Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C3V42_RS05955 Genome accession   NZ_CP027235
Coordinates   1230135..1231529 (+) Length   464 a.a.
NCBI ID   WP_106013962.1    Uniprot ID   A0A502LH77
Organism   Haemophilus sp. oral taxon 036 strain F0629     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1225135..1236529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3V42_RS05925 (C3V42_05930) - 1225440..1226291 (-) 852 WP_106013957.1 thymidylate synthase -
  C3V42_RS05930 (C3V42_05935) lgt 1226301..1227107 (-) 807 WP_106013958.1 prolipoprotein diacylglyceryl transferase -
  C3V42_RS05935 (C3V42_05940) - 1227116..1227913 (-) 798 WP_106013959.1 sulfite exporter TauE/SafE family protein -
  C3V42_RS05940 (C3V42_05945) rppH 1227913..1228503 (-) 591 WP_005631202.1 RNA pyrophosphohydrolase -
  C3V42_RS05945 (C3V42_05950) ampD 1229029..1229583 (-) 555 WP_106013960.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C3V42_RS05950 (C3V42_05955) pilA 1229698..1230138 (+) 441 WP_106013961.1 prepilin peptidase-dependent pilin Machinery gene
  C3V42_RS05955 (C3V42_05960) pilB 1230135..1231529 (+) 1395 WP_106013962.1 GspE/PulE family protein Machinery gene
  C3V42_RS05960 (C3V42_05965) pilC 1231526..1232743 (+) 1218 WP_106013963.1 type II secretion system F family protein Machinery gene
  C3V42_RS05965 (C3V42_05970) pilD 1232743..1233432 (+) 690 WP_048952810.1 A24 family peptidase Machinery gene
  C3V42_RS05970 (C3V42_05975) era 1233667..1234575 (-) 909 WP_009766812.1 GTPase Era -
  C3V42_RS05975 (C3V42_05980) rnc 1234572..1235255 (-) 684 WP_106013964.1 ribonuclease III -
  C3V42_RS05980 (C3V42_05985) lepB 1235257..1236306 (-) 1050 WP_106013965.1 signal peptidase I -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52518.84 Da        Isoelectric Point: 6.0968

>NTDB_id=275949 C3V42_RS05955 WP_106013962.1 1230135..1231529(+) (pilB) [Haemophilus sp. oral taxon 036 strain F0629]
MIDLTSAKPRVKAQNGEIFTISPDLWGRNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKLVEPVLL
ESSQLKELLQQLAPNQPKVEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQNNASDIHLESLSDHFQVRFRIDGVL
QAQPPLSKNLANRIISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKVVLRAQQNKPVELSFSELG
MTETQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYQGRIGVYQFLHWQQNGYQTDFKNLHTSGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=275949 C3V42_RS05955 WP_106013962.1 1230135..1231529(+) (pilB) [Haemophilus sp. oral taxon 036 strain F0629]
ATGATCGACTTAACTAGTGCAAAACCTCGTGTAAAAGCACAAAATGGCGAGATATTTACGATCTCGCCAGACCTATGGGG
GCGTAATCAGCAACAACAGTCGCTACTCTTACGTTATTTTGCGTTACCACTCAAAGAAGAAAATAATCGTCTCTGGCTAG
GTGTAGATTCTCTTTCTAATCTTTCCGCCTGTGAAACTATTGCGTTTATAACCGGTAAACTTGTTGAGCCAGTTTTATTA
GAAAGCTCTCAACTTAAAGAACTATTACAGCAACTTGCTCCTAATCAACCTAAAGTGGAAGAGCAAGTTAAATATTACCA
ACATCAAGAACAGTCCCATCTTGAACAACAAGATGATGAGCCTGTCATTCGCTTGCTTAATCAAATTTTTGAATCCGCTT
TACAAAACAATGCCTCAGATATTCATTTGGAGAGCTTATCGGATCATTTCCAAGTTCGGTTTAGAATTGACGGTGTTTTA
CAAGCTCAGCCTCCACTTAGTAAAAATTTGGCTAATCGAATTATTTCTCGCCTAAAACTACTCGCTAAATTAGATATCAG
TGAAACCCGCCTTCCACAAGATGGGCGATTTCAATTTAAAACTACGTTTTCCGATATTCTTGATTTCCGTCTTTCAACAC
TCCCAACTCATTGGGGAGAGAAAGTTGTCCTGAGAGCACAGCAAAATAAACCAGTAGAACTCAGCTTTTCTGAACTGGGC
ATGACAGAGACTCAACAGCAAGCATTTCAACGCGCACTTAGCCAACCACAAGGATTAATTTTAGTAACCGGCCCAACAGG
AAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCCGAGGATC
CCATTGAAATTGAGCTTGATGGCATCATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTACGC
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCAATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAGATTGAAAACAGTTTGCTGCTCGTCATTGCTCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATACGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A502LH77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

92.967

98.06

0.912
  pilB Haemophilus influenzae Rd KW20

91.63

97.845

0.897
  pilB Glaesserella parasuis strain SC1401

58.772

98.276

0.578
  pilB Vibrio cholerae strain A1552

41.856

100

0.438
  pilB Vibrio campbellii strain DS40M4

40.862

100

0.429
  pilB Legionella pneumophila strain ERS1305867

39.798

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.748

100

0.422
  pilB Acinetobacter baylyi ADP1

38.076

100

0.409
  pilB Acinetobacter baumannii D1279779

39.337

100

0.409
  pilF Neisseria gonorrhoeae MS11

37.395

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.1

100

0.375
  pilF Thermus thermophilus HB27

35.789

100

0.366

Multiple sequence alignment