Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K08M4_RS01275 Genome accession   NZ_CP017916
Coordinates   273385..275103 (+) Length   572 a.a.
NCBI ID   WP_086048621.1    Uniprot ID   A0AA34XM98
Organism   Vibrio syngnathi strain K08M4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268385..280103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K08M4_RS01255 (K08M4_02430) pilM 270599..271624 (+) 1026 WP_086048617.1 type IV pilus assembly protein PilM Machinery gene
  K08M4_RS01260 (K08M4_02440) pilN 271608..272222 (+) 615 WP_086048618.1 PilN domain-containing protein Machinery gene
  K08M4_RS01265 (K08M4_02450) pilO 272215..272844 (+) 630 WP_086048619.1 type 4a pilus biogenesis protein PilO Machinery gene
  K08M4_RS01270 (K08M4_02460) pilP 272831..273349 (+) 519 WP_086048620.1 pilus assembly protein PilP Machinery gene
  K08M4_RS01275 (K08M4_02470) pilQ 273385..275103 (+) 1719 WP_086048621.1 type IV pilus secretin PilQ family protein Machinery gene
  K08M4_RS01280 (K08M4_02480) aroK 275280..275798 (+) 519 WP_009848907.1 shikimate kinase AroK -
  K08M4_RS01285 (K08M4_02490) aroB 275823..276911 (+) 1089 WP_086048622.1 3-dehydroquinate synthase -
  K08M4_RS01290 (K08M4_02500) - 276991..278508 (+) 1518 WP_086048623.1 AAA family ATPase -
  K08M4_RS01295 (K08M4_02510) - 278578..279438 (+) 861 WP_086048624.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62265.10 Da        Isoelectric Point: 4.4058

>NTDB_id=204042 K08M4_RS01275 WP_086048621.1 273385..275103(+) (pilQ) [Vibrio syngnathi strain K08M4]
MIKGLSKLINKGLQSLVVSVMLVVSVFSYAESLPNQLENIDFRVNKEKDAVIIIELATSTAVVDVQRAQEGLSIELLNTQ
VNDDKLYLLDVKDFATSVEGIEVYKETPSTRLLATIAGDYQYEYSLKGRFIEVVISRPTIDEVTIEKNVLEKEGKVISIN
FQDIPVRNVLQLIADYNDFNLVVSDSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEK
SRLEEELGELKSEIIKINFAKATDIADMIGGEGAVSMLSERGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQ
IEARIVTVTEGNLDELGVRWGVSSTNGSFKVGGSIEGNHPLQITPYDDNGGNSAIDDFLNVNLGATSPSASSIAFQVAKL
GSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLD
LSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSQVDKVPLLGDLPVLGALFRRSYENVGKSE
LLIFVTPKVVIQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=204042 K08M4_RS01275 WP_086048621.1 273385..275103(+) (pilQ) [Vibrio syngnathi strain K08M4]
ATGATTAAAGGACTAAGTAAGTTAATAAACAAAGGGCTGCAAAGCCTAGTTGTGTCTGTGATGTTGGTGGTTAGCGTGTT
CAGCTATGCCGAGAGCTTGCCAAATCAATTGGAAAATATTGATTTTAGGGTCAACAAGGAGAAAGACGCCGTCATCATTA
TTGAATTGGCAACCAGCACTGCTGTGGTGGATGTTCAACGTGCTCAGGAAGGTTTAAGTATCGAGTTACTCAATACTCAG
GTGAATGACGATAAGCTTTACCTCCTGGATGTGAAGGACTTCGCGACCTCAGTTGAAGGCATTGAGGTGTATAAGGAAAC
GCCCAGTACGCGATTGTTGGCAACGATCGCTGGTGACTATCAATATGAATACAGCTTAAAAGGTCGCTTTATTGAGGTAG
TGATCAGTAGGCCTACTATTGATGAAGTGACAATAGAGAAAAATGTTTTAGAAAAAGAAGGCAAGGTGATATCGATTAAC
TTCCAAGATATCCCTGTACGTAATGTTTTGCAGCTTATTGCTGATTACAACGATTTCAATCTCGTTGTTTCTGATTCAGT
CACGGGTAATCTGACTTTACGTTTAGATGGTGTGCCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAGGGGTTGG
ATAAGCGTGTTGATGGCAACGTTATCTTGGTGGCACCTAAAGCTGAGCTCGACCTTAGAGAGCAACAAGCGCTAGAGAAA
TCTCGTTTGGAAGAAGAGCTAGGTGAACTCAAGTCGGAAATCATCAAGATTAACTTTGCCAAAGCCACCGACATTGCCGA
CATGATTGGTGGGGAAGGGGCGGTAAGCATGTTGTCCGAGCGAGGCTCGATTACCATTGATGAGCGAACTAACTCTCTGT
TGATCCGTGAATTAGAAGAAAACATTGCGGTGATACGAGGCATTATTGAATCGCTAGACATTCCCGTGAAGCAAGTTCAG
ATAGAAGCACGTATTGTCACTGTTACTGAGGGTAATCTTGATGAACTAGGAGTGCGTTGGGGCGTTTCCTCGACCAACGG
AAGCTTCAAAGTTGGCGGCTCGATAGAGGGCAACCATCCATTGCAAATTACGCCGTATGACGATAATGGTGGGAATAGTG
CGATTGACGATTTCCTCAACGTTAATTTAGGAGCAACTTCCCCGAGCGCGTCGAGTATTGCGTTTCAGGTAGCCAAGCTG
GGTTCTGATACCTTGCTCGATCTTGAATTGTCAGCACTGCAACAAGAGTCGAAAGCAGAGATTATTTCTAGCCCACGCTT
AATTACCACCAATAAAAAACCCGCTTATATTGAGCAAGGTACTGAGATCCCTTATTTAGAGTCTTCTTCAAGTGGTGCTA
CTTCAGTCGCATTTAAGAAAGCGGTATTGAGTCTCAAGGTGACTCCGCAGATCACCCCCGACAATCGTTTGGTATTGGAT
TTGAGTGTGACTCAAGATAGGCCGGGGCAAGTGGTGAAAACCGGTACCGGTGAAGCTGTCGCTATTGACACGCAAAGAAT
TGGGACGCAAGTGCTTGTTAATAATGGTGAAACGGTCGTGTTAGGTGGTATTTTTCAACACAGTGTGAGCAGCCAAGTTG
ATAAAGTTCCACTGTTAGGAGACCTTCCTGTTCTTGGCGCATTGTTCCGTCGTAGCTATGAAAATGTAGGTAAAAGTGAA
CTACTTATTTTTGTCACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)

Bacterial type II and III secretion system protein (Secretin)

(412-570)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

78.049

100

0.783
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

78.049

100

0.783
  pilQ Vibrio campbellii strain DS40M4

78.623

96.503

0.759
  pilQ Legionella pneumophila strain ERS1305867

42.674

95.455

0.407
  pilQ Legionella pneumophila strain Lp02

42.674

95.455

0.407
  pilQ Pseudomonas aeruginosa PAK

40.934

97.378

0.399
  comQ Acinetobacter baylyi ADP1

39.576

98.951

0.392
  pilQ Acinetobacter baumannii D1279779

37.889

100

0.383
  pilQ Acinetobacter baumannii strain A118

37.889

100

0.383

Multiple sequence alignment