Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PspR32_RS01360 Genome accession   NZ_CP019396
Coordinates   305161..306402 (+) Length   413 a.a.
NCBI ID   WP_232531789.1    Uniprot ID   -
Organism   Pseudomonas sp. R32     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 300161..311402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspR32_RS01345 (PspR32_01345) - 300579..303035 (-) 2457 WP_010222226.1 penicillin-binding protein 1A -
  PspR32_RS01350 (PspR32_01350) pilM 303041..304288 (+) 1248 WP_159387996.1 pilus assembly protein PilM -
  PspR32_RS01355 (PspR32_01355) - 304285..305136 (+) 852 WP_159387997.1 pilus assembly protein PilP -
  PspR32_RS01360 (PspR32_01360) pilQ 305161..306402 (+) 1242 WP_232531789.1 type IV pilus secretin PilQ family protein Machinery gene
  PspR32_RS01365 (PspR32_01365) aroK 306407..306925 (+) 519 WP_010222231.1 shikimate kinase AroK -
  PspR32_RS01370 (PspR32_01370) aroB 307024..308121 (+) 1098 WP_010222232.1 3-dehydroquinate synthase -
  PspR32_RS01375 (PspR32_01375) - 308138..309760 (+) 1623 WP_159387998.1 AAA family ATPase -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 44399.88 Da        Isoelectric Point: 5.5291

>NTDB_id=215129 PspR32_RS01360 WP_232531789.1 305161..306402(+) (pilQ) [Pseudomonas sp. R32]
MGKGGVIAALIGGWLFAGVVAGKQLYGGEPLSLNFQDVEVRTVLQVLADFSGVNLVASDAVQGKVTLRLQDVPWDQALDL
VLRSKGLEQRKDGNVLLVAPRAELAGSAPSRRELIPIHHAKAADIAELFHALMAHEAGGASHGSLSVDERTNTLVASQPP
ERLEELRRLIGELDVPVRQVMIEARIVEANVDFEKALGVRWGGAVQVGKGLSLGSADTFVDLGVDRAASSIGVGLVRNNV
LLDLELSAMEKTGNGEIISQPKVVTADKETARILKGTEVPYQETSGSGATSVTFREASLSLEVTPQITPDNRVIMAVKVT
KDEPDYLNALDHVPAIRKNEVNAKVRINDGETIVIGGVYSTVQSKVVDKVPFFGDVPYVGRLFRRDVLQEKKSELLVFLT
PRIMSDQAIAVSR

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=215129 PspR32_RS01360 WP_232531789.1 305161..306402(+) (pilQ) [Pseudomonas sp. R32]
ATGGGCAAGGGAGGTGTTATTGCAGCGCTGATAGGGGGCTGGCTGTTTGCCGGCGTCGTTGCGGGGAAGCAGCTGTATGG
TGGCGAGCCGCTGTCGCTTAATTTCCAGGATGTCGAGGTACGTACGGTATTGCAGGTGCTGGCCGACTTTTCCGGGGTCA
ACCTGGTGGCCAGTGACGCGGTTCAAGGCAAGGTGACCTTGCGTTTGCAGGATGTGCCCTGGGACCAGGCGTTGGATCTG
GTACTGCGCAGCAAGGGCCTGGAGCAGCGCAAGGACGGCAATGTGCTGCTGGTCGCACCGCGTGCCGAGCTGGCCGGTTC
AGCGCCGTCGCGGCGCGAGCTGATCCCGATCCATCATGCCAAGGCCGCCGATATCGCCGAGTTGTTCCACGCCCTGATGG
CCCATGAGGCCGGGGGCGCCAGCCACGGTTCGCTGAGTGTCGACGAGCGCACCAATACCCTGGTCGCCAGCCAGCCGCCA
GAGCGTCTGGAAGAGCTGCGCAGGCTGATCGGCGAACTGGATGTACCGGTTCGTCAGGTGATGATCGAGGCGCGGATTGT
CGAGGCCAATGTCGATTTCGAAAAGGCCCTGGGGGTGCGTTGGGGTGGCGCCGTGCAGGTGGGCAAGGGTTTGAGCCTCG
GCAGCGCCGATACCTTTGTCGATCTTGGCGTGGATCGGGCCGCGTCCAGCATCGGCGTGGGCCTGGTGCGCAACAACGTA
CTGCTCGACCTGGAGCTCAGCGCCATGGAAAAGACCGGCAATGGCGAGATCATCTCCCAGCCCAAGGTGGTTACCGCCGA
CAAGGAAACCGCGCGGATCCTCAAGGGCACCGAGGTGCCGTATCAGGAAACCAGCGGCAGCGGCGCCACGTCCGTGACCT
TCCGTGAGGCATCGCTGTCTCTGGAGGTGACCCCGCAGATCACCCCGGACAACCGGGTGATCATGGCGGTCAAGGTGACC
AAGGACGAGCCCGACTACCTCAATGCCCTTGATCACGTGCCGGCGATCCGCAAGAACGAGGTCAACGCCAAGGTGCGCAT
CAATGATGGCGAGACCATCGTGATCGGCGGCGTGTACTCGACGGTGCAAAGTAAAGTTGTCGACAAGGTGCCATTTTTCG
GCGATGTGCCGTATGTTGGGCGGCTGTTTCGTCGCGATGTTCTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACT
CCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(114-179)

Secretin and TonB N terminus short domain (STN)

(54-100)

Bacterial type II and III secretion system protein (Secretin)

(248-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

60.321

100

0.637
  comQ Acinetobacter baylyi ADP1

42.539

100

0.462
  pilQ Acinetobacter baumannii strain A118

40.741

100

0.453
  pilQ Acinetobacter baumannii D1279779

40.741

100

0.453
  pilQ Legionella pneumophila strain Lp02

43.23

100

0.441
  pilQ Legionella pneumophila strain ERS1305867

43.23

100

0.441
  pilQ Vibrio campbellii strain DS40M4

42.118

100

0.433
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.866

100

0.424
  pilQ Vibrio cholerae strain A1552

41.866

100

0.424
  pilQ Neisseria meningitidis M1080

36.534

100

0.378
  pilQ Neisseria meningitidis MC58

36.15

100

0.373
  pilQ Neisseria gonorrhoeae strain FA1090

36.15

100

0.373

Multiple sequence alignment