Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB   Type   Machinery gene
Locus tag   GTHT12_RS16425 Genome accession   NZ_CP020030
Coordinates   3256329..3256676 (-) Length   115 a.a.
NCBI ID   WP_008880386.1    Uniprot ID   A4IT00
Organism   Geobacillus thermodenitrificans strain T12     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context


Location: 3251329..3261676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTHT12_RS16410 (GTHT12_02669) - 3253131..3254765 (-) 1635 WP_087960262.1 peptidoglycan-binding protein -
  GTHT12_RS16415 (GTHT12_02670) - 3255075..3255575 (-) 501 WP_008880384.1 hypothetical protein -
  GTHT12_RS16420 (GTHT12_02671) - 3255789..3256199 (+) 411 WP_029761384.1 helix-turn-helix transcriptional regulator -
  GTHT12_RS16425 (GTHT12_02672) ssbB 3256329..3256676 (-) 348 WP_008880386.1 single-stranded DNA-binding protein Machinery gene
  GTHT12_RS16430 (GTHT12_02673) - 3256960..3257400 (+) 441 WP_008880387.1 YwpF-like family protein -
  GTHT12_RS16435 (GTHT12_02674) - 3257587..3258258 (-) 672 WP_008880388.1 class D sortase -
  GTHT12_RS16440 (GTHT12_02675) - 3258265..3259233 (-) 969 WP_081157468.1 processed acidic surface protein -
  GTHT12_RS16445 (GTHT12_02676) - 3259343..3260719 (-) 1377 WP_011888254.1 aspartate kinase -
  GTHT12_RS16450 (GTHT12_02677) - 3260870..3261304 (-) 435 WP_008880391.1 DUF1284 domain-containing protein -

Sequence


Protein


Download         Length: 115 a.a.        Molecular weight: 12957.77 Da        Isoelectric Point: 8.5040

>NTDB_id=220547 GTHT12_RS16425 WP_008880386.1 3256329..3256676(-) (ssbB) [Geobacillus thermodenitrificans strain T12]
MINQVVLVGRLTKDPELRYTAEGTAVTTVILAVARNFRNAEGGIDADFVPCVLWRKTAEHTAHYCQKGSMVAVTGRIQTR
RYENKDGQRVYVTEVVADSVQFLHSGKVREWPEHV

Nucleotide


Download         Length: 348 bp        

>NTDB_id=220547 GTHT12_RS16425 WP_008880386.1 3256329..3256676(-) (ssbB) [Geobacillus thermodenitrificans strain T12]
ATGATCAATCAAGTCGTACTTGTCGGCCGATTGACGAAGGATCCAGAGCTTCGTTACACGGCCGAAGGGACGGCCGTTAC
AACCGTGATCTTGGCGGTAGCAAGAAATTTTCGCAACGCGGAAGGGGGGATTGATGCTGACTTCGTTCCGTGTGTCCTAT
GGCGGAAAACGGCAGAGCATACTGCCCATTATTGCCAAAAAGGATCGATGGTGGCGGTAACGGGTAGAATCCAGACGCGC
CGTTATGAAAACAAAGACGGCCAGCGCGTCTATGTGACGGAAGTCGTAGCCGATTCAGTTCAGTTTCTCCACTCCGGCAA
AGTGCGAGAATGGCCGGAGCACGTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A4IT00

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Bacillus subtilis subsp. subtilis str. 168

67.273

95.652

0.643

  ssbA Bacillus subtilis subsp. subtilis str. 168

62.5

90.435

0.565

  ssb Latilactobacillus sakei subsp. sakei 23K

60.952

91.304

0.557

  ssbB Streptococcus sobrinus strain NIDR 6715-7

47.368

99.13

0.47

  ssbA Streptococcus mutans UA159

44.737

99.13

0.443

  ssbB/cilA Streptococcus mitis NCTC 12261

43.86

99.13

0.435

  ssbB/cilA Streptococcus pneumoniae TIGR4

43.86

99.13

0.435

  ssbB/cilA Streptococcus mitis SK321

42.982

99.13

0.426

  ssbB/cilA Streptococcus pneumoniae Rx1

42.982

99.13

0.426

  ssbB/cilA Streptococcus pneumoniae D39

42.982

99.13

0.426

  ssbB/cilA Streptococcus pneumoniae R6

42.982

99.13

0.426

  ssbB Lactococcus lactis subsp. cremoris KW2

41.346

90.435

0.374


Multiple sequence alignment