Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SPUTW3181_RS06890 Genome accession   NC_008750
Coordinates   1508477..1509589 (+) Length   370 a.a.
NCBI ID   WP_011788675.1    Uniprot ID   A0A6N3J369
Organism   Shewanella sp. W3-18-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1503477..1514589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPUTW3181_RS06855 (Sputw3181_1319) rdgB 1503572..1504189 (-) 618 WP_011788668.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SPUTW3181_RS06860 (Sputw3181_1320) - 1504300..1504734 (-) 435 WP_011788669.1 DUF4426 domain-containing protein -
  SPUTW3181_RS06865 (Sputw3181_1321) yggU 1504812..1505102 (-) 291 WP_011788670.1 DUF167 family protein YggU -
  SPUTW3181_RS06870 (Sputw3181_1322) - 1505102..1505650 (-) 549 WP_011788671.1 YggT family protein -
  SPUTW3181_RS06875 (Sputw3181_1323) proC 1505697..1506515 (-) 819 WP_011788672.1 pyrroline-5-carboxylate reductase -
  SPUTW3181_RS06880 (Sputw3181_1324) - 1506696..1507394 (-) 699 WP_011788673.1 YggS family pyridoxal phosphate-dependent enzyme -
  SPUTW3181_RS06885 (Sputw3181_1325) pilT 1507430..1508467 (+) 1038 WP_011788674.1 type IV pilus twitching motility protein PilT Machinery gene
  SPUTW3181_RS06890 (Sputw3181_1326) pilU 1508477..1509589 (+) 1113 WP_011788675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SPUTW3181_RS06895 (Sputw3181_1327) - 1509728..1510267 (+) 540 WP_011788676.1 glutathione peroxidase -
  SPUTW3181_RS06900 (Sputw3181_1328) hemH 1510300..1511313 (+) 1014 WP_011788677.1 ferrochelatase -
  SPUTW3181_RS06905 (Sputw3181_1329) ruvX 1511375..1511800 (-) 426 WP_011788678.1 Holliday junction resolvase RuvX -
  SPUTW3181_RS06910 (Sputw3181_1330) - 1511832..1512395 (-) 564 WP_011788679.1 YqgE/AlgH family protein -
  SPUTW3181_RS06915 (Sputw3181_1331) yciH 1512508..1512837 (-) 330 WP_011788680.1 stress response translation initiation inhibitor YciH -
  SPUTW3181_RS06920 (Sputw3181_1332) - 1513003..1513317 (-) 315 WP_011788681.1 hypothetical protein -
  SPUTW3181_RS06925 (Sputw3181_1333) - 1513496..1514056 (+) 561 WP_011788682.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41097.28 Da        Isoelectric Point: 6.3723

>NTDB_id=27176 SPUTW3181_RS06890 WP_011788675.1 1508477..1509589(+) (pilU) [Shewanella sp. W3-18-1]
MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKEPTGSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=27176 SPUTW3181_RS06890 WP_011788675.1 1508477..1509589(+) (pilU) [Shewanella sp. W3-18-1]
ATGGATGTACGTCCGTTTTTAAAAGTCATCGTTGAGCGTAAAGCCTCTGATTTGTTTATTACCGCGGGCTTTCCTCCCAG
TGCAAAAATTGATGGCGAATTACGTCCCCTCGCCGAGAGTGCATTTACGCCTGCACAGTCTTTAGATTTTGTTGAGTCAC
TCATGACTGAAGCGCAGAAACAGGAATTTCACTCAACCCGTGAATGTAACTTTGCTTTTGCGGCAAAGGATCTCGGTCGT
TTCCGGGTCAGCGCTTTTTGGCAAAGGGAATCTCCAGGATGTGTTATGCGCCGGATTGAGACCAAAATTCCCGAAGTCGA
TGACTTAAAATTACCTCCTATTTTAAAAGATCTGGTGATGAGCAAGCGTGGGCTTATCATTATGGTCGGCGGCACAGGTA
CGGGTAAGTCAACCTCATTAGCAGCATTGGTTGGATACCGTAATGCCCATGCCCGTGGCCATATATTGACGATTGAAGAT
CCTGTTGAATTTGTTCACGATCATAGAAAAAGCATTATCACCCAACGTGAAGTAGGCATAGATACCGAGTCATTCGATGC
GGCATTAAAAAGCTCCCTTCGTCAGGCGCCCGATGTGATCTTAATCGGTGAAATTCGTACCCAAGAGACGATGGAATTTG
CATTGTCGTTTGCAGAAACAGGTCATCTTTGTATGGCAACCCTACATGCCAATAATGCGAACCAAGCACTCGATCGGATT
ATGCACTTAGTGCCAGAGAGTAAACACCAGCAGTTACTATTTGATTTATCCTTAAATTTACGTGGTATTGTGGCGCAGCA
ATTGATCCCAAAAGTCGATGGAACGGGGCGCCGCGCCGCGATTGAAGTGTTAATTAATACTCCAAGGGTAGCGAGTTTAA
TTGCCAAAAATGAACTGCATTTACTCAAGGAAACCATGGCAAAATCGAATGAACAGGGGATGCAGACATTCGACCAAGCG
TTACTGAAATTATATGTTGATGGTGAAATTAGTTATGCCGATGCCTTACACCATGCTGACTCACCAAACGATTTACGTTT
GATGATCAAATTACAAAATAAAGAGCCTACTGGTTCAAGTTTTATGGAGGGCGTAACACTCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N3J369

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.215

100

0.605
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362

Multiple sequence alignment