Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PRUTH_RS12725 Genome accession   NZ_CP023396
Coordinates   2737457..2739136 (+) Length   559 a.a.
NCBI ID   WP_151173426.1    Uniprot ID   -
Organism   Pseudoalteromonas ruthenica strain LMG 19699     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2732457..2744136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRUTH_RS12705 pilB 2732857..2734548 (+) 1692 WP_151173423.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PRUTH_RS12710 pilC 2734629..2735858 (+) 1230 WP_045979234.1 type II secretion system F family protein Machinery gene
  PRUTH_RS12715 pilD 2735883..2736755 (+) 873 WP_151173424.1 A24 family peptidase Machinery gene
  PRUTH_RS12720 coaE 2736763..2737368 (+) 606 WP_151173425.1 dephospho-CoA kinase -
  PRUTH_RS12725 pilB 2737457..2739136 (+) 1680 WP_151173426.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PRUTH_RS12730 yacG 2739214..2739453 (+) 240 WP_151173427.1 DNA gyrase inhibitor YacG -
  PRUTH_RS12735 - 2739462..2740241 (-) 780 WP_371741513.1 YggN family protein -
  PRUTH_RS12740 - 2740365..2741183 (-) 819 WP_257220938.1 ABC transporter substrate-binding protein -
  PRUTH_RS12745 - 2741253..2743067 (-) 1815 WP_257220939.1 bifunctional diguanylate cyclase/phosphodiesterase -
  PRUTH_RS12750 - 2743137..2743655 (-) 519 WP_151173428.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62164.65 Da        Isoelectric Point: 5.9418

>NTDB_id=246643 PRUTH_RS12725 WP_151173426.1 2737457..2739136(+) (pilB) [Pseudoalteromonas ruthenica strain LMG 19699]
MNINSPLIRKFITLGRIDSDTVKAHQEQSRCAAETIVRSSNMSNQELLAQCVELFRTPYIDLSSFDPRSIDKHLLNEQLI
RRHHMLPISTKGNKLFLAASDPTDYGAFENYEFSTGLQCEVVLADYRQIDNLIEQLFDATHTLKLSQEDSDELSHLAKQD
YDNHHPEQGNEDDDAPIIVYINKILRDAIRRGASDLHFEPYDGDYRIRFRIDGVLHEMANPPYALSTRLAARIKVMSKLD
ISEKRKPQDGRIKLTISPRNSIDFRVSTLPTMWGEKIVMRVLDSSNATLGIDALGYEEEQKQLYLHALQQPQGMILVTGP
TGSGKTVSLYSGLSILNTPERNISTAEDPVEINVKGINQVQINPKAELTFANALRAFLRQDPDVVMVGEIRDLETAEVAI
KAAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSVSLIIAQRLARRLCKYCKEPEQLPTEELIKQGFDANSVAQVK
LYQAKGCDQCTDGYKGRVGIYEVLEVSADIAKVIVSGGSSIDIAETAKEAGCATLRQSGLRKAAEGITSLSEINRVTRL

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=246643 PRUTH_RS12725 WP_151173426.1 2737457..2739136(+) (pilB) [Pseudoalteromonas ruthenica strain LMG 19699]
ATGAATATTAACTCACCTTTGATTCGTAAATTTATTACCTTAGGACGTATTGACAGCGACACAGTAAAGGCGCACCAAGA
GCAAAGCCGCTGCGCAGCAGAGACCATAGTCCGTAGCAGCAATATGAGTAATCAAGAACTGTTGGCACAATGTGTTGAAC
TCTTTCGCACTCCGTATATTGATTTGAGCTCCTTTGATCCCAGAAGCATAGACAAGCACTTGCTAAATGAGCAGTTAATT
CGTCGTCATCATATGCTGCCCATTTCTACCAAGGGCAACAAGCTATTTCTCGCCGCCTCAGACCCTACTGACTACGGTGC
TTTTGAAAATTACGAGTTTAGTACCGGGCTTCAATGTGAAGTGGTGCTGGCTGATTATCGGCAAATAGATAACCTCATCG
AGCAGCTGTTCGATGCCACCCACACACTGAAGCTATCGCAAGAGGACAGCGACGAACTCAGCCACCTCGCCAAACAAGAC
TATGATAATCATCATCCGGAACAGGGCAATGAGGATGACGACGCCCCAATTATTGTCTACATCAATAAGATTCTCCGTGA
TGCCATACGCCGAGGTGCTTCTGATTTACACTTTGAGCCCTATGATGGCGACTACCGCATACGCTTTCGTATTGATGGTG
TGTTACATGAGATGGCCAATCCACCTTATGCGCTGTCCACTCGCCTTGCAGCCCGCATCAAAGTAATGTCTAAACTAGAT
ATTTCGGAAAAGCGCAAACCCCAAGATGGCCGCATTAAGCTTACGATTTCGCCGCGTAATAGCATTGATTTTCGGGTCAG
TACGTTGCCAACCATGTGGGGGGAAAAAATTGTTATGCGGGTGCTTGATTCCTCCAATGCCACTTTAGGAATTGACGCGC
TCGGTTATGAGGAAGAGCAAAAGCAACTTTATCTGCACGCACTGCAACAACCTCAAGGCATGATTCTTGTCACCGGCCCC
ACTGGCTCCGGAAAGACAGTCTCTCTTTACTCAGGCTTAAGCATTTTAAATACACCTGAGCGGAATATCTCTACAGCCGA
AGATCCGGTCGAAATTAACGTTAAGGGCATAAATCAAGTACAAATAAACCCTAAAGCTGAGCTTACGTTCGCTAACGCTC
TTCGCGCCTTCTTACGTCAAGACCCTGATGTAGTAATGGTGGGCGAAATAAGGGACTTAGAAACGGCTGAAGTCGCCATC
AAAGCCGCACAGACCGGACATTTGGTGCTCTCCACCCTGCACACCAACTCCGCACCTGAGACTATCACACGACTTTTAAA
CATGGGCGTACCGGCGTACAACGTGGCTAGCTCGGTGTCGCTGATCATCGCTCAACGCTTAGCACGCCGCCTTTGCAAAT
ACTGTAAAGAGCCAGAGCAGCTCCCAACGGAAGAACTTATTAAGCAAGGGTTTGATGCTAACTCTGTGGCACAAGTAAAA
TTGTATCAGGCAAAAGGTTGCGACCAGTGTACTGACGGGTACAAAGGCCGGGTGGGCATTTATGAGGTATTAGAAGTTTC
AGCGGATATTGCCAAAGTGATTGTTTCCGGTGGTAGCTCGATAGATATCGCCGAAACAGCCAAGGAAGCAGGCTGTGCGA
CATTGCGTCAATCGGGGCTACGTAAAGCTGCAGAGGGCATTACCTCACTATCTGAGATTAATCGTGTTACGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(183-450)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

56.391

95.17

0.537
  pilB Legionella pneumophila strain ERS1305867

54.098

98.211

0.531
  pilB Acinetobacter baylyi ADP1

52.128

100

0.526
  pilB Acinetobacter baumannii D1279779

55.019

92.665

0.51
  pilB Vibrio campbellii strain DS40M4

52.82

95.17

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

53.03

94.454

0.501
  pilF Neisseria gonorrhoeae MS11

49.031

92.308

0.453
  pilF Thermus thermophilus HB27

38.283

100

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.964

100

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

39.692

92.844

0.369

Multiple sequence alignment