Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   B6N58_RS10340 Genome accession   NZ_CP020614
Coordinates   2327597..2328007 (-) Length   136 a.a.
NCBI ID   WP_045098775.1    Uniprot ID   A0A098GEE0
Organism   Legionella micdadei strain NZ2015     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2322597..2333007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6N58_RS10305 (B6N58_10305) - 2322875..2323063 (-) 189 WP_045098782.1 hypothetical protein -
  B6N58_RS10310 (B6N58_10310) - 2323303..2323860 (+) 558 WP_045098781.1 hypothetical protein -
  B6N58_RS10315 (B6N58_10315) - 2323920..2324627 (-) 708 WP_045098780.1 hypothetical protein -
  B6N58_RS10320 (B6N58_10320) - 2324795..2325169 (+) 375 WP_045098779.1 hypothetical protein -
  B6N58_RS14810 - 2325269..2325421 (-) 153 WP_164493052.1 hypothetical protein -
  B6N58_RS10325 (B6N58_10325) - 2325556..2325831 (-) 276 WP_045098778.1 acylphosphatase -
  B6N58_RS10330 (B6N58_10330) - 2325868..2327025 (-) 1158 WP_045098777.1 hypothetical protein -
  B6N58_RS10335 (B6N58_10335) - 2327189..2327491 (+) 303 WP_045098776.1 hypothetical protein -
  B6N58_RS10340 (B6N58_10340) pilA2 2327597..2328007 (-) 411 WP_045098775.1 pilin Machinery gene
  B6N58_RS10345 (B6N58_10345) - 2328092..2329951 (-) 1860 WP_045098774.1 hypothetical protein -
  B6N58_RS10350 (B6N58_10350) - 2330187..2330501 (+) 315 WP_045098773.1 BolA family protein -
  B6N58_RS10355 (B6N58_10355) - 2330494..2330928 (+) 435 WP_216637042.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  B6N58_RS10360 (B6N58_10360) - 2331189..2332598 (+) 1410 WP_045100566.1 amino acid permease -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14535.88 Da        Isoelectric Point: 7.8466

>NTDB_id=224970 B6N58_RS10340 WP_045098775.1 2327597..2328007(-) (pilA2) [Legionella micdadei strain NZ2015]
MKQKGLTLIELMIVIAILGILISIAIPAYQDYTVRARVAEGLNMAQTAKLAVAEAAMTNHALPDSQEATHYTSPAPTANV
QSIVIGPKGEITITYTELAGNGTLVLVPTLQANGDLTWACNKGTLKRQYRPATCRD

Nucleotide


Download         Length: 411 bp        

>NTDB_id=224970 B6N58_RS10340 WP_045098775.1 2327597..2328007(-) (pilA2) [Legionella micdadei strain NZ2015]
ATGAAACAAAAGGGATTAACACTTATTGAGTTAATGATTGTCATTGCTATCTTAGGCATTCTCATTTCGATTGCGATCCC
TGCTTATCAAGATTACACCGTTAGAGCTCGTGTAGCTGAAGGTTTAAATATGGCCCAGACGGCAAAATTAGCTGTCGCTG
AAGCGGCAATGACTAATCATGCTTTACCTGACTCCCAGGAGGCAACCCACTACACCAGCCCCGCGCCGACTGCAAATGTT
CAATCGATTGTAATTGGTCCCAAAGGAGAAATTACTATTACTTATACTGAGCTTGCAGGTAACGGGACTTTAGTGTTAGT
TCCTACTCTACAGGCAAATGGGGATTTAACGTGGGCATGTAATAAAGGAACACTAAAAAGACAATATCGGCCTGCAACAT
GTAGAGATTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(34-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098GEE0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

68.889

99.265

0.684
  pilA2 Legionella pneumophila str. Paris

68.889

99.265

0.684
  pilA Ralstonia pseudosolanacearum GMI1000

45.342

100

0.537
  comP Acinetobacter baylyi ADP1

44.138

100

0.471
  pilE Neisseria gonorrhoeae strain FA1090

34.783

100

0.412
  pilE Neisseria gonorrhoeae MS11

34.177

100

0.397
  pilA/pilA1 Eikenella corrodens VA1

35.526

100

0.397
  pilA Pseudomonas aeruginosa PAK

34.228

100

0.375
  pilA Vibrio cholerae strain A1552

33.793

100

0.36
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

33.793

100

0.36
  pilA Vibrio cholerae C6706

33.793

100

0.36

Multiple sequence alignment