Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PRUTH_RS12705 Genome accession   NZ_CP023396
Coordinates   2732857..2734548 (+) Length   563 a.a.
NCBI ID   WP_151173423.1    Uniprot ID   -
Organism   Pseudoalteromonas ruthenica strain LMG 19699     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2727857..2739548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRUTH_RS12670 - 2727938..2728228 (-) 291 WP_022945437.1 cell division protein ZapA -
  PRUTH_RS12675 zapB 2728228..2728458 (-) 231 WP_045979239.1 cell division protein ZapB -
  PRUTH_RS12680 - 2728706..2729545 (+) 840 WP_151173421.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
  PRUTH_RS12685 - 2729558..2730496 (-) 939 WP_179954346.1 cation diffusion facilitator family transporter -
  PRUTH_RS12690 trmL 2730587..2731051 (+) 465 WP_022945433.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  PRUTH_RS12695 nadC 2731100..2731951 (+) 852 WP_151173422.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PRUTH_RS12700 - 2732329..2732787 (+) 459 WP_045979236.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PRUTH_RS12705 pilB 2732857..2734548 (+) 1692 WP_151173423.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PRUTH_RS12710 pilC 2734629..2735858 (+) 1230 WP_045979234.1 type II secretion system F family protein Machinery gene
  PRUTH_RS12715 pilD 2735883..2736755 (+) 873 WP_151173424.1 A24 family peptidase Machinery gene
  PRUTH_RS12720 coaE 2736763..2737368 (+) 606 WP_151173425.1 dephospho-CoA kinase -
  PRUTH_RS12725 pilB 2737457..2739136 (+) 1680 WP_151173426.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PRUTH_RS12730 yacG 2739214..2739453 (+) 240 WP_151173427.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62273.33 Da        Isoelectric Point: 5.3115

>NTDB_id=246640 PRUTH_RS12705 WP_151173423.1 2732857..2734548(+) (pilB) [Pseudoalteromonas ruthenica strain LMG 19699]
MNLNSPLARRLASSGAIDIQKLHSHKLDNPATLPEVLCQLSGLTPQQLQAKACHIFNVSQFDISQIDSEFITNTEFLNEK
LIRKHKTLPISVKGKTLYVATADPTNFSAFEDFEFNTGMQVEPIVVRYDQLDSVIDGLFDSASSLGLTEDDMDELEGVDS
GDGNKKDLATDVNSKEDDAPIIVYINKILMDAIKRGASDLHFEPYEKRYRIRFRVDGVLHEIASPPINLESRIAARIKVM
SHMDLAEKRKPQDGRIKLKINAKKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKQLYLDALAQPQGMIL
VTGPIGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGINQVQISAKSDMTFANALRAFLRQDPDIVMVGEIRDLETA
EISIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPSYNIASSVSLIIAQRLARRLCKHCKEPEQLPQEELIKQGFTQEQL
NDLTVYKPVGCDDCTEGYKGRVGIYEVMPITPKIAEIIMRGGNSLEIAEVATQEGVNNLRKSGLKKVAQGVTSLVEVNRV
TNV

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=246640 PRUTH_RS12705 WP_151173423.1 2732857..2734548(+) (pilB) [Pseudoalteromonas ruthenica strain LMG 19699]
ATGAACCTAAATTCTCCCTTAGCCCGCAGACTCGCCAGTTCTGGAGCTATAGATATACAAAAGCTCCATTCCCACAAACT
GGATAACCCAGCCACACTTCCTGAAGTTTTATGTCAGCTGTCAGGGTTAACGCCGCAGCAACTTCAAGCAAAAGCCTGCC
ATATTTTCAATGTGTCGCAGTTTGATATATCTCAGATCGATAGCGAATTCATCACAAACACCGAGTTTCTAAATGAAAAG
TTAATTCGGAAGCATAAAACGCTACCGATTTCGGTAAAGGGCAAAACCCTTTATGTCGCAACGGCCGACCCTACGAACTT
TAGCGCATTCGAAGATTTCGAATTTAATACAGGTATGCAGGTAGAGCCTATTGTCGTGCGTTATGATCAGCTCGACTCGG
TTATTGATGGTCTTTTTGACAGCGCCTCGAGCTTGGGGCTCACTGAAGACGATATGGACGAGCTCGAAGGTGTCGACTCT
GGTGATGGTAATAAGAAAGATTTAGCTACCGATGTAAACTCTAAGGAAGACGACGCCCCCATTATCGTTTACATCAATAA
AATTTTAATGGATGCGATTAAGCGCGGTGCTTCCGATTTACACTTTGAGCCTTATGAAAAACGTTACCGCATCCGTTTTC
GTGTTGATGGGGTTCTTCATGAAATAGCTTCCCCCCCAATCAACCTGGAAAGCCGTATCGCTGCACGTATCAAGGTTATG
TCGCATATGGACTTGGCGGAGAAGCGAAAACCTCAAGATGGCCGTATAAAATTAAAAATAAACGCCAAAAAAAGTATCGA
TTTCCGTGTGAGTACCCTCCCCACCCTTTGGGGTGAGAAAATCGTAATGCGTATTCTCGATAGTTCCAGTGCAATGCTTG
GCATTGATGTGCTTGGCTATGAGCCTGAGCAAAAACAGCTTTATTTGGATGCACTTGCACAACCTCAGGGAATGATTTTA
GTTACTGGCCCGATAGGTTCAGGTAAAACCGTATCACTTTATACCGGTCTCAATATTCTTAATACCACTGAGCGCAATAT
TAGTACTGCCGAAGACCCCGTTGAAATAAACCTTGAGGGGATTAACCAGGTTCAAATCAGCGCTAAATCTGATATGACCT
TTGCCAATGCTTTGCGTGCGTTTTTGCGTCAAGACCCTGATATTGTCATGGTGGGTGAGATTCGAGACTTAGAAACAGCG
GAAATATCGATAAAAGCAGCGCAAACAGGCCACTTAGTACTATCGACACTACACACCAACTCAGCACCAGAAACCTTGAC
TCGTTTAATGAATATGGGTGTACCGTCTTACAACATCGCCAGTTCAGTGAGCTTGATTATTGCCCAGCGTCTAGCTCGTC
GGTTATGCAAGCATTGCAAAGAGCCAGAACAGCTACCTCAAGAAGAACTGATAAAGCAAGGCTTTACACAAGAGCAGCTA
AACGATTTAACTGTCTACAAACCTGTAGGTTGTGATGATTGCACCGAAGGCTACAAAGGCCGAGTTGGTATTTATGAAGT
GATGCCCATTACTCCGAAAATCGCCGAAATTATTATGCGTGGCGGTAACTCATTAGAGATAGCAGAAGTCGCAACTCAAG
AGGGTGTCAATAACCTAAGAAAGTCAGGCTTAAAAAAAGTGGCACAGGGCGTGACTAGCCTAGTAGAAGTCAATCGCGTA
ACCAATGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-144)

Type II/IV secretion system protein (T2SSE)

(187-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.707

90.409

0.549
  pilB Acinetobacter baylyi ADP1

62.91

86.679

0.545
  pilB Legionella pneumophila strain ERS1305867

54.025

99.29

0.536
  pilB Vibrio cholerae strain A1552

52.974

95.56

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

53.283

94.671

0.504
  pilB Vibrio campbellii strain DS40M4

52.612

95.204

0.501
  pilF Neisseria gonorrhoeae MS11

50.382

93.073

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

37.338

100

0.409

Multiple sequence alignment