045 (C4J97_0415) - 478073..479044 (+) 972 WP_124422069.1 pilus assembly protein PilP -
  C4J97_RS02050 (C4J97_0416) pilQ 479115..480440 (+) 1326 WP_124425567.1 type IV pilus secretin PilQ Machinery gene
  C4J97_RS02055 (C4J97_0417) aroK 480445..480963 (+) 519 WP_057724420.1 shikimate kinase AroK -
  C4J97_RS02060 (C4J97_0418) aroB 481089..482189 (+) 1101 WP_124422070.1 3-dehydroquinate synthase -
  C4J97_RS02065 (C4J97_0419) - 482200..483774 (+) 1575 WP_124422071.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47753.63 Da        Isoelectric Point: 5.1135

>NTDB_id=279400 C4J97_RS02050 WP_124425567.1 479115..480440(+) (pilQ) [Pseudomonas orientalis strain L1-3-08]
MAAAVPNRVDLIHLPPPESASSAGYTGDRMNLNFQNIDLRAALQQIADVAGLNLVAGDDVQGSITLRLKDVPWDQALDLV
LQAKGLDKRVKAGVLLVAPADELAARELLTLEARKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGFEGATDERGSVAV
DDRTNNIIAYQTAERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGNWGAGGISRPVTDGAQPPL
NPPSAPFVDLGSLTGTSGLGLAFITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMVVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKGGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVRAEKKSELLVFLTPRIMNNQAIAASR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=279400 C4J97_RS02050 WP_124425567.1 479115..480440(+) (pilQ) [Pseudomonas orientalis strain L1-3-08]
ATGGCTGCTGCTGTACCCAATCGCGTCGACCTTATCCATCTGCCGCCGCCCGAAAGCGCATCGTCGGCCGGCTATACGGG
CGACAGAATGAACCTCAATTTCCAGAACATCGATTTGCGCGCGGCATTGCAGCAAATTGCCGATGTAGCCGGCCTGAACC
TGGTGGCCGGTGACGACGTACAGGGGTCGATCACCCTGCGGCTCAAGGATGTGCCTTGGGACCAGGCGCTGGACCTGGTA
TTGCAGGCCAAGGGGTTGGATAAGCGGGTGAAGGCCGGCGTCCTGCTGGTGGCTCCCGCCGATGAACTGGCCGCACGCGA
ATTGCTGACGCTGGAGGCGCGCAAGCAGATGGCTGAGCTGGCACCGTTACGCCGGGAGTTGCTGCAGGTCAATTATGCCA
AGGCAGCGGACCTGGCCAAGCTGTTCCAATCCGTGACCGGCTTTGAGGGTGCAACCGACGAGCGCGGCTCGGTGGCGGTG
GACGATCGCACCAACAATATCATCGCCTACCAGACCGCAGAGCGGCTGGACGAACTGCGGCGGATCGTGGCGCAGTTGGA
CATCCCGGTGCGTCAGGTGATGATCGAGGCGCGCATTGTCGAGGCCAATGTCGATTACGACAAAAGCCTGGGGGTGCGCT
GGGGCGGGCGGCTCAATCGCGGCAATTGGGGCGCCGGCGGTATTTCCAGGCCCGTGACGGATGGCGCGCAGCCACCCCTG
AACCCACCCAGTGCACCCTTTGTCGACCTGGGTTCGCTGACCGGCACCTCCGGCCTGGGGCTGGCCTTTATCACCGACAA
CCTGCTGCTGGACCTTGAGCTGACCGCCATGGAGAAGTCCGGCAACGGCGAAATCGTCTCACAGCCCAAGGTGGTCACCT
CGGACAAGGAAACCGCGCGCATCCTCAAGGGCACCGAGATTCCCTATCAGGAATCCACGTCCCAGGGCGCCACTTCGGTG
TCGTTCAAGGAAGCTTCGCTGTCGCTGGAGGTCACGCCGCAGATCACCCCCGACGATCATGTGATCATGGTGGTCAAGGT
CACCAAGGATGAGCCCGACTACCTGAACAAACTCAACGACGTGCCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCC
TGGTCAAGGGTGGCGAGACCATTGTCATCGGCGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCGGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATCATGAATAACCAGGCGATTGCTGCGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(276-432)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(53-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.615

100

0.696
  comQ Acinetobacter baylyi ADP1

42.325

100

0.438
  pilQ Acinetobacter baumannii strain A118

41.379

100

0.435
  pilQ Acinetobacter baumannii D1279779

41.379

100

0.435
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.89

97.279

0.417
  pilQ Vibrio cholerae strain A1552

42.89

97.279

0.417
  pilQ Legionella pneumophila strain ERS1305867

43.78

94.785

0.415
  pilQ Vibrio campbellii strain DS40M4

43.262

95.918

0.415
  pilQ Legionella pneumophila strain Lp02

43.571

95.238

0.415
  pilQ Neisseria meningitidis M1080

35.294

100

0.367
  pilQ Neisseria gonorrhoeae strain FA1090

35.526

100

0.367
  pilQ Neisseria meningitidis MC58

35.385

100

0.365

Multiple sequence alignment