Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CS390_RS01735 Genome accession   NZ_CP024478
Coordinates   383640..384872 (+) Length   410 a.a.
NCBI ID   WP_256587613.1    Uniprot ID   -
Organism   Pseudomonas sp. HLS-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 378640..389872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS390_RS01720 (CS390_01715) pilM 381191..382114 (+) 924 WP_065758205.1 type IV pilus biogenesis protein PilM -
  CS390_RS25030 (CS390_01720) - 382114..382656 (+) 543 WP_065758206.1 PilN domain-containing protein -
  CS390_RS01730 (CS390_01725) - 382653..383594 (+) 942 WP_099773335.1 pilus assembly protein PilP -
  CS390_RS01735 (CS390_01730) pilQ 383640..384872 (+) 1233 WP_256587613.1 type IV pilus secretin PilQ Machinery gene
  CS390_RS01740 (CS390_01735) aroK 384877..385395 (+) 519 WP_065758209.1 shikimate kinase AroK -
  CS390_RS01745 (CS390_01740) aroB 385490..386587 (+) 1098 WP_099773337.1 3-dehydroquinate synthase -
  CS390_RS01750 (CS390_01745) - 386604..388277 (+) 1674 WP_099773338.1 AAA family ATPase -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44239.56 Da        Isoelectric Point: 4.5660

>NTDB_id=253690 CS390_RS01735 WP_256587613.1 383640..384872(+) (pilQ) [Pseudomonas sp. HLS-6]
MFLVVADTDLRAAVSTGPYVGEPLSLNFQDVEVRSVLQVLADFTELNLVTSDAVQGTVTLRLQDVPWDHALDLVLRSKGL
GRRLDGNVLMVAPLEELAGLATLQREVLQVTHARAADIAELYLGLFTGDAAGAERGAIVIDERTNTILANQTSARLDALR
QLVAQLDVPVRQVLIEARIVEANVDYEKSFGIRWGGELSLGAGVALYGPGEVGRPFVDLGAERASSGIGLGLVRDSQLLD
LELTAMEKSGNGEIISQPKVVTADRETARILKGTEVPYQESGANGTTSITFREASLSLEVTPQIMADNRVVMAVKVTKDE
PDYLNAQAQVPPIRKNEVNAKVQVGDGETIVIGGVYSTVQSKVVDKVPFLGDVPYVGRLFKRDVVQEKKSELLVFLTPRI
MSDQAIAVSR

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=253690 CS390_RS01735 WP_256587613.1 383640..384872(+) (pilQ) [Pseudomonas sp. HLS-6]
TTGTTTTTAGTCGTGGCAGACACAGATCTGCGCGCGGCTGTATCGACAGGTCCTTATGTTGGAGAGCCATTGTCGCTGAA
CTTTCAGGATGTCGAGGTGCGTTCGGTGTTGCAGGTGCTGGCCGATTTCACCGAGCTGAATCTGGTAACCAGCGATGCGG
TGCAGGGCACTGTCACCCTGCGCTTGCAGGACGTGCCTTGGGATCACGCGTTGGACCTGGTGTTACGCAGCAAGGGGCTT
GGCAGGCGTCTGGACGGTAATGTGCTGATGGTGGCACCGCTGGAAGAATTGGCGGGGCTGGCGACATTGCAGCGTGAGGT
GTTGCAGGTCACGCATGCCAGGGCGGCAGATATCGCTGAGCTCTACCTGGGCTTGTTCACAGGGGATGCAGCAGGCGCTG
AGCGCGGTGCGATCGTGATTGATGAACGCACCAACACGATTCTCGCGAACCAGACTTCGGCGCGCCTTGACGCGTTGCGC
CAGCTCGTCGCGCAACTGGACGTGCCTGTGCGTCAGGTGCTGATCGAGGCAAGGATCGTTGAGGCCAATGTCGACTACGA
GAAAAGCTTTGGCATTCGCTGGGGTGGCGAGTTGAGCCTGGGTGCTGGCGTGGCGCTCTACGGGCCGGGTGAGGTGGGGC
GGCCTTTTGTCGACCTTGGGGCGGAACGGGCGAGCTCGGGCATCGGCTTGGGGCTGGTTCGTGACAGCCAATTGCTGGAC
CTTGAGCTCACTGCGATGGAAAAATCCGGTAACGGGGAGATCATCTCGCAGCCCAAGGTGGTGACTGCTGACCGAGAAAC
TGCACGGATCCTCAAGGGGACCGAGGTGCCTTATCAGGAGTCGGGCGCCAATGGCACCACCTCGATCACCTTTCGCGAGG
CGTCCCTGTCACTTGAAGTGACGCCGCAGATCATGGCGGACAACCGTGTGGTGATGGCCGTCAAGGTGACCAAGGATGAG
CCTGATTACCTCAATGCCCAGGCTCAGGTGCCGCCGATACGGAAGAATGAGGTCAACGCCAAGGTTCAGGTGGGTGATGG
GGAGACCATCGTGATTGGCGGGGTGTATTCGACGGTGCAAAGTAAAGTGGTGGATAAGGTGCCATTTTTGGGCGATGTGC
CGTATGTTGGGCGGCTGTTTAAGCGCGATGTCGTACAAGAAAAAAAATCTGAGCTGCTGGTCTTCCTGACTCCGCGTATC
ATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(245-401)

Secretin and TonB N terminus short domain (STN)

(48-93)

Bacterial type II/III secretion system short domain (Secretin_N)

(106-172)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

56.009

100

0.637
  comQ Acinetobacter baylyi ADP1

42.506

100

0.463
  pilQ Acinetobacter baumannii D1279779

38.947

100

0.451
  pilQ Acinetobacter baumannii strain A118

38.947

100

0.451
  pilQ Legionella pneumophila strain ERS1305867

39.408

100

0.422
  pilQ Legionella pneumophila strain Lp02

39.408

100

0.422
  pilQ Vibrio campbellii strain DS40M4

39.151

100

0.405
  pilQ Vibrio cholerae strain A1552

39.183

100

0.398
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.183

100

0.398
  pilQ Neisseria meningitidis M1080

35.238

100

0.361
  pilQ Neisseria meningitidis MC58

35.238

100

0.361

Multiple sequence alignment