Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CCX46_RS25960 Genome accession   NZ_CP022411
Coordinates   5697645..5698076 (-) Length   143 a.a.
NCBI ID   WP_034153877.1    Uniprot ID   A0A7Z2REX4
Organism   Pseudomonas sp. RU47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5692645..5703076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX46_RS25945 (CCX46_25480) - 5693439..5696390 (+) 2952 WP_127929812.1 DUF748 domain-containing protein -
  CCX46_RS25950 (CCX46_25485) - 5696390..5696686 (+) 297 WP_127929813.1 DUF2845 domain-containing protein -
  CCX46_RS25955 (CCX46_25490) - 5696990..5697346 (-) 357 WP_127929814.1 BON domain-containing protein -
  CCX46_RS25960 (CCX46_25495) pilA 5697645..5698076 (-) 432 WP_034153877.1 pilin Machinery gene
  CCX46_RS25965 (CCX46_25500) pilB 5698303..5700003 (+) 1701 WP_127929815.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CCX46_RS25970 (CCX46_25505) pilC 5700006..5701223 (+) 1218 WP_127929816.1 type II secretion system F family protein Machinery gene
  CCX46_RS25975 (CCX46_25510) pilD 5701225..5702097 (+) 873 WP_127929817.1 A24 family peptidase Machinery gene
  CCX46_RS25980 (CCX46_25515) coaE 5702094..5702717 (+) 624 WP_127929818.1 dephospho-CoA kinase -
  CCX46_RS25985 (CCX46_25520) yacG 5702714..5702914 (+) 201 WP_007909902.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14323.47 Da        Isoelectric Point: 8.4936

>NTDB_id=239234 CCX46_RS25960 WP_034153877.1 5697645..5698076(-) (pilA) [Pseudomonas sp. RU47]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISALKVPFEDTINQGTDPTLTLVAGSATAATSNCT
LAASGTASTGAGTITCTLLNAPGPVLSKTITLTRSSGTGAGVWTCGTTVAPDYSPKGCTASGT

Nucleotide


Download         Length: 432 bp        

>NTDB_id=239234 CCX46_RS25960 WP_034153877.1 5697645..5698076(-) (pilA) [Pseudomonas sp. RU47]
ATGAAAAAACAACAAGGTTTTACTCTGATCGAGCTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACCATCGCCTT
GCCGCAGTATTCCAAGTATCAGGCGCGGTCGAAGGTAACGGCGGGGCTGGCGGAGATCAGTGCGCTGAAGGTGCCGTTTG
AAGACACCATCAATCAAGGCACCGATCCTACGCTGACATTGGTTGCGGGTAGTGCGACGGCGGCGACATCCAACTGCACA
CTCGCCGCATCCGGTACTGCATCCACCGGTGCCGGAACCATCACTTGCACACTGCTCAATGCCCCGGGTCCGGTGCTGAG
CAAGACCATCACCCTGACTCGTTCTTCAGGGACCGGCGCGGGTGTATGGACCTGCGGTACCACCGTTGCTCCGGATTATT
CCCCTAAAGGCTGCACCGCCAGCGGTACATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2REX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae C6706

45.395

100

0.483
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.395

100

0.483
  pilA Vibrio cholerae strain A1552

45.395

100

0.483
  pilA Acinetobacter baumannii strain A118

47.887

99.301

0.476
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.928

96.503

0.434
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.434
  comP Acinetobacter baylyi ADP1

39.61

100

0.427
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.478

96.503

0.42
  pilA Vibrio parahaemolyticus RIMD 2210633

40.278

100

0.406
  pilA2 Legionella pneumophila str. Paris

40.714

97.902

0.399
  pilA2 Legionella pneumophila strain ERS1305867

40.714

97.902

0.399
  pilA Ralstonia pseudosolanacearum GMI1000

34.783

100

0.392

Multiple sequence alignment