Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C3V42_RS05950 Genome accession   NZ_CP027235
Coordinates   1229698..1230138 (+) Length   146 a.a.
NCBI ID   WP_106013961.1    Uniprot ID   A0A502LKJ3
Organism   Haemophilus sp. oral taxon 036 strain F0629     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1224698..1235138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3V42_RS05920 (C3V42_05925) tadA 1224907..1225440 (-) 534 WP_048952801.1 tRNA adenosine(34) deaminase TadA -
  C3V42_RS05925 (C3V42_05930) - 1225440..1226291 (-) 852 WP_106013957.1 thymidylate synthase -
  C3V42_RS05930 (C3V42_05935) lgt 1226301..1227107 (-) 807 WP_106013958.1 prolipoprotein diacylglyceryl transferase -
  C3V42_RS05935 (C3V42_05940) - 1227116..1227913 (-) 798 WP_106013959.1 sulfite exporter TauE/SafE family protein -
  C3V42_RS05940 (C3V42_05945) rppH 1227913..1228503 (-) 591 WP_005631202.1 RNA pyrophosphohydrolase -
  C3V42_RS05945 (C3V42_05950) ampD 1229029..1229583 (-) 555 WP_106013960.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C3V42_RS05950 (C3V42_05955) pilA 1229698..1230138 (+) 441 WP_106013961.1 prepilin peptidase-dependent pilin Machinery gene
  C3V42_RS05955 (C3V42_05960) pilB 1230135..1231529 (+) 1395 WP_106013962.1 GspE/PulE family protein Machinery gene
  C3V42_RS05960 (C3V42_05965) pilC 1231526..1232743 (+) 1218 WP_106013963.1 type II secretion system F family protein Machinery gene
  C3V42_RS05965 (C3V42_05970) pilD 1232743..1233432 (+) 690 WP_048952810.1 A24 family peptidase Machinery gene
  C3V42_RS05970 (C3V42_05975) era 1233667..1234575 (-) 909 WP_009766812.1 GTPase Era -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15036.26 Da        Isoelectric Point: 8.4607

>NTDB_id=275948 C3V42_RS05950 WP_106013961.1 1229698..1230138(+) (pilA) [Haemophilus sp. oral taxon 036 strain F0629]
MKLTTQTTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKSDVELCVYSTGKPSSCSGGSNGIA
ADITTAKGYVKSVTTSNGAITVAGNGTLDGMSYTLTAEGDSAKGVTWKTTCGTTNADIFPAGFCSK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=275948 C3V42_RS05950 WP_106013961.1 1229698..1230138(+) (pilA) [Haemophilus sp. oral taxon 036 strain F0629]
ATGAAACTAACTACACAAACTACCTTGAAGAAAGGGTTTACCTTAATTGAATTGATGATAGTCATCGCCATCATCGCGAT
TTTAGCCACTATCGCTATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCTTCTGCAC
CTTACAAGTCAGATGTGGAATTATGCGTTTATAGCACAGGCAAACCTTCTAGTTGCTCAGGAGGAAGCAATGGAATTGCA
GCTGATATTACAACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAGCGGGTAATGGTAC
ATTAGATGGAATGAGTTACACACTCACAGCGGAAGGCGATAGTGCAAAAGGCGTTACATGGAAAACCACCTGTGGCACAA
CGAATGCCGATATTTTCCCTGCTGGATTCTGTTCAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A502LKJ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

81.25

98.63

0.801
  pilA Haemophilus influenzae Rd KW20

77.778

98.63

0.767
  pilA Glaesserella parasuis strain SC1401

55.333

100

0.568
  pilA2 Legionella pneumophila str. Paris

41.497

100

0.418
  pilA2 Legionella pneumophila strain ERS1305867

41.497

100

0.418
  comP Acinetobacter baylyi ADP1

41.379

99.315

0.411
  pilA Vibrio campbellii strain DS40M4

36.986

100

0.37
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.063

97.945

0.363

Multiple sequence alignment