Detailed information NTG0269489

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: DOM02_RS26985
• Genome accession: NZ_CP031677
• Coordinates: 5585548..5586021 (-)
• Length: 157 a.a.
• NCBI ID: WP_015503553.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain E80
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5580548..5591021

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DOM02_RS26945 (DOM02_26825)	-	5581028..5581690 (+)	663	WP_023082023.1	DUF3218 family protein	-
DOM02_RS26955 (DOM02_26835)	fliP	5581973..5582725 (+)	753	WP_023082025.1	flagellar type III secretion system pore protein FliP	-
DOM02_RS26960 (DOM02_26840)	fliQ	5582770..5583039 (+)	270	WP_023081841.1	flagellar biosynthesis protein FliQ	-
DOM02_RS26965 (DOM02_26845)	fliR	5583039..5583815 (+)	777	WP_003083054.1	flagellar biosynthetic protein FliR	-
DOM02_RS26970 (DOM02_26850)	-	5583818..5584077 (+)	260	Protein_5206	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
DOM02_RS26975 (DOM02_26855)	-	5585075..5585317 (+)	243	WP_023082027.1	hypothetical protein	-
DOM02_RS26985 (DOM02_26865)	pilE	5585548..5586021 (-)	474	WP_015503553.1	pilin	Machinery gene
DOM02_RS26990 (DOM02_26870)	pilB	5586252..5587952 (+)	1701	WP_003120945.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DOM02_RS26995 (DOM02_26875)	pilC	5587956..5589173 (+)	1218	WP_010792214.1	type II secretion system F family protein	Machinery gene
DOM02_RS27000 (DOM02_26880)	pilD	5589176..5590048 (+)	873	WP_003116251.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
DOM02_RS27005 (DOM02_26885)	coaE	5590045..5590656 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
DOM02_RS27010 (DOM02_26890)	yacG	5590653..5590853 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 157 a.a.
• Molecular weight: 16090.45 Da
• Isoelectric Point: 8.4684

>NTDB_id=269489 DOM02_RS26985 WP_015503553.1 5585548..5586021(-) (pilE) [Pseudomonas aeruginosa strain E80]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLRGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide

• Length: 474 bp

>NTDB_id=269489 DOM02_RS26985 WP_015503553.1 5585548..5586021(-) (pilE) [Pseudomonas aeruginosa strain E80]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

• Pilin (bacterial filament) (Pilin) (36-150)

• Prokaryotic N-terminal methylation motif (N_methyl) (4-27)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Neisseria gonorrhoeae strain FA1090	42.197	100	0.465
comP	Acinetobacter baylyi ADP1	47.059	97.452	0.459
pilE	Neisseria gonorrhoeae MS11	42.515	100	0.452
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	41.176	100	0.446
pilA/pilA1	Eikenella corrodens VA1	41.975	100	0.433
pilA2	Legionella pneumophila str. Paris	45.578	93.631	0.427
pilA2	Legionella pneumophila strain ERS1305867	44.218	93.631	0.414
pilA	Ralstonia pseudosolanacearum GMI1000	39.394	100	0.414
pilA/pilAI	Pseudomonas stutzeri DSM 10701	43.333	95.541	0.414
pilA	Pseudomonas aeruginosa PAK	41.722	96.178	0.401
pilA	Acinetobacter nosocomialis M2	55.357	71.338	0.395