Detailed information NTG0262456

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C0J56_RS11400
• Genome accession: NZ_CP025542
• Coordinates: 2474783..2475898 (+)
• Length: 371 a.a.
• NCBI ID: WP_109751850.1
• Uniprot ID: -
• Organism: Pseudomonas fluorescens strain 2P24
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2469783..2480898

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C0J56_RS11385 (C0J56_11385)	-	2470750..2471667 (+)	918	WP_134923750.1	sugar kinase	-
C0J56_RS11390 (C0J56_11390)	-	2471792..2472067 (-)	276	WP_019690952.1	peptidylprolyl isomerase	-
C0J56_RS11395 (C0J56_11395)	-	2472113..2474650 (-)	2538	WP_134923751.1	PAS domain-containing protein	-
C0J56_RS11400 (C0J56_11400)	pilU	2474783..2475898 (+)	1116	WP_109751850.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
C0J56_RS11405 (C0J56_11405)	-	2476064..2478019 (-)	1956	WP_134923752.1	acetoacetate--CoA ligase	-
C0J56_RS11410 (C0J56_11410)	-	2478177..2478953 (-)	777	WP_134923753.1	3-hydroxybutyrate dehydrogenase	-
C0J56_RS11415 (C0J56_11415)	-	2478961..2480352 (-)	1392	WP_134923754.1	GntP family permease	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41201.16 Da
• Isoelectric Point: 6.2506

>NTDB_id=262456 C0J56_RS11400 WP_109751850.1 2474783..2475898(+) (pilU) [Pseudomonas fluorescens strain 2P24]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVLKPLGNEACKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDQAL
FNLVAEGAIDEEEALKNADSANNLRLRLKLHAESGAAPPADPAAGEWGLMD

Nucleotide

• Length: 1116 bp

>NTDB_id=262456 C0J56_RS11400 WP_109751850.1 2474783..2475898(+) (pilU) [Pseudomonas fluorescens strain 2P24]
ATGGAAATCGATCCGTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGACCTCTACATGTCTACAGGGGCGCCGCCCTG
CGCGCGTTTCGAAGGCGTGCTCAAGCCCTTGGGTAACGAGGCATGCAAGGTCGGCGACATTGCCAGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAGTTGGAAATGAACCTGGCGATCTCCCTGGCTGGCGTCGGGCGT
TTCCGGGTCAATATCTTCAAGCAACGCAATGACGTGTCCATGGTGATACGCAACGTCAAGCTGGACATCCCGCGCTTCGA
AGACCTGAAACTGCCGCGGGTGCTGCTCGATACCATCATGCAGAAGCAAGGGCTGATGCTGTTCGTCGGGGCGACCGGGT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAACCAGCGCGAGGTCGGTGTCGACACCCGCAGTTTTCATGC
TGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAGATCCGCGACCGGGAAACCATGGAGCATG
CCTTGGCGTTTGCCGACACCGGGCACTTGGTGATTTCCACGTTGCATGCCCACAACGCCAACCAGGCCCTGGACCGGGTG
ATCAACTTCTTCCCGGAAGAGCGTCGGCCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAACG
CCTGGTGCGCACCCGTACCGGCCAGCGTCGGGCGGCGGTGGAGGTGATGCTGGGCTCGCCCACGGTAGCCGACCTGATCC
GCCGCAATGAGCTGGGCGAGCTCAAGGGCATCATGGAAAAGTCCGAAGAGTTGGGTATGCAGACCTTTGACCAGGCCTTG
TTCAACCTGGTGGCAGAGGGCGCCATCGATGAGGAAGAAGCGCTGAAAAACGCTGATTCGGCGAACAACCTGCGCTTGCG
CCTGAAGTTGCATGCCGAGTCGGGCGCGGCGCCACCCGCCGATCCGGCGGCGGGTGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	95.687	0.539
pilU	Acinetobacter baylyi ADP1	51.791	97.844	0.507
pilU	Vibrio cholerae strain A1552	52.857	94.34	0.499
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.692	98.922	0.383
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.757	0.369
pilT	Vibrio cholerae strain A1552	41.141	89.757	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.722	0.367
pilT	Acinetobacter baumannii D1279779	40.419	90.027	0.364
pilT	Acinetobacter baumannii strain A118	40.419	90.027	0.364
pilT	Pseudomonas aeruginosa PAK	39.244	92.722	0.364
pilT	Acinetobacter nosocomialis M2	40.12	90.027	0.361