Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BKM03_RS05490 Genome accession   NZ_CP026562
Coordinates   1061865..1063082 (-) Length   405 a.a.
NCBI ID   WP_005736085.1    Uniprot ID   A0A2S4IA24
Organism   Pseudomonas avellanae strain R2leaf     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1056865..1068082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKM03_RS05460 (BKM03_05460) - 1058217..1058750 (-) 534 WP_005736091.1 MOSC domain-containing protein -
  BKM03_RS05465 (BKM03_05465) - 1058747..1059376 (-) 630 WP_005736090.1 DUF1780 domain-containing protein -
  BKM03_RS05470 (BKM03_05470) - 1059573..1059899 (+) 327 WP_005736089.1 hypothetical protein -
  BKM03_RS05475 (BKM03_05475) yacG 1060165..1060374 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  BKM03_RS05480 (BKM03_05480) coaE 1060371..1060994 (-) 624 WP_005736088.1 dephospho-CoA kinase -
  BKM03_RS05485 (BKM03_05485) pilD 1060991..1061863 (-) 873 WP_005736087.1 A24 family peptidase Machinery gene
  BKM03_RS05490 (BKM03_05490) pilC 1061865..1063082 (-) 1218 WP_005736085.1 type II secretion system F family protein Machinery gene
  BKM03_RS05495 (BKM03_05495) pilB 1063085..1064779 (-) 1695 WP_005736083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BKM03_RS05500 (BKM03_05500) pilA2 1065006..1065434 (+) 429 WP_005736082.1 pilin Machinery gene
  BKM03_RS05505 (BKM03_05505) - 1065597..1065962 (-) 366 WP_005736080.1 helix-turn-helix domain-containing protein -
  BKM03_RS05510 (BKM03_05510) gstA 1066120..1066740 (+) 621 WP_005736078.1 glutathione transferase GstA -
  BKM03_RS05515 (BKM03_05515) - 1066839..1067384 (-) 546 WP_003383227.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43863.64 Da        Isoelectric Point: 10.1493

>NTDB_id=270264 BKM03_RS05490 WP_005736085.1 1061865..1063082(-) (pilC) [Pseudomonas avellanae strain R2leaf]
MASKAVKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPTKVRKKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVNSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVVVVAVIVSGILLIKVVPQFQSVFAGFGAELPGFTLMVIGLSEVVQKWWLAIVVAFFAGFFLFK
RAYKQSQNFRDGLDRFLLKIPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNKVKQDVSTGM
QLNFSMRATGVFPSLAIQMTAIGEESGALDNMLDKVATYYEDEVDNMVDSLTSLMEPMIMGVLGVIVGGLVIAMYLPIFK
LGGAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=270264 BKM03_RS05490 WP_005736085.1 1061865..1063082(-) (pilC) [Pseudomonas avellanae strain R2leaf]
ATGGCCAGCAAGGCAGTCAAAGTCATCGTTTACACGTGGGAAGGTGTAGACAAGAAAGGCACTAAAACCAGTGGCGAACT
GAGCGGCCATAATCTGGCACTCGTAAAAGCCCAATTGCGCAAGCAAGGCATCAACCCGACCAAGGTACGCAAGAAGTCTG
TATCAATCTTCGGCAAGGGCAAGAAAATCAAGCCACTGGATATCGCTTTTTTTTCTCGTCAGATGGCCACGATGATGAAA
GCAGGCGTCCCGCTGCTTCAGTCATTCGACATTATCAGTGAGGGCGCAGAAAATCCAAATATGCGGGCATTGGTCAACTC
GCTCAAGCAAGAAGTCTCGGCAGGCAATAGTTTTGCAACCGCCTTGCGACAGAAACCTGAGTATTTCGACGATCTTTTTT
GCAATCTGGTAGATGCGGGCGAACAAGCCGGGGCCCTGGAGAGCCTTTTAGATCGTGTTGCCAGCTATAAAGAAAAAACT
GAAAAACTCAAAGCAAAGATCAAAAAGGCCATGACCTACCCAGCGGCTGTAGTCGTAGTAGCCGTTATCGTGTCAGGTAT
CCTGCTCATAAAAGTAGTACCGCAATTCCAGTCAGTGTTCGCAGGGTTCGGTGCGGAGCTACCAGGATTTACGTTAATGG
TGATTGGCCTCTCAGAGGTCGTGCAAAAATGGTGGCTTGCAATTGTCGTTGCTTTTTTTGCTGGTTTTTTTCTTTTCAAG
AGAGCTTACAAGCAGTCCCAGAATTTTCGCGATGGACTGGATCGCTTTCTACTTAAAATACCGATTATTGGACCACTGAT
TTTCAAATCCTCGGTTGCACGTTACGCACGCACGCTAGCGACGACTTTCGCCGCTGGTGTTCCATTGGTTGAGGCGCTGG
ACTCTGTAGCCGGAGCGACTGGTAACGTGGTCTTTAGAAATGCGGTTAACAAAGTTAAGCAGGATGTTTCTACCGGCATG
CAGTTAAACTTTTCAATGCGCGCCACCGGGGTATTCCCTAGTTTGGCGATCCAAATGACCGCGATTGGAGAGGAGTCAGG
CGCGCTGGACAACATGCTCGATAAGGTCGCGACCTACTATGAAGATGAAGTCGACAACATGGTCGACAGTCTGACCAGTT
TGATGGAGCCGATGATCATGGGCGTTTTGGGCGTCATTGTGGGCGGCTTGGTAATTGCAATGTACCTGCCAATATTCAAA
CTTGGGGGCGCAGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S4IA24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

59.406

99.753

0.593
  pilC Legionella pneumophila strain ERS1305867

51.724

100

0.519
  pilC Vibrio cholerae strain A1552

42.643

99.012

0.422
  pilG Neisseria gonorrhoeae MS11

42.317

98.025

0.415
  pilC Vibrio campbellii strain DS40M4

42.602

96.79

0.412
  pilG Neisseria meningitidis 44/76-A

42.065

98.025

0.412
  pilC Thermus thermophilus HB27

39.506

100

0.395

Multiple sequence alignment