Detailed information NTG0246642

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PRUTH_RS12715
• Genome accession: NZ_CP023396
• Coordinates: 2735883..2736755 (+)
• Length: 290 a.a.
• NCBI ID: WP_151173424.1
• Uniprot ID: -
• Organism: Pseudoalteromonas ruthenica strain LMG 19699
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2730883..2741755

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PRUTH_RS12695	nadC	2731100..2731951 (+)	852	WP_151173422.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PRUTH_RS12700	-	2732329..2732787 (+)	459	WP_045979236.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
PRUTH_RS12705	pilB	2732857..2734548 (+)	1692	WP_151173423.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PRUTH_RS12710	pilC	2734629..2735858 (+)	1230	WP_045979234.1	type II secretion system F family protein	Machinery gene
PRUTH_RS12715	pilD	2735883..2736755 (+)	873	WP_151173424.1	A24 family peptidase	Machinery gene
PRUTH_RS12720	coaE	2736763..2737368 (+)	606	WP_151173425.1	dephospho-CoA kinase	-
PRUTH_RS12725	pilB	2737457..2739136 (+)	1680	WP_151173426.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PRUTH_RS12730	yacG	2739214..2739453 (+)	240	WP_151173427.1	DNA gyrase inhibitor YacG	-
PRUTH_RS12735	-	2739462..2740241 (-)	780	WP_371741513.1	YggN family protein	-
PRUTH_RS12740	-	2740365..2741183 (-)	819	WP_257220938.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31664.82 Da
• Isoelectric Point: 7.5993

>NTDB_id=246642 PRUTH_RS12715 WP_151173424.1 2735883..2736755(+) (pilD) [Pseudoalteromonas ruthenica strain LMG 19699]
MKSEPWFFITTVLFVSLAVGSFLNVVIYRIPKMMQNQWQSECRLLLADELASKSEQSQSASAPFNLLTPASTCPKCNNKI
KPWHNIPLLSWLFLKGQCAYCQAPISIRYPAIELITAVLSLVVALTFGPTQLTVLYIIITWVLVALTFIDIDHMLLPDQL
TLPLLWLALLASVAGITIEPSAALIGAAAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAVFGALLGWQALLMIILLSSIV
GAVIGITLLSVQGKDKATPIPFGPYIAIAGWIAMLWGEQIQGAYFGAILG

Nucleotide

• Length: 873 bp

>NTDB_id=246642 PRUTH_RS12715 WP_151173424.1 2735883..2736755(+) (pilD) [Pseudoalteromonas ruthenica strain LMG 19699]
ATGAAAAGCGAGCCGTGGTTTTTTATAACCACGGTTCTTTTTGTATCCTTAGCTGTAGGTAGCTTCCTCAATGTGGTTAT
TTACCGCATTCCAAAGATGATGCAAAATCAATGGCAGTCAGAGTGTCGCTTACTACTTGCCGATGAATTGGCATCAAAAA
GTGAACAAAGCCAATCAGCATCCGCTCCTTTTAACCTTCTAACGCCAGCTTCGACTTGCCCGAAATGTAATAACAAAATC
AAGCCTTGGCACAATATCCCCCTGCTCAGTTGGCTATTTTTAAAGGGCCAATGCGCTTACTGCCAGGCGCCTATCTCTAT
TCGCTATCCAGCTATAGAGCTCATCACTGCGGTTCTAAGTTTAGTTGTCGCGCTTACCTTTGGCCCCACTCAACTAACCG
TGCTTTACATCATTATCACCTGGGTATTGGTGGCACTGACATTCATAGACATAGACCATATGCTGTTGCCAGATCAGCTG
ACGTTACCGCTGTTATGGCTGGCTTTATTAGCAAGTGTTGCAGGGATCACCATAGAACCGAGTGCAGCACTAATTGGCGC
TGCCGCAGGCTACCTTAGCTTATGGAGCGTTTATTGGCTGTTCAAATTGCTGACCGGTAAAGAAGGGATGGGCTATGGTG
ACTTTAAACTACTGGCGGTATTTGGTGCCTTATTAGGTTGGCAAGCATTACTGATGATCATACTCCTGTCTAGCATTGTG
GGTGCCGTAATTGGGATTACCTTACTCTCTGTTCAAGGCAAAGATAAGGCAACCCCGATTCCATTTGGGCCTTATATTGC
CATTGCTGGCTGGATAGCCATGCTTTGGGGAGAGCAAATCCAAGGCGCTTATTTCGGCGCTATTTTGGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (15-127)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.962	98.966	0.524
pilD	Vibrio campbellii strain DS40M4	52.265	98.966	0.517
pilD	Acinetobacter nosocomialis M2	53.986	95.172	0.514
pilD	Acinetobacter baumannii D1279779	53.986	95.172	0.514
pilD	Neisseria gonorrhoeae MS11	46.512	88.966	0.414