Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PRUTH_RS12710 Genome accession   NZ_CP023396
Coordinates   2734629..2735858 (+) Length   409 a.a.
NCBI ID   WP_045979234.1    Uniprot ID   A0A0F4PQW9
Organism   Pseudoalteromonas ruthenica strain LMG 19699     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2729629..2740858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRUTH_RS12690 trmL 2730587..2731051 (+) 465 WP_022945433.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  PRUTH_RS12695 nadC 2731100..2731951 (+) 852 WP_151173422.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PRUTH_RS12700 - 2732329..2732787 (+) 459 WP_045979236.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PRUTH_RS12705 pilB 2732857..2734548 (+) 1692 WP_151173423.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PRUTH_RS12710 pilC 2734629..2735858 (+) 1230 WP_045979234.1 type II secretion system F family protein Machinery gene
  PRUTH_RS12715 pilD 2735883..2736755 (+) 873 WP_151173424.1 A24 family peptidase Machinery gene
  PRUTH_RS12720 coaE 2736763..2737368 (+) 606 WP_151173425.1 dephospho-CoA kinase -
  PRUTH_RS12725 pilB 2737457..2739136 (+) 1680 WP_151173426.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PRUTH_RS12730 yacG 2739214..2739453 (+) 240 WP_151173427.1 DNA gyrase inhibitor YacG -
  PRUTH_RS12735 - 2739462..2740241 (-) 780 WP_371741513.1 YggN family protein -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44432.13 Da        Isoelectric Point: 9.5785

>NTDB_id=246641 PRUTH_RS12710 WP_045979234.1 2734629..2735858(+) (pilC) [Pseudoalteromonas ruthenica strain LMG 19699]
MAKQQQANQLDTFQWVGVSTRGKKLEGELTGTSIALVKAQLRKQGITPSKVKRKPKPLFGLSGEKKISAKDIAMFTRQLA
TMLLAGVSLVQAIDMIADGSENKSVKNLLRSIGEEVKAGQPLSTALRKHPKYFDDLYCDLVASGEQSGALDRIFDRVALY
KEKAEALKSKIKKAMFYPVAVIFVAAIVTSILLIFVVPQFKEIFEGFGAELPAFTLLVISISEFMQAYWWIAVLVLGIGG
YVFKQAHRRNKQLRDNVDKLILKTPIVGSILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRDAILDIKAEV
SSGNPMNWAMRNSNIFPDMVIQMVAIGEESGSLDDMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLIGGLIIAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=246641 PRUTH_RS12710 WP_045979234.1 2734629..2735858(+) (pilC) [Pseudoalteromonas ruthenica strain LMG 19699]
ATGGCAAAACAACAACAAGCAAACCAATTGGATACTTTCCAGTGGGTGGGTGTAAGCACTCGTGGCAAAAAGCTTGAAGG
TGAACTAACCGGTACCAGTATCGCCTTAGTAAAAGCCCAATTGCGCAAGCAAGGGATCACACCTTCTAAAGTTAAACGCA
AACCCAAACCTCTATTTGGACTTTCTGGCGAGAAGAAGATATCAGCTAAAGATATTGCTATGTTTACCCGGCAACTAGCT
ACCATGTTGCTTGCAGGGGTGTCCTTGGTTCAGGCCATAGATATGATTGCCGATGGTTCTGAGAACAAGAGCGTTAAAAA
TCTCCTTCGCAGTATCGGAGAGGAAGTAAAGGCAGGCCAACCTCTGAGTACAGCATTACGTAAACATCCCAAATATTTTG
ATGATTTGTATTGCGATTTAGTTGCATCTGGTGAGCAGTCAGGTGCGCTAGATAGAATTTTTGACCGAGTCGCTCTTTAC
AAAGAAAAAGCAGAAGCTCTTAAATCGAAAATCAAAAAGGCGATGTTTTACCCTGTAGCCGTAATATTCGTAGCGGCAAT
CGTAACTTCTATATTGCTGATTTTTGTGGTGCCCCAGTTTAAAGAAATATTCGAGGGCTTTGGCGCCGAACTCCCTGCCT
TTACTCTTTTAGTGATCAGTATTTCAGAGTTTATGCAAGCATACTGGTGGATCGCTGTATTGGTACTGGGCATAGGTGGT
TACGTTTTCAAACAAGCACATCGACGCAATAAACAGCTACGAGATAATGTTGATAAGCTCATTCTGAAAACTCCTATCGT
TGGTTCTATCTTAAATAAGGCAGCTGTGGCGCGTTATGCTCGAACTTTATCGACTACTTTTGCCGCAGGTGTACCTTTAG
TGGATGCACTAGATTCAGCAGCAGGAGCTTCAGGTAATGCGGTTTATCGCGATGCGATTTTAGATATTAAAGCTGAGGTC
AGCTCTGGTAACCCAATGAACTGGGCAATGCGTAACTCTAATATCTTTCCCGATATGGTGATTCAAATGGTCGCCATTGG
TGAAGAGTCAGGTTCACTGGATGATATGCTTGCTAAGGTGGCAACCATTTATGAGCAAGAAGTAGACGATGCCGTGGACG
GCCTCTCAAGCCTGTTAGAGCCACTCATTATGGCTGTGCTAGGGGTGCTAATCGGTGGCCTTATTATCGCCATGTACCTG
CCTATATTCCAACTCGGTTCTGTTATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F4PQW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.741

99.022

0.601
  pilC Acinetobacter baylyi ADP1

56.373

99.756

0.562
  pilC Acinetobacter baumannii D1279779

54.568

99.022

0.54
  pilC Legionella pneumophila strain ERS1305867

54.75

97.8

0.535
  pilC Vibrio cholerae strain A1552

45.122

100

0.452
  pilC Vibrio campbellii strain DS40M4

43.596

99.267

0.433
  pilG Neisseria meningitidis 44/76-A

45.013

95.599

0.43
  pilG Neisseria gonorrhoeae MS11

44.757

95.599

0.428
  pilC Thermus thermophilus HB27

37.376

98.778

0.369

Multiple sequence alignment