Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CCX46_RS25970 Genome accession   NZ_CP022411
Coordinates   5700006..5701223 (+) Length   405 a.a.
NCBI ID   WP_127929816.1    Uniprot ID   -
Organism   Pseudomonas sp. RU47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5695006..5706223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX46_RS25950 (CCX46_25485) - 5696390..5696686 (+) 297 WP_127929813.1 DUF2845 domain-containing protein -
  CCX46_RS25955 (CCX46_25490) - 5696990..5697346 (-) 357 WP_127929814.1 BON domain-containing protein -
  CCX46_RS25960 (CCX46_25495) pilA 5697645..5698076 (-) 432 WP_034153877.1 pilin Machinery gene
  CCX46_RS25965 (CCX46_25500) pilB 5698303..5700003 (+) 1701 WP_127929815.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CCX46_RS25970 (CCX46_25505) pilC 5700006..5701223 (+) 1218 WP_127929816.1 type II secretion system F family protein Machinery gene
  CCX46_RS25975 (CCX46_25510) pilD 5701225..5702097 (+) 873 WP_127929817.1 A24 family peptidase Machinery gene
  CCX46_RS25980 (CCX46_25515) coaE 5702094..5702717 (+) 624 WP_127929818.1 dephospho-CoA kinase -
  CCX46_RS25985 (CCX46_25520) yacG 5702714..5702914 (+) 201 WP_007909902.1 DNA gyrase inhibitor YacG -
  CCX46_RS25990 (CCX46_25525) - 5702921..5703136 (-) 216 WP_007909901.1 hypothetical protein -
  CCX46_RS25995 (CCX46_25530) - 5703202..5703891 (-) 690 WP_127929819.1 energy-coupling factor ABC transporter permease -
  CCX46_RS26000 (CCX46_25535) - 5704084..5704710 (+) 627 WP_127929820.1 DUF1780 domain-containing protein -
  CCX46_RS26005 (CCX46_25540) - 5704707..5705234 (+) 528 WP_127929821.1 MOSC domain-containing protein -
  CCX46_RS26010 (CCX46_25545) - 5705286..5705459 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44067.78 Da        Isoelectric Point: 10.1093

>NTDB_id=239236 CCX46_RS25970 WP_127929816.1 5700006..5701223(+) (pilC) [Pseudomonas sp. RU47]
MAVKAAKISIYAWEGTDRKGSKVTGELSGQNPALIKAQLRKQGISPGKVRKKSASLLSFGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ERLKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSEFMQQWWWAILGALVAAIFGTR
HALKTSQALRDRKDTWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDEMLDKVAGFYEEEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=239236 CCX46_RS25970 WP_127929816.1 5700006..5701223(+) (pilC) [Pseudomonas sp. RU47]
ATGGCGGTCAAGGCAGCGAAAATCAGTATTTATGCCTGGGAAGGTACGGACAGGAAAGGCAGCAAAGTCACCGGTGAACT
CAGTGGACAGAATCCGGCACTGATCAAGGCCCAACTGCGTAAACAGGGGATCAGTCCCGGCAAGGTACGCAAAAAATCCG
CGTCGTTGTTGAGTTTTGGCAAACGCATCAAGGCCCAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGTTGCTGCAGTCGTTCGACATCATTGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAGCTGGTGGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCCACAGCCCTGCGCAAGAAGCCGCAGTATTTCGACGAGTTGTATT
GCAACCTGGTCGATGCCGGCGAGCAGTCCGGCGCCCTCGACACGCTGCTGGAACGGGTCGCAACCTATAAGGAAAAAAGC
GAACGGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCCACCGCCGTGGTGTTGGTGGCGGCAGTCGTGACCGGGAT
CCTGCTGGTCAAAGTGGTGCCGCAATTCCAGTCGGTATTCTCGGGGTTCGGTGCAGAGCTACCGGCCTTCACCCTGATGG
TGATCAGCCTTTCGGAATTCATGCAGCAATGGTGGTGGGCGATTCTCGGCGCGCTGGTCGCGGCGATTTTCGGCACCCGT
CACGCACTGAAAACCTCCCAGGCGCTACGTGATCGCAAAGACACCTGGCTGTTGAAACTGCCCTTGGTCGGCACGCTGAT
GTACAAGTCAGCGGTAGCCCGCTTCGCTCGAACCCTGTCGACCACGTTTGCTGCCGGTGTGCCATTGGTGGAAGCGCTCG
ACTCGGTGGCCGGCGCTACCGGCAATGTGGTGTTCAAGCGTGCCGTGCTGCGCATTCGCCAAGACGTCTCCACCGGCATG
CAGCTGAATTTTTCGATGCGCAGCACCGGAGTCTTTCCCAACATGGCCGTGCAGATGACCGCGATTGGCGAAGAGTCGGG
GGCGCTAGATGAAATGCTCGACAAGGTTGCAGGCTTTTACGAAGAAGAAGTGGATAACATGGTCGATAACCTCACCAGCC
TCATGGAGCCGTTCATCATGGTCGTGCTGGGGGTGATCGTCGGCGGGCTGGTGGTGGCCATGTACCTGCCGATTTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baumannii D1279779

59.559

100

0.6
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilC Vibrio cholerae strain A1552

44.444

97.778

0.435
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Thermus thermophilus HB27

38.903

99.012

0.385

Multiple sequence alignment