Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CCX46_RS25965 Genome accession   NZ_CP022411
Coordinates   5698303..5700003 (+) Length   566 a.a.
NCBI ID   WP_127929815.1    Uniprot ID   -
Organism   Pseudomonas sp. RU47     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5693303..5705003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX46_RS25945 (CCX46_25480) - 5693439..5696390 (+) 2952 WP_127929812.1 DUF748 domain-containing protein -
  CCX46_RS25950 (CCX46_25485) - 5696390..5696686 (+) 297 WP_127929813.1 DUF2845 domain-containing protein -
  CCX46_RS25955 (CCX46_25490) - 5696990..5697346 (-) 357 WP_127929814.1 BON domain-containing protein -
  CCX46_RS25960 (CCX46_25495) pilA 5697645..5698076 (-) 432 WP_034153877.1 pilin Machinery gene
  CCX46_RS25965 (CCX46_25500) pilB 5698303..5700003 (+) 1701 WP_127929815.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CCX46_RS25970 (CCX46_25505) pilC 5700006..5701223 (+) 1218 WP_127929816.1 type II secretion system F family protein Machinery gene
  CCX46_RS25975 (CCX46_25510) pilD 5701225..5702097 (+) 873 WP_127929817.1 A24 family peptidase Machinery gene
  CCX46_RS25980 (CCX46_25515) coaE 5702094..5702717 (+) 624 WP_127929818.1 dephospho-CoA kinase -
  CCX46_RS25985 (CCX46_25520) yacG 5702714..5702914 (+) 201 WP_007909902.1 DNA gyrase inhibitor YacG -
  CCX46_RS25990 (CCX46_25525) - 5702921..5703136 (-) 216 WP_007909901.1 hypothetical protein -
  CCX46_RS25995 (CCX46_25530) - 5703202..5703891 (-) 690 WP_127929819.1 energy-coupling factor ABC transporter permease -
  CCX46_RS26000 (CCX46_25535) - 5704084..5704710 (+) 627 WP_127929820.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62523.49 Da        Isoelectric Point: 5.9454

>NTDB_id=239235 CCX46_RS25965 WP_127929815.1 5698303..5700003(+) (pilB) [Pseudomonas sp. RU47]
MNDIALSGLAKQLVQAELLTEKSAQQASQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDTNASGLEDMADVDLDG
LDVESIDDNKQDAIAGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSRSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPVQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKAIEIPRETLIKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDIQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=239235 CCX46_RS25965 WP_127929815.1 5698303..5700003(+) (pilB) [Pseudomonas sp. RU47]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCAAGC
CTCGCAACAGGCCCAGCGTAATCGCTTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCAAGAGCTCACAGGTCG
CCGAAATCGCCTCCGAGCATTTCGGCATGGCCTTGCTTGATCTCAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCCTGTGGCGACGTGGCAACAAGCTGTTCGTAGGCATTTCCGA
CCCGAGCAATCATCAAGCGATCAACGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCAGCGATGCCATCGAAAAATTCTTCGACACCAACGCCAGCGGTCTGGAGGACATGGCCGATGTCGATCTCGATGGC
CTCGACGTCGAATCCATTGACGACAACAAACAGGATGCCATCGCCGGTCTCGACGCCGACGATGCGCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTCGACGCGATCAAGAGCGGCTCTTCCGACTTGCACTTCGAACCCTACGAGAAGAACTACCGGG
TACGGGTGCGCACCGACGGCATTTTGCGCGAGGTGGCCAAGCCACCGATTCAACTGGCCGGGCGCATCGCCGCGCGCCTG
AAAGTCATGGCCAGCCTCGATATCTCGGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAG
GTCGATCGATTTTCGGGTCAACACGCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CACAGATCGGCATCGACGCGCTCGGCTATGAGCCCGTGCAGAAAGACCTGTATATGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGGCCCACCGGTTCGGGTAAAACCGTTTCGCTGTACACCGGCCTGAATATTCTCAACACCGTCGA
CATCAATATCTCCACCGCCGAGGACCCGGTGGAGATCAATATGGAAGGCATCAACCAGGTCAACGTCAACCCCAAACAGG
GCCTCGATTTTGCCCAGGCGCTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTTGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAAGCCGCGCAGACCGGTCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACGCGCCTGCACAACATGGGCATTCCCGGCTTCAACATTGCCACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCACGCAAGCTCTGCAGCCATTGCAAGAAAGCCATCGAGATCCCCCGCGAAACCTTGATCAAAGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGAGCTGCAACGCTTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACATTCAAA
TGCGCCGCGACGGCTTCGACGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.156

99.647

0.58
  pilB Acinetobacter baylyi ADP1

58.082

99.47

0.578
  pilB Legionella pneumophila strain ERS1305867

56.46

99.823

0.564
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

49.822

99.293

0.495
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.736

93.816

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.519

0.362
  pilF Thermus thermophilus HB27

36.738

98.587

0.362

Multiple sequence alignment