Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BKM03_RS05495 Genome accession   NZ_CP026562
Coordinates   1063085..1064779 (-) Length   564 a.a.
NCBI ID   WP_005736083.1    Uniprot ID   -
Organism   Pseudomonas avellanae strain R2leaf     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1058085..1069779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKM03_RS05460 (BKM03_05460) - 1058217..1058750 (-) 534 WP_005736091.1 MOSC domain-containing protein -
  BKM03_RS05465 (BKM03_05465) - 1058747..1059376 (-) 630 WP_005736090.1 DUF1780 domain-containing protein -
  BKM03_RS05470 (BKM03_05470) - 1059573..1059899 (+) 327 WP_005736089.1 hypothetical protein -
  BKM03_RS05475 (BKM03_05475) yacG 1060165..1060374 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  BKM03_RS05480 (BKM03_05480) coaE 1060371..1060994 (-) 624 WP_005736088.1 dephospho-CoA kinase -
  BKM03_RS05485 (BKM03_05485) pilD 1060991..1061863 (-) 873 WP_005736087.1 A24 family peptidase Machinery gene
  BKM03_RS05490 (BKM03_05490) pilC 1061865..1063082 (-) 1218 WP_005736085.1 type II secretion system F family protein Machinery gene
  BKM03_RS05495 (BKM03_05495) pilB 1063085..1064779 (-) 1695 WP_005736083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BKM03_RS05500 (BKM03_05500) pilA2 1065006..1065434 (+) 429 WP_005736082.1 pilin Machinery gene
  BKM03_RS05505 (BKM03_05505) - 1065597..1065962 (-) 366 WP_005736080.1 helix-turn-helix domain-containing protein -
  BKM03_RS05510 (BKM03_05510) gstA 1066120..1066740 (+) 621 WP_005736078.1 glutathione transferase GstA -
  BKM03_RS05515 (BKM03_05515) - 1066839..1067384 (-) 546 WP_003383227.1 DUF1523 family protein -
  BKM03_RS05520 (BKM03_05520) - 1067505..1068503 (+) 999 WP_005736077.1 ATP-grasp domain-containing protein -
  BKM03_RS05525 (BKM03_05525) - 1068558..1069706 (+) 1149 WP_005736076.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62461.73 Da        Isoelectric Point: 6.3854

>NTDB_id=270265 BKM03_RS05495 WP_005736083.1 1063085..1064779(-) (pilB) [Pseudomonas avellanae strain R2leaf]
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETSLATQNDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIREGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=270265 BKM03_RS05495 WP_005736083.1 1063085..1064779(-) (pilB) [Pseudomonas avellanae strain R2leaf]
ATGACTGATGCTGTCCTTACTGGCTTGGCCAAACAATTGGTCCTCGCCGAGCTCCTTACTGAACCTGTTGCGCAGCAAGC
GTATCAACAGGCGCGTCGCGACAAGATCTCGTTGGTGAGCTATCTGGTTCAGAACAAACTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCCGACCAGTTTGGCGTGCCGTTCATGGACCTCGCCAGCCTGGACAAGGAAAGCCAGCCCAAAGGCCTG
GTGAGTGAGAAGCTGGTTCGCCAGCATCACGCCCTGCCGCTGTGGCGACGCGGTAACAAGCTCTTTATCGGGATTTCCGA
CCCGACCAATCATCAGGCTGTGACCGATATTCAGTTCAGTACTGGCCTGAACACCGAAGCGATTCTGGTCGAAGATGACA
AGCTGACCACTGCGATAGATCGATTCTTTGACAGTGACAGCGGTCTGGGTAACTTGGAAGATGTCGATCTGGGCCTGGAT
ATCGAACCTGCTGATGGCAAGGAAACCTCCCTCGCGACGCAGAATGACGCCGACGACGCCCCCGTGGTCCGTTTCGTCAA
TAAAATGCTGCTGGATGCAATCCGGCTAGGATCATCCGATTTACATTTCGAACCCTACGAAAAGATCTTTCGCGTGCGCC
TACGCACCGACGGCATTTTGCATGAAGTTGCCAGGCCGCCGATTCACCTGGCAAACCGTATTGCAGCGCGCTTGAAAGTC
ATGGCAAGCCTCGATATCTCGGAGCGGCGCAAACCGCAGGACGGGCGGGTCAAGTTGCGGGTGTCCAAAACCAAGGCGAT
TGATTTTCGTATGAATACCTTGCCCACACTGTGGGGCGAGAAGATCGTGATGCGGATCCTGGACCCGACCAGCGCGCAGA
TGGGCATTGATGCTCTAGGCTACGAGCCAGAACAGAAAGCACTGTATCTGGAGGCGCTAAAACAGCCCCAGGGCATGATT
CTTGTGACTGGCCCCACGGGTTCCGGCAAGACCGTTTCCCTGTATACCGGTCTGAATATCCTTAATACGGTGGACATCAA
CATTTCCACAGCTGAAGACCCCGTGGAGATCAATCTGGAAGGTATTAATCAGGTCAACGTCAACCCGCGTCAGGGGCTGG
ACTTTTCGCAGGCATTGCGCGCCTTCTTGCGTCAAGATCCCGACGTGATCATGGTCGGCGAGATACGAGACTTGGAAACC
GCCGAAATCGCCATAAAGGCATCACAAACCGGCCACATGGTGCTGTCCACCCTGCACACCAACAGCGCTGCTGAAACCTT
GACGCGACTGCATCATATGGGCGTGGCCGCATTTAATATCGCCACTGCAATCAACCTGATTATTGCGCAACGGCTCGCGC
GCAAGCTGTGCAGCCATTGCAAGAAAGAACTCGATATTCCTCGCGAGACACTGATTCGGGAAGGTTTTCCAGAAGCGAAG
ATTGGCACTTTCAAGATCTATGGTCCGGTGGGCTGCGAACATTGCAATGGTGGCTATAGAGGCAGGGTCGGTATCTACGA
AGTCGTCAAGAAAACAGCAGAGCTGGAGCGCATCATCATGGAAGAGGGTAACTCACTGGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAACGACCTGCGAACCTCAGGCCTGTCAAAAGCCATGCAGGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.672

100

0.58
  pilB Acinetobacter baylyi ADP1

56.537

100

0.567
  pilB Legionella pneumophila strain ERS1305867

55.575

100

0.557
  pilB Vibrio cholerae strain A1552

50.89

99.645

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.626

99.113

0.502
  pilB Vibrio campbellii strain DS40M4

49.47

100

0.496
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

39.046

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.973

93.262

0.363

Multiple sequence alignment