Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   BKM03_RS05500 Genome accession   NZ_CP026562
Coordinates   1065006..1065434 (+) Length   142 a.a.
NCBI ID   WP_005736082.1    Uniprot ID   -
Organism   Pseudomonas avellanae strain R2leaf     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1060006..1070434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKM03_RS05475 (BKM03_05475) yacG 1060165..1060374 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  BKM03_RS05480 (BKM03_05480) coaE 1060371..1060994 (-) 624 WP_005736088.1 dephospho-CoA kinase -
  BKM03_RS05485 (BKM03_05485) pilD 1060991..1061863 (-) 873 WP_005736087.1 A24 family peptidase Machinery gene
  BKM03_RS05490 (BKM03_05490) pilC 1061865..1063082 (-) 1218 WP_005736085.1 type II secretion system F family protein Machinery gene
  BKM03_RS05495 (BKM03_05495) pilB 1063085..1064779 (-) 1695 WP_005736083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BKM03_RS05500 (BKM03_05500) pilA2 1065006..1065434 (+) 429 WP_005736082.1 pilin Machinery gene
  BKM03_RS05505 (BKM03_05505) - 1065597..1065962 (-) 366 WP_005736080.1 helix-turn-helix domain-containing protein -
  BKM03_RS05510 (BKM03_05510) gstA 1066120..1066740 (+) 621 WP_005736078.1 glutathione transferase GstA -
  BKM03_RS05515 (BKM03_05515) - 1066839..1067384 (-) 546 WP_003383227.1 DUF1523 family protein -
  BKM03_RS05520 (BKM03_05520) - 1067505..1068503 (+) 999 WP_005736077.1 ATP-grasp domain-containing protein -
  BKM03_RS05525 (BKM03_05525) - 1068558..1069706 (+) 1149 WP_005736076.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14439.66 Da        Isoelectric Point: 8.4580

>NTDB_id=270266 BKM03_RS05500 WP_005736082.1 1065006..1065434(+) (pilA2) [Pseudomonas avellanae strain R2leaf]
MKTQKGFTLIELMIVVAIVGILAAVAIPAYQDYTIRAQVAELVALTDGAKIAVSEAYQTTGAMPANSGAAGYGGASGTYT
ASVVIGANGVITATALGAAPVNATIRGQTFVMTPLPNGTNNTITWTCNTGTIIAQYRPAVCR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=270266 BKM03_RS05500 WP_005736082.1 1065006..1065434(+) (pilA2) [Pseudomonas avellanae strain R2leaf]
ATGAAAACACAAAAAGGTTTTACGTTGATCGAACTGATGATCGTCGTAGCGATCGTTGGCATTCTTGCAGCGGTAGCTAT
TCCTGCATATCAGGACTACACAATCCGTGCTCAGGTAGCAGAGTTGGTAGCACTCACGGATGGTGCGAAAATTGCCGTTA
GTGAAGCGTATCAGACCACTGGTGCTATGCCTGCCAACAGCGGCGCTGCTGGATATGGTGGTGCTTCTGGCACATACACT
GCAAGTGTTGTAATCGGAGCCAATGGAGTTATTACCGCGACAGCGCTTGGCGCGGCGCCAGTCAATGCTACAATTCGCGG
TCAAACTTTCGTTATGACCCCACTACCGAATGGCACGAACAACACTATCACTTGGACATGCAACACAGGGACAATCATCG
CTCAGTATCGTCCAGCGGTCTGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(36-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

59.286

98.592

0.585
  pilA2 Legionella pneumophila strain ERS1305867

57.857

98.592

0.57
  pilE Neisseria gonorrhoeae MS11

42.675

100

0.472
  pilE Neisseria gonorrhoeae strain FA1090

41.772

100

0.465
  pilA Ralstonia pseudosolanacearum GMI1000

39.053

100

0.465
  comP Acinetobacter baylyi ADP1

40

100

0.423
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.161

100

0.394
  pilA Acinetobacter baumannii strain A118

38.356

100

0.394

Multiple sequence alignment