Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PFL01_RS24250 Genome accession   NC_007492
Coordinates   5441417..5442634 (+) Length   405 a.a.
NCBI ID   WP_011335980.1    Uniprot ID   Q3K6P6
Organism   Pseudomonas fluorescens Pf0-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5436417..5447634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFL01_RS24230 (Pfl01_4817) - 5437943..5438239 (+) 297 WP_011335976.1 DUF2845 domain-containing protein -
  PFL01_RS24235 (Pfl01_4818) - 5438406..5438762 (-) 357 WP_011335977.1 BON domain-containing protein -
  PFL01_RS24240 (Pfl01_4819) - 5439071..5439487 (-) 417 WP_011335978.1 pilin -
  PFL01_RS24245 (Pfl01_4820) pilB 5439714..5441414 (+) 1701 WP_011335979.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PFL01_RS24250 (Pfl01_4821) pilC 5441417..5442634 (+) 1218 WP_011335980.1 type II secretion system F family protein Machinery gene
  PFL01_RS24255 (Pfl01_4822) pilD 5442636..5443508 (+) 873 WP_011335981.1 prepilin peptidase Machinery gene
  PFL01_RS24260 (Pfl01_4823) coaE 5443505..5444128 (+) 624 WP_011335982.1 dephospho-CoA kinase -
  PFL01_RS24265 (Pfl01_4824) yacG 5444125..5444325 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -
  PFL01_RS24270 (Pfl01_4825) - 5444332..5444547 (-) 216 WP_007959584.1 hypothetical protein -
  PFL01_RS24275 (Pfl01_4826) - 5444613..5445302 (-) 690 WP_011335984.1 energy-coupling factor ABC transporter permease -
  PFL01_RS24280 (Pfl01_4827) - 5445299..5445766 (-) 468 WP_011335985.1 hypothetical protein -
  PFL01_RS24285 (Pfl01_4828) - 5445897..5446523 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -
  PFL01_RS24290 (Pfl01_4829) - 5446520..5447047 (+) 528 WP_011335986.1 MOSC domain-containing protein -
  PFL01_RS24295 (Pfl01_4830) - 5447099..5447272 (+) 174 WP_003228304.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44104.76 Da        Isoelectric Point: 10.1093

>NTDB_id=24842 PFL01_RS24250 WP_011335980.1 5441417..5442634(+) (pilC) [Pseudomonas fluorescens Pf0-1]
MAVKAAKISVYAWEGTDRKGSKVTGELSGQNPALVKAQLRKQGINPGKVRKKSASLLSFGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ESLKAKIKKAMTYPSAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSEFMQQWWWAILGLLVASFFGTR
HALKKSQALRDRRDAWFLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRSTGIFPNMAVQMTAIGEESGALDEMLDKVAGFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=24842 PFL01_RS24250 WP_011335980.1 5441417..5442634(+) (pilC) [Pseudomonas fluorescens Pf0-1]
ATGGCCGTCAAGGCAGCGAAAATCAGTGTCTATGCCTGGGAAGGCACGGACCGCAAAGGCAGCAAGGTGACCGGCGAGCT
GAGCGGCCAGAACCCCGCGCTGGTCAAGGCCCAATTGCGCAAGCAGGGCATCAACCCCGGCAAGGTGCGCAAGAAATCCG
CCTCGTTGCTGAGCTTCGGCAAGCGCATCAAGGCCCAGGACATTGCCCTCTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCGTTCGACATCATTGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAGCTGGTCGACGA
GGTGAAACAGGAGGTCGCCGCCGGTAACAGTTTCGCCGCCGCGCTGCGCAAGAAACCGCAATATTTCGACGAGTTGTACT
GCAACCTGGTGGATGCCGGCGAGCAGTCCGGCGCCCTCGACACCCTGCTGGAGCGGGTCGCGACCTACAAAGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAGGCCATGACCTACCCGTCGGCCGTGGTGCTGGTGGCAGCGGTGGTTACCGGGAT
TCTGCTGGTCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCTCTGGCTTCGGTGCCGAACTGCCAGCCTTCACGTTGATGG
TGATCAGCCTGTCCGAATTCATGCAGCAATGGTGGTGGGCGATTCTCGGTTTGCTGGTGGCCAGTTTTTTCGGCACCCGC
CATGCCCTGAAAAAATCCCAGGCGCTGCGCGACCGGCGTGACGCCTGGTTTCTCAAGCTGCCGCTGGTGGGCACATTGAT
GTACAAGTCCGCCGTGGCGCGGTTTGCCCGGACCCTGTCGACCACCTTCGCGGCCGGCGTGCCACTGGTGGAAGCACTGG
ATTCGGTGGCCGGGGCCACCGGCAACGTAGTGTTCAAGCGCGCCGTGTTGCGCATCCGTCAGGACGTTTCCACCGGCATG
CAGCTGAATTTCTCGATGCGCTCCACCGGCATCTTTCCGAACATGGCGGTGCAGATGACCGCCATCGGCGAAGAGTCCGG
CGCACTGGACGAGATGCTCGACAAGGTCGCAGGTTTCTATGAGGACGAGGTGGACAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTCGGGGTGATCGTCGGCGGTCTGGTGGTGGCGATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3K6P6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.062

100

0.751
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

59.069

100

0.595
  pilC Legionella pneumophila strain ERS1305867

54.25

98.765

0.536
  pilC Vibrio cholerae strain A1552

44.192

97.778

0.432
  pilG Neisseria meningitidis 44/76-A

42.857

100

0.43
  pilG Neisseria gonorrhoeae MS11

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Thermus thermophilus HB27

39.152

99.012

0.388

Multiple sequence alignment