Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BVG12_RS24670 Genome accession   NZ_CP019038
Coordinates   5058381..5059571 (+) Length   396 a.a.
NCBI ID   WP_075794690.1    Uniprot ID   -
Organism   Massilia putida strain 6NM-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5053381..5064571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG12_RS35890 - 5054369..5054548 (+) 180 Protein_4418 EAL domain-containing protein -
  BVG12_RS34600 - 5054609..5054902 (-) 294 WP_075794684.1 hypothetical protein -
  BVG12_RS24645 - 5054895..5055299 (-) 405 WP_075794685.1 phage holin family protein -
  BVG12_RS24650 - 5055299..5055619 (-) 321 WP_075794686.1 YqjD family protein -
  BVG12_RS24655 ubiA 5055781..5056635 (-) 855 WP_075794687.1 4-hydroxybenzoate octaprenyltransferase -
  BVG12_RS24660 - 5056802..5057314 (+) 513 WP_075794688.1 hypothetical protein -
  BVG12_RS24665 - 5057292..5058236 (-) 945 WP_075794689.1 hydrogen peroxide-inducible genes activator -
  BVG12_RS24670 pilU 5058381..5059571 (+) 1191 WP_075794690.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BVG12_RS24675 rfaE2 5059581..5060060 (+) 480 WP_075794691.1 D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase -
  BVG12_RS24680 trmL 5060070..5060540 (-) 471 WP_075794692.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  BVG12_RS35295 - 5060613..5061485 (-) 873 WP_229503708.1 ComF family protein -
  BVG12_RS24690 - 5061544..5062455 (+) 912 WP_229503709.1 methyltransferase domain-containing protein -
  BVG12_RS24695 coxB 5063081..5064208 (+) 1128 WP_370662850.1 cytochrome c oxidase subunit II -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 43662.02 Da        Isoelectric Point: 6.1884

>NTDB_id=212710 BVG12_RS24670 WP_075794690.1 5058381..5059571(+) (pilU) [Massilia putida strain 6NM-7]
MSSTFGPAEAQAYMHKLLTVMNHQGGSDLFISADFPPSMKHQGAMKPLSNQRLTGEVTRALALSLMNERQRAEFEAEMEC
NFAISLPGVCRFRVNVFVQQQSVGMVVRTIASEIPNFEKLDLPEVLKDVVMTKRGLVLVVGGTGSGKSTTLAAMIDYRNS
NSAGHIITVEDPVEYVHKNKSCLITHREVGVDTLSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLVRTEDGKGRKAAIEILLNTPTIGEMILKGSFQSIKEIMQKS
RELGMCTFDQALFELYNKGFISYDEAIRNADSANGLRLQIKLSGERKAPGEGGGAAKPAELSMLMDEPEEEPPAAS

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=212710 BVG12_RS24670 WP_075794690.1 5058381..5059571(+) (pilU) [Massilia putida strain 6NM-7]
ATGTCTTCGACATTCGGTCCGGCGGAAGCGCAGGCCTATATGCACAAGCTGCTCACGGTGATGAATCACCAGGGCGGCTC
CGACCTGTTCATCTCGGCCGATTTCCCGCCCAGCATGAAGCACCAGGGCGCCATGAAACCCCTGAGCAACCAGCGCCTGA
CGGGCGAGGTCACGCGCGCGCTCGCCTTGTCGCTGATGAACGAGCGGCAGCGCGCCGAGTTCGAGGCCGAGATGGAGTGC
AATTTCGCCATCTCCCTGCCGGGCGTGTGCCGTTTCCGCGTGAACGTATTCGTGCAGCAGCAGAGCGTCGGCATGGTCGT
GCGTACGATCGCGTCGGAAATCCCGAACTTCGAAAAGCTCGACCTGCCCGAGGTCTTGAAAGATGTCGTGATGACGAAGC
GGGGCCTCGTGCTCGTGGTCGGCGGCACGGGGTCCGGCAAGTCGACGACGCTCGCGGCGATGATCGATTACCGCAACAGC
AATTCGGCCGGCCACATCATCACGGTCGAGGACCCGGTCGAATACGTCCACAAGAACAAAAGCTGCCTCATCACGCACCG
CGAGGTCGGCGTCGACACGCTGTCGTGGCATAACGCGCTGAAGAACACACTGCGCCAGGCGCCGGATGTGATCCTGATCG
GTGAGATCCGCGACACGGAAACGATGGAGCACGCGATCGCGTTCGCCGAAACGGGCCACCTGTGCCTGGGCACACTGCAC
GCGAACAATGCCAACCAGACAATGGACCGCATCATCAACTTCTTCCCGGAAGAGCGTAGAAACCAGCTCTTGATGGACCT
GTCGTCGAACCTGCGCGCCATCGTGTCGCAGCGCCTGGTCCGCACGGAAGACGGCAAGGGCCGCAAGGCCGCCATCGAAA
TCCTGCTGAACACGCCGACCATCGGCGAGATGATCCTGAAAGGCAGCTTCCAGAGCATCAAGGAGATCATGCAGAAGTCG
CGCGAGCTGGGCATGTGCACGTTCGACCAGGCGCTGTTCGAGCTCTACAACAAAGGCTTCATCAGCTACGACGAAGCCAT
CCGCAACGCCGATTCCGCGAACGGCCTGCGCCTGCAGATCAAGCTGTCGGGCGAAAGAAAGGCGCCGGGGGAGGGCGGCG
GAGCCGCCAAGCCGGCCGAGCTGTCGATGCTGATGGACGAGCCGGAAGAGGAACCGCCGGCCGCGAGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60

88.384

0.53
  pilU Acinetobacter baylyi ADP1

52.291

93.687

0.49
  pilU Vibrio cholerae strain A1552

48

94.697

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.132

91.919

0.396
  pilT Pseudomonas aeruginosa PAK

44.411

83.586

0.371
  pilT Legionella pneumophila strain Lp02

44.954

82.576

0.371
  pilT Legionella pneumophila strain ERS1305867

44.954

82.576

0.371
  pilT Pseudomonas stutzeri DSM 10701

43.582

84.596

0.369
  pilT Acinetobacter baylyi ADP1

43.505

83.586

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.333

83.333

0.361
  pilT Vibrio cholerae strain A1552

43.333

83.333

0.361

Multiple sequence alignment