Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PFL01_RS24245 Genome accession   NC_007492
Coordinates   5439714..5441414 (+) Length   566 a.a.
NCBI ID   WP_011335979.1    Uniprot ID   -
Organism   Pseudomonas fluorescens Pf0-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5434714..5446414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFL01_RS24225 (Pfl01_4816) - 5434992..5437943 (+) 2952 WP_011335975.1 DUF748 domain-containing protein -
  PFL01_RS24230 (Pfl01_4817) - 5437943..5438239 (+) 297 WP_011335976.1 DUF2845 domain-containing protein -
  PFL01_RS24235 (Pfl01_4818) - 5438406..5438762 (-) 357 WP_011335977.1 BON domain-containing protein -
  PFL01_RS24240 (Pfl01_4819) - 5439071..5439487 (-) 417 WP_011335978.1 pilin -
  PFL01_RS24245 (Pfl01_4820) pilB 5439714..5441414 (+) 1701 WP_011335979.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PFL01_RS24250 (Pfl01_4821) pilC 5441417..5442634 (+) 1218 WP_011335980.1 type II secretion system F family protein Machinery gene
  PFL01_RS24255 (Pfl01_4822) pilD 5442636..5443508 (+) 873 WP_011335981.1 prepilin peptidase Machinery gene
  PFL01_RS24260 (Pfl01_4823) coaE 5443505..5444128 (+) 624 WP_011335982.1 dephospho-CoA kinase -
  PFL01_RS24265 (Pfl01_4824) yacG 5444125..5444325 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -
  PFL01_RS24270 (Pfl01_4825) - 5444332..5444547 (-) 216 WP_007959584.1 hypothetical protein -
  PFL01_RS24275 (Pfl01_4826) - 5444613..5445302 (-) 690 WP_011335984.1 energy-coupling factor ABC transporter permease -
  PFL01_RS24280 (Pfl01_4827) - 5445299..5445766 (-) 468 WP_011335985.1 hypothetical protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62667.69 Da        Isoelectric Point: 5.9781

>NTDB_id=24841 PFL01_RS24245 WP_011335979.1 5439714..5441414(+) (pilB) [Pseudomonas fluorescens Pf0-1]
MNDIALSGLAKQLVQAELLTEKSAQHAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEHFGMAFLDLNCLDRETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
LDVESIDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSQCKKAIEIPRETLLKEGFPE
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTRMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=24841 PFL01_RS24245 WP_011335979.1 5439714..5441414(+) (pilB) [Pseudomonas fluorescens Pf0-1]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTACAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCACGC
CTGGCAGCAGGCCCAGCGCAATCGCCTGTCGCTGGTCAGCTATCTGGTACAAAACAAACTGGTGAAGAGCGCGCAGGTCG
CCGAGATTGCCTCGGAGCACTTCGGCATGGCGTTCCTCGACCTCAACTGTCTCGACAGGGAAACCCAGCCCAAGGGTCTG
GTCAGTGAAAAACTGGTGCGCCAGCACCATGCCCTGCCCCTGTGGCGTCGCGGCAACAAGCTGTTCGTGGGGATCTCCGA
CCCGAGCAATCATCAGGCGATCAACGACATCCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGACGCCATCGAGAAATTCTTCGACACCCACGCCTCCGGCCTGGAAGACATGGCCGATGTCGACCTCGACGGG
CTGGACGTCGAGTCCATCGATGACAGCAAGCAGGACGCCATCGGCGGGCTCGATGCCGACGATGCGCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTGGACGCAATCAAGAGCGGCTCTTCCGACCTGCACTTCGAGCCTTACGAGAAAAACTACCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAAGTGGCCAAGCCACCGATTCAATTGGCAGGACGCATCGCCGCACGCCTG
AAGGTGATGGCCAGCCTCGACATCTCGGAGCGCCGCAAACCTCAGGACGGGCGCCTGAAGATGCGCCTGTCAAAGACCAA
ATCGATCGATTTCCGGGTCAACACCCTGCCAACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCCTCCAGCG
CGCAGATCGGCATCGACGCCCTCGGTTATGAGCCGGAGCAGAAAGATCTGTACATGGCCGCCCTGAAACAGCCGCAAGGA
ATGATTCTGGTCACCGGCCCCACCGGTTCGGGCAAGACCGTTTCGCTGTACACCGGGCTGAATATCCTCAATACCGTCGA
CATCAACATCTCTACCGCTGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAAG
GGATGGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGTGACCTC
GAGACCGCCGAGATCGCCATCAAAGCAGCGCAGACCGGTCACCTCGTGCTCTCCACGCTGCACACCAACAGCGCCGCCGA
GACACTCACCCGCTTGCACAACATGGGCATTCCCGGCTTCAACATCGCCACGTCGGTGAGCCTGATCATCGCCCAGCGTC
TGGCGCGAAAACTCTGCAGCCAATGCAAGAAAGCCATCGAGATTCCCCGCGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCAGTTTCACAATCTATGAGCCCGTCGGCTGCGATCACTGCAACGGCGGGTACAAGGGTCGCGTGGGGAT
TTATGAAGTGGTGAAGAACACTCCCGAGTTGCAACGGCTGATCATGGCCGAGGGCAACTCACTGGAAATCGATACCCGGA
TGCGCCGGGACGGCTTCAACGACCTGCGCACCTCGGGCCTGATCAAAGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.053

99.823

0.58
  pilB Acinetobacter baumannii D1279779

57.801

99.647

0.576
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.558
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilF Neisseria gonorrhoeae MS11

49.734

99.47

0.495
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.466

99.293

0.491
  pilF Thermus thermophilus HB27

36.559

98.587

0.36
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.636

93.286

0.36

Multiple sequence alignment